Determination of clindamycin in pharmaceuticals by high-performance liquid chromatography using ion-pair formation

A new HPLC/UV method for the determination of clindamycin in dog blood serum

“…Landis et al [8] separated clindamycin from relative compounds in pharmaceuticals on a C 18 analytical column and reported a positive correlation between ion-pair formation and the number of carbons in the alkylsulfonate molecule used as ion-pairing agent. La Follette et al [14] also used tetramethylammonium chloride and adjusted the pH of the mobile phase to 6.7.…”

“…The pH of the aqueous phase was adjusted to 3.5, which is relatively low compared to the pH used by the majority of other investigators [8,10,14,15], although Hornedo-Nuñez et al [9] successfully applied a mobile phase with a pH 2.5. Adjustment was performed with the use of 1 M ortho-phosphoric acid.…”

“…Gas-liquid chromatography (GLC) [5,6] compensates for the previous, but requires relatively complicated sample manipulation. HPLC methods [7][8][9][10] are much more accurate and precise. Refractive index [7,8], electrochemical [9], and UV [8,10] detection have been applied.…”

Development and validation of a gradient HPLC method for the determination of clindamycin and related compounds in a novel tablet formulation

“…[2] was found to be unsuitable for use with the new formulation, because triethyl citrate (a tablet excipient) and degradation product A (an impurity resulting from exposure of clindamycin to one of the excipients, This prompted a review of the literature, which showed that several HPLC methods have been developed over the years for clindamycin and clindamycin-related impurities [1][2][3][4][5][6][7][8]. Some of these relied upon ion-pair reagent in the mobile phase to effect the separation; therefore, they were not considered for this effort because of the inherent instability and long equilibration times often associated with such methods.…”

Separation and characterization of clindamycin and related impurities in bulk drug by high-performance liquid chromatography-electrospray tandem mass spectrometry

“…mentation; 7-epiclindamycin is produced during the synthesis of clindamycin [3]. The rapid identification and characterization of minor impurities is an important task.…”