Determination of coplanar polychlorobiphenyl congeners in biota samples

Pastor, María De Miguel; Sanchez, J.; Barceló, Damià; Albaigés, Joan

doi:10.1016/0021-9673(93)87047-p

Cited by 23 publications

(3 citation statements)

References 19 publications

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“…temperature was 50°C) and then taken up in hexane. The cleanup was carried out by concentrated sulfuric acid in the fat fraction previously dissolved in hexane, following the procedure described by Pastor et al (1993). After centrifugation, the organic phase was passed through a sodium sulfate anhydrous column, rinsed with hexane, concentrated under a flow of nitrogen, and stored at 4°C before analyzing by gas chromatography-mass spectrometry (GC-MS).…”

Section: Methodsmentioning

confidence: 99%

Polychlorinated biphenyls and organochlorine pesticide levels in tissues of Caretta caretta from the Adriatic Sea

Perugini

Giammarino²,

Olivieri³

et al. 2006

Dis. Aquat. Org.

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We detected concentrations of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs) in the liver, muscle, and fat of 11 loggerhead sea turtles Caretta caretta from the central and southern Adriatic Sea. All samples contained PCBs at various concentrations, with Congener 138 (28%), 153 (27%), and 180 (32%) dominating the congener composition of the tissues. The dioxin-like congener (118, 13%) was detected in all tissues analyzed. The lower-chlorinated PCBs were not detected. The average of the total PCB concentrations, expressed in nanograms per gram wet weight, was 459.6 ng g -1 in fat, 82.9 ng g -1 in liver, and 5.8 ng g -1 in muscle. Among 13 organochlorine pesticides for which analyses were conducted, 4 were detected: p,p '-DDE (57%); p,p '-DDD (16%); and p,p '-DDT and o,p '-DDT (27%). Spatial differences were found among OC concentrations in loggerheads from the central and southern Adriatic Sea. The only samples containing detectable concentrations of p,p '-DDT and o,p '-DDT were from the southern area.

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Section: Methodsmentioning

confidence: 99%

Polychlorinated biphenyls and organochlorine pesticide levels in tissues of Caretta caretta from the Adriatic Sea

Perugini

Giammarino²,

Olivieri³

et al. 2006

Dis. Aquat. Org.

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“…The lipid content was determined gravimetrically from a five-milliliter aliquot of the extract while the remaining extract was rotary evaporated at 40 o C to dryness. The residue was dissolved in 2 ml n-hexane and the fat removed by treatment with concentrated sulphuric acid (saturated with n-hexane) as described by Bergen et al (1993) and Pastor et al (1993). The clear organic extract was cleaned-up by passing it through a silica gel column with anhydrous sodium sulfate placed at the top as described by Perugini et al (2004).…”

Section: Materials and Methods Sampling Sample Preparation And Analysismentioning

confidence: 99%

Organochlorine Pesticide Residues in Sardinella Aurita from the Coastal Waters of Accra-Tema, Ghana and their Potential Health Risks

Nyarko¹,

Botwe²,

Bampoe³

et al. 2011

Journal of the Ghana Science Association

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Sardinella aurita and seawater samples from the Accra-Tema coast of Ghana were analysed for organochlorine (OC) pesticides using gas chromatography (GC) with electron capture detection (ECD). Residues of DDTs (p,p'-DDT, p,p'-DDE, p,p'-DDD), hexachlorocyclohexane (HCHs-α-HCH, β-HCH and γ-HCH), endosulfan (α-endosulfan, β-endosulfan, endosulfan sulphate) and heptachlor were detected in fish at average concentrations ranging from 3.0 to 1235.0 μg/kg wet wt. These residues were, however, below the detection limit in seawater samples. Significant differences (p < 0.05) in residue levels were also observed across sites, decreasing from Chorkor to Kpone for seven (7) of the residues that were detected in S. aurita. Evaluation of hazard ratios (HRs) and the health risk associated with OC exposure from consumption of sardinellas revealed a probability of adverse health effect due to p,p'-DDT, γ-HCH and heptachlor. Hence, p,p'-DDT, γ-HCH and heptachlor may be of particular health concern.

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“…Chromatographic peaks of a non-ortho substituted congener (PCB 126) eluted with dichloromethane at different column temperatures: 20 ~ 40 ~ and 60 ~ back-flushing requirement [25], high consumption of elution solvent [24,26] and low chromatographic performance for the most highly retained planar PCBs [29][30][31][32].…”

Section: Figurementioning

confidence: 99%

Temperature effect on HPLC retention of PCBs on porous graphitic carbon

2000

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KeyWordsColumn liquid chromatography Porous graphitic carbon Temperature effect on retention Polychlorobyphenyls SummaryThe effect of column temperature on solute retention was investigated for 10 PCBs with different degrees of planarity (degree of ortho position substitution). The variation of retention values (ln k) with temperature was measured using two solvents, n-hexane and dichloromethane, as mobile phase: retention decreases as temperature increases. The relationship between Ink values and temperature (l/T) exhibits a good linearity. The AH ~ values calculated are in the -2 to -5 kcal mole-1 range: they are very similar for compounds with the same degree of ortho-substitution and are significantly higher for non-ortho substituted congeners. High temperature significantly improves separation efficiency (plate number significantly increases): this effect is the dominant factor controlling chromatographic resolution, which improves with increasing temperature. Operating at 40 ~ an efficient separation of planar PCBs was obtained with low solvent consumption.

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Determination of coplanar polychlorobiphenyl congeners in biota samples

Cited by 23 publications

References 19 publications

Polychlorinated biphenyls and organochlorine pesticide levels in tissues of Caretta caretta from the Adriatic Sea

Polychlorinated biphenyls and organochlorine pesticide levels in tissues of Caretta caretta from the Adriatic Sea

Organochlorine Pesticide Residues in Sardinella Aurita from the Coastal Waters of Accra-Tema, Ghana and their Potential Health Risks

Temperature effect on HPLC retention of PCBs on porous graphitic carbon

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