Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC

Yang, Jianwen; Wang, Zongnan; Zhou, Tong; Song, Xuqin; Liu, Qingyong; Zhang, Yuman; He, Lili

doi:10.1016/j.jchromb.2015.03.009

Cited by 26 publications

(21 citation statements)

References 18 publications

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“…Based on the above point, some kinds of solvents are widely used, such as dichloromethane, acetonitrile, methanol, acetone, and water. [324][325][326][327][328] Eluting the target analytes from MIP sorbents using a specific solvent is the last step of MISPE. The eluting solvent should be strong enough to release the analytes from the binding sites.…”

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confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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a Molecular imprinting technology (MIT), often described as a method of making a molecular lock to match a molecular key, is a technique for the creation of molecularly imprinted polymers (MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Owing to their unique features of structure predictability, recognition specificity and application universality, MIPs have found a wide range of applications in various fields. Herein, we propose to comprehensively review the recent advances in molecular imprinting including versatile perspectives and applications, concerning novel preparation technologies and strategies of MIT, and highlight the applications of MIPs. The fundamentals of MIPs involving essential elements, preparation procedures and characterization methods are briefly outlined.Smart MIT for MIPs is especially highlighted including ingenious MIT (surface imprinting, nanoimprinting, etc.), special strategies of MIT (dummy imprinting, segment imprinting, etc.) and stimuli-responsive MIT (single/dual/ multi-responsive technology). By virtue of smart MIT, new formatted MIPs gain popularity for versatile applications, including sample pretreatment/chromatographic separation (solid phase extraction, monolithic column chromatography, etc.) and chemical/biological sensing (electrochemical sensing, fluorescence sensing, etc.). Finally, we propose the remaining challenges and future perspectives to accelerate the development of MIT, and to utilize it for further developing versatile MIPs with a wide range of applications (650 references).

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Molecular imprinting: perspectives and applications

et al. 2016

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“…CPH has a similar effect on animals as clenbuterol, thus it has been utilized as a feed additive. Currently, in consideration of the allergic and other potential hazards for consumers posed by CPH residues in animal edible tissues [5], CPH has been banned as a veterinary drug for food-producing animals according to EU Directive 2001/82 [6]. In the announcement No.…”

Section: Introductionmentioning

confidence: 99%

“…176 and 1519 of China's Ministry of Agriculture, CPH was prohibited to use in feedstuffs and drinking water [7]. However, many investigations have shown illegal use or overuse of CPH by farmers to promote growth of animals due to its price advantage and effectiveness [6,8]. It follows that an available test tool is urgently needed to monitor the illegal use of CPH.…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Na¹,

Hu²,

Sun³

et al. 2020

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Background: An antihistamine cyproheptadine (CPH) and phenothiazines (PZs)sedative hypnoticshave a similar tricyclic structure, these drugs are often illegally added to food animal feedstuffs due to their price advantage and significant effect in promoting growth and improving meat quality. However, the abuse of these drugs may lead totheir residues in animal products, thereby causing harmful effects such as allergies and dermatological reactions on human health.To supervise the use of prohibited drugs and ensure food safety, it is necessary to establish a simple and effective screening method to detect CPH and PZs.In this research, an artificial antigen against cyproheptadine(CPH) was successfully synthesized by bromoacetic acid substitution method. An ultrasensitive and broad-specific monoclonal antibody (mAb) able to recognize CPH and six phenothiazines (PZs) was produced. Based on the gold-labeled mAb, an immunochromatographic strip (ICS) was established.Results:The 50% inhibition concentration (IC50) of the produced mAb against CPH was identified as 0.036 ng mL-1 by ELISA, and the cross-reactivities for six PZs were from 6.33% to 63.16%. The visual detection limits(cut-off values) of the developed ICS ranged from 5 to 100ngg-1 in feedstuffs. Take a reading by strip reader, the IC50 was from 0.570 to 7.750ng g-1. In addition,a recovery experiment was carried out to verify the reliability of the ICS. The intra-assay recoveries were from 79.83% to 103.38% with the highest coefficient of variation (CV) of 11.31%. The inter-assay recoveries were from 79.00% to 96.63% with the highest CV of 12.66%.Conclusions: We have successfully produced a broad-spectrum monoclonal antibody and established an ICS for simultaneouslydetecting CPH and six PZs drugs.In brief, the proposed ICS was considered suitable for qualitatively and quantitatively monitoring CPH and PZs in feedstuffs.

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“…Molecular imprinting is regarded as a promising technique for preparing artificial receptors with “tailor‐made”‐binding sites for specific binding the template molecular by polymerizing a mixture of appropriate functional monomer, befitting cross‐linker and template . Molecularly imprinted polymers (MIPs) have been successfully applied in the fields of SPE , sensors , and drug delivery/release because of their eminent merits involving mechanical and chemical stability, predetermined recognition ability, easy preparation, and high tolerance for high temperature or pH. To date, this auspicious technique has been mature to imprint low‐weight molecules , while it's still a challenge to imprint macromolecules like proteins for its large molecular size, conformational flexibility, water solubility, and complexity.…”

Section: Introductionmentioning

confidence: 99%

Selective adsorption of protein by a high-efficiency Cu²⁺-cooperated magnetic imprinted nanomaterial

et al. 2016

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We report a core-shell magnetic molecularly imprinted polymer with high affinity through a facile sol-gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption-desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics.

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Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC

Cited by 26 publications

References 18 publications

Molecular imprinting: perspectives and applications

Molecular imprinting: perspectives and applications

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Selective adsorption of protein by a high-efficiency Cu²⁺-cooperated magnetic imprinted nanomaterial

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Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC

Cited by 26 publications

References 18 publications

Molecular imprinting: perspectives and applications

Molecular imprinting: perspectives and applications

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Selective adsorption of protein by a high-efficiency Cu2+-cooperated magnetic imprinted nanomaterial

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Selective adsorption of protein by a high-efficiency Cu²⁺-cooperated magnetic imprinted nanomaterial