Determination of colistin in animal tissues, egg, milk, and feed by ultra-high performance liquid chromatography-tandem mass spectrometry

Fu, Qin; Li, Xiaowei; Zheng, Kangni; Ke, Yuebin; Wang, Yingyu; Wang, Lina; Yu, Fugen; Xia, Xi

doi:10.1016/j.foodchem.2017.12.029

Cited by 36 publications

(32 citation statements)

References 25 publications

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“…Solid-phase extraction (SPE) technique has been deemed as the most suitable alternative for the analysis of water samples. To date, SPE has not been used to concentrate polymyxins from water, yet, several reports introduced HLB, WCX and C 18 sorbents to extract polymyxins from animal foodstuff [16,17], feed [18,19] and plasma [20]. Unfortunately, traditional SPE sorbents are not selective and thus result in strong matrix effects [2,17,21].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Molecularly Imprinted Polymers for the Selective Extraction of Polymyxins from Environmental Water Samples

Song

Turiel

et al. 2020

Polymers

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The emergence of colistin resistance gen has aroused public concern. It is significant to assess the concentrations of polymyxins residues in aquatic environment since resistant bacteria carrying colistin resistance gen are frequently isolated from wastewater; surface water and ground water. However; no literature on the determination of polymyxins in water is available; probably due to the absence of an efficient extraction method. Accordingly; molecularly imprinted polymers were synthesized by precipitation polymerization with colistin as the template. The polymers were successfully used as sorbents for the determination of polymyxins from water based on molecularly imprinted solid-phase extraction and high-performance liquid chromatography–ultraviolet detection. The molecularly imprinted cartridge showed excellent affinity and cross-reactivity to analytes in aqueous media. Recoveries obtained from water samples were between 65.9% and 90.1%, with relative standard deviations lower than 10.2%. Limits of detection were between 1.0 and 2.0 μg L−1 concentration levels. Compared with C18 cartridge; the molecularly imprinted cartridge could remove more interference from co-extracted matrices. This method is practical for the routine monitoring of polymyxin residues in environmental water; which will benefit studies on drug-resistance and occurrence of polymyxins in the environment.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Molecularly Imprinted Polymers for the Selective Extraction of Polymyxins from Environmental Water Samples

Song

Turiel

et al. 2020

Polymers

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“…The most common issues encountered during peptide chromatography are peak shape, separation, and carryover. In order to obtain sharp and symmetrical peaks on a C18 column, acidified mobile phases with different concentrations of formic acid [ 9 , 10 , 11 , 12 , 15 ] and/or trifluoroacetic acid were used [ 14 ]. The advantage of low pH is the suppression of ionization of silanol groups, thereby limiting secondary interaction with cationic compounds and thus preventing peak tailing.…”

Section: Resultsmentioning

confidence: 99%

“…Potential column contamination was prevented through the use of the mobile phase gradient; although the retention time of the polypeptide was <2.20 min, the gradient continued for 6 min, to ensure that all elutable components were cleared and the system was re-equilibrated prior to injecting the next sample. Unlike other LC-MS/MS methods [ 8 , 9 , 11 , 14 , 15 ], the time of chromatographic analysis is significantly shorter and comparable to those in which only colistins were determined [ 10 , 12 ]. Blank samples were also run to ensure that no carryover or matrix effects were present ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…The sample preparation procedure principally involved a deproteinization step (referred to as acidic pretreatment), which is performed by organic solvents (methanol and acetonitrile) and acids (trifluoroacetic acid, trichloroacetic acid, and hydrochloric acid) in different combinations [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. After that step, solid-phase extraction is implemented on polymeric-based SPE [ 9 , 10 , 11 , 14 ], C18-SPE [ 15 ], or ion exchange cartridges [ 12 , 13 ]. Finally, before LC-MS/MS analysis one of several different filtration steps occurs [ 8 , 10 , 11 , 12 , 15 ].…”

Section: Resultsmentioning

confidence: 99%

“…Xu et al [ 10 ] published a method for colistin A and B in fishery products, and Zhang et al [ 11 ] developed a method for the determination of bacitracin and polymyxin B in livestock products. Recently, two methods were developed for the determination of colistin A and B in animal tissues [ 12 , 13 ]. Meanwhile, there are only a few multi-residue methods for the determination of polypeptide antibiotics in animal matrices.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

2020

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A novel UHPLC-MS/MS method for the determination of polypeptide antibiotic residues in animal muscle, milk, and eggs was developed and validated. Bacitracin A, colistin A, colistin B, polymyxin B1, and polymyxin B2 were extracted from the samples with a mixture of acetonitrile/water/ammonia solution 25%, 80/10/10 (v/v/v), and put through further evaporation, reconstitution, and filtration steps. The chromatographic separation was performed on a C18 column in gradient elution mode. Mass spectral acquisitions were performed in selective multiple reaction monitoring mode by a triple quadrupole mass spectrometer. The method was validated according to the criteria of Commission Decision 2002/657/EC. The method quantifies polypeptides in a linear range from 10 to 1000 μg kg−1, where the lowest concentration on the calibration curve refers to the limit of quantification (LOQ). The recoveries ranged from 70 to 99%, the repeatability was below 13%, and within-laboratory reproducibility was lower than 15%. The decision limit (CCα) and detection capability (CCβ) values were calculated, and ruggedness and stability studies were performed, to fulfill the criteria for confirmatory methods. Moreover, the developed method may also be used for screening purposes by its labor efficiency.

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Identification and absolute quantification of animal blood products by peptide markers using an UPLC–MS/MS method

Zhang

Wang

et al. 2020

Eur Food Res Technol

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Determination of colistin in animal tissues, egg, milk, and feed by ultra-high performance liquid chromatography-tandem mass spectrometry

Cited by 36 publications

References 25 publications

Synthesis of Molecularly Imprinted Polymers for the Selective Extraction of Polymyxins from Environmental Water Samples

Synthesis of Molecularly Imprinted Polymers for the Selective Extraction of Polymyxins from Environmental Water Samples

Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Identification and absolute quantification of animal blood products by peptide markers using an UPLC–MS/MS method

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