Determination of cucurbitacin E in some selected herbs of ayurvedic importance through RP-HPLC

Abstract

Background The consumption of the fruits of cucurbitaceae plants is widely popular among Indians due to their various nutritional and medicinal purposes. Some of these plants are well reported in Ayurveda due to their potential therapeutic importance. In particular, the plants of this family are well-characterized by the presence of its bitter principle, Cucurbitacin E which differs within the species due to its genetic variations. Objectives The objective of the study was to develop a validated RP-HPLC method for standardization in some widely consumed cucurbits with cucurbitacin E as a marker compound. Materials and methods The RP-HPLC method was developed with a reverse phase C 18 column, using acetonitrile and water (1% glacial acetic acid) as mobile phase (70:30 v/v). The flow rate and λ max were optimized at 1 mL/min and 230 nm respectively. The HPLC method was validated in terms of accuracy, specificity, sensitivity, and repeatability as per ICH guideline. Results The calibration curve was found linear in the concentration range of 1–100 μg/mL. The % RSD of precision and recovery was found to be <2%, which confirms high repeatability of the method. The results indicated that the content of cucurbitacin E was highest (0.0663% w/w) in Cucurbita pepo whereas Lagenaria siceraria contains the lowest (0.0356% w/w). Conclusion The study was able to explore the variation of cucurbitacin E content in some selected food plants of Cucurbitaceae family. The applicability of the method can be established in nutraceutical industry for the effective quality control of cucurbits for safe human consumption.

Progress on Genetic Improvement and Analysis of Bottle Gourd [Lagenaria siceraria (Molina) Standl.] for Agronomic Traits, Nutrient Compositions, and Stress Tolerance: A Review

“…The contents of cucubitacins type E and I have been quantified in leaves (Mashilo et al, 2018;Chanda et al, 2019). The concentration of cucurbitacin I was 0.04%w/w in mature fruit (Chanda et al, 2019). Cucurbitacin I contents of 1.00, 6.34, and 1.61 mg/g were reported in the fruit epicarp, fruit mesocarp, and seeds of wild bottle gourd, in that order (Attar and Ghane, 2018).…”

“…The leaves and fruits are rich sources of bio-active compounds such as cucurbitacins Ghane, 2018, 2019;Chanda et al, 2019) which have various health benefits such as antioxidant, antidiabetic, anti-tumor, anti-proliferrative and antimicrobial properties (Marzouk et al, 2012;Ma et al, 2014;Ku et al, 2017;Attar and Ghane, 2019). The contents of cucubitacins type E and I have been quantified in leaves (Mashilo et al, 2018;Chanda et al, 2019). The concentration of cucurbitacin I was 0.04%w/w in mature fruit (Chanda et al, 2019).…”

Phytochemical and Nutritional Studies of Bottle Gourd (Lagenaria Siceraria Ls) Cultivated in Egyptian Habitats

“…The plant includes about 118 genera and 825 species, which are extensivelyspread in the hotter regions of the World [3][4][5] . Ethnobotanical,epidemiological,and traditional information related to the medicinal and nutritional capacity of bottle gourd has been extensively reviewed [6][7][8][9][10][11][12] . Cucurbits (family Cucurbitaceae) seeds have been reported as strong sources of food, particularly protein and oil, where dehulled seeds of cucurbit were reported to have about 50% oil and up to 35 % protein [13,14] .…”

Identification and Quantification of Key Phytochemicals, Phytohormones, and Antioxidant Properties in Coccinia grandis during Fruit Ripening

“…In addition, the content of cucurbitacin D was highest in the GRS, suggesting that the immature C. grandis fruit has pharmacological potential and has potential as a source of functional foods and therapeutic agents. Previous studies have reported that cucurbitacin E was found in C. grandis fruits [ 44 ]. However, in this study, cucurbitacin B was found at all stages, cucurbitacin D was identified in the GRS and HRS, and cucurbitacin I was found in trace amounts in the HRS.…”