Determination of crystal orientation from micrographs using a MATLAB program

Gibson, Ursula J.; Kou, Yi

doi:10.1107/s0021889805007909

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…A halfwave plate in the beam path could be used to adjust the direction of the plane of polarization of the trapping beam. The exact alignment of the individual particles in the trap could be determined using a software package developed by Gibson and Kou [17].…”

Section: Orientation Of Lysozyme Crystalsmentioning

confidence: 99%

Orientation of optically trapped nonspherical birefringent particles

et al. 2006

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While the alignment and rotation of microparticles in optical traps have received increased attention recently, one of the earliest examples has been almost totally neglected-the alignment of particles relative to the beam axis, as opposed to about the beam axis. However, since the alignment torques determine how particles align in a trap, they are directly relevant to practical applications. Lysozyme crystals are an ideal model system to study factors determining the orientation of nonspherical birefringent particles in a trap. Both their size and their aspect ratio can be controlled by the growth parameters, and their regular shape makes computational modeling feasible. We show that both external ͑shape͒ and internal ͑birefringence͒ anisotropy contribute to the alignment torque. Three-dimensionally trapped elongated objects either align with their long axis parallel or perpendicular to the beam axis depending on their size. The shape-dependent torque can exceed the torque due to birefringence, and can align negative uniaxial particles with their optic axis parallel to the electric field, allowing an application of optical torque about the beam axis.

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Section: Orientation Of Lysozyme Crystalsmentioning

confidence: 99%

Orientation of optically trapped nonspherical birefringent particles

et al. 2006

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“…A graphical user interface permits matching of the cage to the underlying image, and returns the volume of the crystal and orientation angle of the c-axis with respect to the axis of the trapping beam 10 . …”

Section: Lysozyme Crystalsmentioning

confidence: 99%

Growth of crystals in optical tweezers

et al. 2005

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We report here on the use of optical tweezers in the growth and manipulation of protein and inorganic crystals. Sodium chloride and hen egg-white lysozyme crystals were grown in a batch process, and then seeds from the solution were introduced into the optical tweezers. The regular and controllable shape and the known optical birefringence in these structures allowed a detailed study of the orientation effects in the beam due to both polarization and gradient forces. Additionally, we determined that the laser tweezers could be used to suspend a crystal for three-dimensional growth under varying conditions. Studies included increasing the protein concentration, thermal cycling, and a diffusioninduced increase in precipitant concentration. Preliminary studies on the use of the tweezers to create a localized seed for growth from polyethylene oxide solutions are also reported.

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Crystal morphology study of N,N′-diacetylchitobiose by molecular dynamics simulation

Yang

Dong

2011

Carbohydrate Research

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Determination of crystal orientation from micrographs using a MATLAB program

Cited by 4 publications

References 8 publications

Orientation of optically trapped nonspherical birefringent particles

Orientation of optically trapped nonspherical birefringent particles

Growth of crystals in optical tweezers

Crystal morphology study of N,N′-diacetylchitobiose by molecular dynamics simulation

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