Determination of colchicine and colchiceine in microbial cultures by high-performance liquid chromatography

Klein, A. E.; Davis, Patrick J.

doi:10.1021/ac50064a048

Cited by 26 publications

(11 citation statements)

References 28 publications

(24 reference statements)

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“…Similarly, HPLC analysis also showed two different peaks at 2.5 min (for 3-DMC) and 3.5 min (for colchicine) retention times and obtained data supported the previous findings [22] (Supporting Information). Final confirmation of 3-DMC with 13 C-NMR chromatogram (Fig.…”

Section: Separation Of Mixture Of Colchicine and 3-dmc In A Chromatogsupporting

confidence: 89%

Enhanced extraction of 3‐demethylated colchicine from fermentation broth of Bacillus megaterium: Optimization of process parameters by statistical experimental design

Dubey¹,

Jawed

Haque

2011

Engineering in Life Sciences

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Biotransformation of colchicine into its pharmacologically active regiospecific derivative 3‐demethylated colchicine (3‐DMC) mediated by microbial monooxygenases is an economic and promising strategy for the production of this inexpensive and potent anti‐cancer drug. Response surface methodology (RSM), with central composite design (CCD) model, was employed with three extraction variables (temperature, pH, and process time) for optimization; which most significantly affected recovery of 3‐DMC. The validity of the model was ascertained by running the predicted values in an individual run; and the optimum parameters, temperature (50°C), pH (10), and process time (120 min) resulted in a maximum recovery of 3‐DMC 4128 mg/L when chloroform (1% v/v) was used as an extraction solvent. RSM in our experiments showed to be a good tool for the extraction of 3‐DMC from the fermentation medium.

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Section: Separation Of Mixture Of Colchicine and 3-dmc In A Chromatogsupporting

confidence: 89%

Enhanced extraction of 3‐demethylated colchicine from fermentation broth of Bacillus megaterium: Optimization of process parameters by statistical experimental design

Dubey¹,

Jawed

Haque

2011

Engineering in Life Sciences

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“…Many studies have dealt with the development of analytical methods for the determination of colchicine, based on TLC-densitometry [1,2], high performance liquid chromatography using UV [1][2][3][4][5][6], or MS detection [7][8][9]. Comparatively, only a few papers have dealt with the electrochemical behavior and dosage of colchicine [10][11][12][13][14], and none of them reflected application of the quantitative determination of the alkaloid from pharmaceutical products.…”

Section: Figmentioning

confidence: 99%

Method validation in quantitative electrochemical analysis of colchicine using glassy carbon electrode

2007

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A cathodic differential pulse voltammetric determination of colchicine was validated using a glassy carbon electrode in HClO 4 /H 3 PO 4 0.01 M. Colchicine gives an irreversible, diffusion-controlled peak at −862 mV vs. Ag/AgCl reference electrode. The cathodic peak is strongly influenced by a more alkaline environment with a shift towards more negative potentials. Method optimization was carried out in parallel for three types of electrodes (glassy carbon, mercury film and bismuth film coated glassy carbon). The cathodic peak current is higher using film-coated electrodes, but shows poorer intra-day reproducibility and a longer analysis time due to film renewal. Thus, a bare glassy carbon electrode was used to determine colchicine in the concentration range of 2.4 − 50 µg mL −1 (R 2 = 0.9998, n = 5), with a calculated detection limit of 0.80 µg mL −1 . The proposed method was characterized according to ICH Harmonized Tripartite Guidance Q2(R1) by validation parameters (selectivity, linearity, accuracy, fidelity, limit of detection, limit of quantification) and it was successfully applied for the determination of colchicine from tablets, without the interference of the excipients. The method's performances were evaluated and compared with both a known polarographic method and the official quantitative spectrophotometric determination from the Romanian Pharmacopoeia, Xth edition, respectively.

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“…Colchiceine can be present in plant extracts besides colchicine, or can appear as a degradation product on storage of 1. [8][9][10][11] Colchiceine can easily form complexes with most of silica based phases, which can be explained by the properties of its ring system especially ring C with a hydroxyl group at C-10 position and neighbouring carbonyl group at carbon atom C-9. It is difficult to detect and separate colchiceine from plant extracts because of its chelating properties.…”

Section: Introductionmentioning

confidence: 99%

Colchiceine Complexes with Lithium, Sodium and Potassium Salts − Spectroscopic Studies

Kurek¹,

Barczyński²

2016

Croat. Chem. Acta

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Colchiceine complexes with Li + , Na + and K + cations have been synthesized and studied by 1 H and 13 C NMR, FT-IR, FAB MS and UV-Vis. It has been shown that colchiceine forms stable complexes especially with lithium cation and the most stable structures of the complexes are those in which the acetamide groups are involved in the coordination process. The structures of the colchiceine complexes with Li + , Na + and K + cations are discussed in details.

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Determination of colchicine and colchiceine in microbial cultures by high-performance liquid chromatography

Cited by 26 publications

References 28 publications

Enhanced extraction of 3‐demethylated colchicine from fermentation broth of Bacillus megaterium: Optimization of process parameters by statistical experimental design

Enhanced extraction of 3‐demethylated colchicine from fermentation broth of Bacillus megaterium: Optimization of process parameters by statistical experimental design

Method validation in quantitative electrochemical analysis of colchicine using glassy carbon electrode

Colchiceine Complexes with Lithium, Sodium and Potassium Salts − Spectroscopic Studies

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