Determination of clindamycin in plasma or serum by high-performance liquid chromatography with ultraviolet detection

Follette, Greg La; Gambertoglio, John G.; White, Jeri A.; Knuth, Dean W.; Lin, Emil T.

doi:10.1016/s0378-4347(00)83106-0

Cited by 24 publications

(18 citation statements)

References 5 publications

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“…1 Procedures describing the determination of clindamycin in biological fluids, using GLC mass spectrometry 2,3 or HPLC-UV detection, 4 are either time-consuming or not very sensitive. 5,6 GLC mass spectrometry requires derivatisation of the sample, which takes at least 60 min. Liu et al 4 used solid-phase extraction prior to a quite long HPLC run.…”

mentioning

confidence: 99%

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard

Rechberger

Fauler

Windischhofer

et al. 2002

Rapid Comm Mass Spectrometry

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A new method for the quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) is presented. Recently published methods possess a disadvantage because of their use of internal standards with extraction and ionisation properties different from those of clindamycin. To avoid these problems, d(1)-N-ethylclindamycin was synthesised for use as internal standard by N-demethylation and subsequent d(1)-N-ethylation. Plasma sample preparation was done by an easy and rapid liquid-liquid extraction using ethyl acetate. The method was validated in the expected concentration range for a pharmacokinetic study. Calibration graphs were linear within the range 0.05-3.2 microg/mL plasma. Intra-day precision was between 0.90% (2.8 microg/mL) and 3.25% (0.05 microg/mL), inter-day variability was found to be between 1.33% (0.7 microg/mL) and 2.60% (0.05 microg/mL). Inter-day accuracy showed deviations between 0.4% (0.05 microg/mL) and -4.8% (0.2 microg/mL). The method is simple and robust, and has been applied to the batch analysis of clindamycin during a pharmacokinetic study.

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confidence: 99%

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard

Rechberger

Fauler

Windischhofer

et al. 2002

Rapid Comm Mass Spectrometry

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“…It is synthesized from microbially fermented lincomycin by replacing a hydroxyl group at the 7-position of lincomycin by a chlorine group, which significantly increases its activity (1). Clindamycin is highly effective against Gram-positive and Gramnegative anaerobic pathogens, as well as Gram-positive aerobes, and appears to inhibit protein synthesis in susceptible organisms by binding 50 S ribosomal subunits; the primary effect is inhibition of peptide bond formation (2,3). Clindamycin is mainly used in the treatment of serious respiratory tract infection, serious skin and soft tissue infections, septicaemia, intra-abdominal infections and infections of the female pelvis and genital tract caused by susceptible anaerobic bacteria (3,4).…”

Section: Clindamycin [7(s)-chloro-7-deoxylincomycin] Is An Antibioticmentioning

confidence: 99%

Ultrasensitive assay of clindamycin in medicine and bio‐fluids with chemiluminescence detection

Wei

Dang

2007

Luminescence

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A simple chemiluminescence (CL) method using flow injection has been developed for the determination of clindamycin, based on the inhibitory effect of clindamycin on the CL generated from the luminol-K(3)Fe(CN)(6) system in alkaline medium. It was found that the decrement of CL intensity was linear with the logarithm of clindamycin concentration over the range 0.7-1000 ng/mL. The detection limit was 0.2 ng/mL with a relative standard deviation (RSD) of <5.0% (n = 7). At a flow rate of 3.0 mL/min, a complete analytical process could be performed within 0.5 min, including sampling and washing. The proposed procedure was applied successfully to the determination of clindamycin in pharmaceutical preparations and human urine without pretreatment.

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“…It is an antibiotic that is highly effective against Gram-positive and Gram-negative anaerobic pathogens, as well as Gram-positive aerobes. 1 It appears to inhibit protein synthesis in susceptible organisms by binding 50S ribosomal subunits and its primary effect is the inhibition of peptide bond formation. Because its antibacterial activity is 4-8 times higher than that of lincomycin and it has lower side effect, it is more widely used in the clinical practice and can be utilized for the treatment of serious respiratory tract infections, serious skin and soft tissue infections, septicemia, genital tract infection, etc.…”

Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh Scattering Spectrum of Palladium(II)‐ Clindamycin‐Halofluorescein Systems and Their Analytical Applications

Liu

et al. 2008

Chin. J. Chem.

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In pH 5.0-5.4 HOAc-NaOAc buffer solution, clindamycin (Clin) could react with Pd(II) to form a 1∶1 cationic chelate, which could further react with halofluorescein dyes such as diiodofluorescein (DIF), erythrosine (Ery) and eosin Y (EY) to form 1∶1 ion-association complexes. As a result, not only the absorption and fluorescence spectra were changed, but also the resonance Rayleigh scattering (RRS) intensities enhanced greatly and new RRS spectra appeared. The three reaction products had characteristic RRS spectra and their maximum RRS wavelengths were located at 285 (DIF system), 287 (Ery system) and 321 (EY system) nm, respectively. The scattering intensities were proportional to the concentration of Clin in certain range which could be applied to determine Clin. The linear ranges and detection limits of Clin were 0.025-2.1 µg•mL -1 and 7.8 ng•mL -1 for the DIF system, 0.053-2.4 µg•mL -1 and 16.0 ng•mL -1 for the Ery system, 0.038-2.4 µg•mL -1 and 11.0 ng•mL -1 for the EY system, respectively. In this work, the optimum reaction conditions and the foreign substances were investigated. A simple, sensitive and fast method was developed for the determination of Clin either in the pharmaceutical form or in the human body fluid. Moreover, the composition, structure, reaction mechanism of the ternary complexes and their effects on the absorption, fluorescence and RRS spectra as well as the reasons of RRS enhancement were discussed.

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Determination of clindamycin in plasma or serum by high-performance liquid chromatography with ultraviolet detection

Cited by 24 publications

References 5 publications

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard

Ultrasensitive assay of clindamycin in medicine and bio‐fluids with chemiluminescence detection

Resonance Rayleigh Scattering Spectrum of Palladium(II)‐ Clindamycin‐Halofluorescein Systems and Their Analytical Applications

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Determination of clindamycin in plasma or serum by high-performance liquid chromatography with ultraviolet detection

Cited by 24 publications

References 5 publications

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d1‐N‐ethylclindamycin as internal standard

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d1‐N‐ethylclindamycin as internal standard

Ultrasensitive assay of clindamycin in medicine and bio‐fluids with chemiluminescence detection

Resonance Rayleigh Scattering Spectrum of Palladium(II)‐ Clindamycin‐Halofluorescein Systems and Their Analytical Applications

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Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard

Quantitative analysis of clindamycin in human plasma by liquid chromatography/electrospray ionisation tandem mass spectrometry using d₁‐N‐ethylclindamycin as internal standard