Determination of Cyazofamid and Its Metabolite in Oily Agricultural Products with HPLC-MS/MS

Abstract

A high-performance liquid chromatography-tandem mass spectrometry method was developed for the determination of cyazofamid and its major metabolite 4-Chloro-5-(4-tolyl)-1H-imidazole-2-carbonitrile (CCIM) in oily samples. Samples were extracted with acetonitrile, which contained 1% acetic acid and cleaned-up with C18 and Florisil absorbents. Recoveries ranged from 75.91% to 109.85% with coefficients of variation from 5.14% to 10.69%. The limits of detection (LODs) and the limits of quantification (LOQs) were >0.0020 mg kg−1 and ≤0.0040 mg·kg−1, respectively, which were smaller than maximum residue levels established by Australia for oily samples. The proposed fragmentation pathway of cyazofamid and CCIM were discussed.

Design, Synthesis and Bioactivity Evaluation of Novel 2-(pyrazol-4-yl)-1,3,4-oxadiazoles Containing an Imidazole Fragment as Antibacterial Agents

“…Encouragingly, a number of imidazole core-containing medicines and pesticides have been successfully developed, especially in agricultural fields, and imidazole derivatives are widely applied as fungicides, herbicides and insecticides ( Figure 1 ). For example, prochloraz, carbendazim, imazalil, thiabendazole, triflumizole, cyazofamid and pefurazoate are used to control plant fungal diseases [ 12 , 13 , 14 , 15 , 16 , 17 ], and some of them are also used as fungicides in coatings, synthetic resins, paper products, metal products and household appliances [ 18 ]. Meanwhile, dimetridazole and parbendazole are veterinary vermifuges and growth-promoting agents in the breeding industry, as well as feed additives for pigs and chickens [ 19 , 20 , 21 ].…”