Determination of copper in composite biomaterials by capillary electrophoresis: an UV-direct method based on in situ complex formation

Abstract

This work describes the optimization and validation of an UV-direct capillary electrophoresis method for the analysis of copper ions in samples obtained from release studies of bone-tissue-engineering composite biomaterials.

Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: copper and strontium

“…The Cu 2+ release from the films was studied in phosphate buffer (10 mM, pH 7.4) at 37 1C according to a technique previously reported. 40 Aliquots were withdrawn from the media at regular intervals (1, 3 and 6 hours and 1, 3, 7 and 14 days) and the films were re-immersed in fresh buffer at each collection time. Quantification was made by capillary electrophoresis: 40 this method combines the in situ complexation of free copper ions with ethylenediaminetetraacetic acid (EDTA) in the electrophoresis column (reverse polarity, acidic conditions) to the subsequent detection and quantification of the complexes by UV radiation.…”

Efficient UPLC and CE methods for the simultaneous determination of azelastine hydrochloride and its genotoxic impurity

“…In the same way, CE has broad application since it is time and cost effective. In addition, aqueous solvents are frequently used in CE rather than organic ones and hence CE is considered an ecofriendly method (Cattalini, Mouriño, & Lucangioli, ).…”

Square Wave‐Anodic Stripping Voltammetric Determination of Copper at a Bismuth Film/Glassy Carbon Electrode Using 3‐[(2‐Mercapto‐Vinyl)‐Hydrazono]‐ 1,3‐Dihydro‐Indol‐2‐One

“…It includes, time consuming processes, preconcentration steps, costly solvents and expensive instrumentation, expertise for its proper operation and complexity . Thus, great attention for analysis of copper has been focused towards constructing simple system with short analytical time, good reproducibility, accuracy, selectivity, ruggedness and cost‐effectiveness .…”

“…Surface modified electrodes (SMEs) are known to be one of the most suitable alternatives to mercury electrodes. SMEs coupled stripping methods is a highly comprehensive approach for addressing shortcomings with current methodologies for trace analysis . The most common practice utilized is the enhancement of selectivity towards host and target .…”

“…SMEs coupled stripping methods is a highly comprehensive approach for addressing shortcomings with current methodologies for trace analysis . The most common practice utilized is the enhancement of selectivity towards host and target . Based on the principle, electrode modification by a series of reagents including amoxicillin , hexacyanoferrate , tetraethylthiuram disulfide , tetraphenylporphyrin , p‐tert‐butylcalix(4)arene , organofunctionalized silica and nafion have been reported for copper determination.…”