Determination of cis- and trans-isomers of dosulepine and dithiadene by high-performance liquid chromatography

Šlais, Karel; Subert, J

doi:10.1016/s0021-9673(00)86373-6

Cited by 9 publications

(3 citation statements)

References 9 publications

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“…by non-aqueous titration, which lacks accuracy and sensitivity [2]. The few analytical methods published for its determination include use of potentiometry [3], spectrophotometry [4,5], polarography [6], HPLC [7,8] and GC-MS [9,10].…”

Section: Introductionmentioning

confidence: 99%

Kinetic spectrophotometric methods for the determination of dothiepin hydrochloride in bulk and in drug formulation

Taha

2003

Analytical and Bioanalytical Chemistry

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Two simple and sensitive kinetic methods for the determination of dothiepin hydrochloride are described. The first method is based on kinetic investigation of the oxidation reaction of the drug with alkaline potassium permanganate at room temperature for a fixed time of 25 min. The absorbance of the colored manganate ions is measured at 610 nm. The second method is based on the reaction of dothiepin hydrochloride with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in the presence of 0.1 mol L(-1) sodium bicarbonate. Spectrophotometric measurement was achieved by recording the absorbance at 470 nm for a fixed time of 60 min. All variables affecting the development of the color were investigated and the conditions were optimized. Plots of absorbance against concentration in both procedures were rectilinear over the ranges 4-24 and 50-250 microg mL(-1), with mean recoveries 99.33+/-0.42 and 99.88+/-0.53, respectively. The proposed methods were successfully applied for the determination of dothiepin hydrochloride in bulk powder and in capsule dosage form. The results obtained were found to agree statistically with those given by the non-aqueous B.P. method. Furthermore the methods were validated according to USP guidelines and also assessed by applying the standard addition technique. The determination of dothiepin hydrochloride by the fixed concentration method is feasible with the calibration equations obtained, but the fixed time method proves to be more applicable.

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Section: Introductionmentioning

confidence: 99%

Kinetic spectrophotometric methods for the determination of dothiepin hydrochloride in bulk and in drug formulation

Taha

2003

Analytical and Bioanalytical Chemistry

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“…The drug is official in the British Pharmacopoeia [3] which describes a non--aqueous titration method with potentiometric end point detection for its determination. Several techniques have been reported for the determination of DOTH in pharmaceuticals and include high-performance liquid chromatography (HPLC) [4][5][6][7][8], capillary electrophoresis [9], voltammetry [10], ion-selective electrode potentiometry [11], flow injection potentiometry [12], conductometry [13,14] or spectrofluorimetry [15,16].To the best of our knowledge, seven reports [15--21] on the use of visible spectrophotometry were found in the literature for the determination of DOTH in pharmaceuticals. Abdellatef et al [15] have reported a method based on the condensation of the drug with the mixed anhydrides of malonic and acetic acids at 60 °C.…”

mentioning

confidence: 99%

“…chromatography (HPLC) [4][5][6][7][8], capillary electrophoresis [9], voltammetry [10], ion-selective electrode potentiometry [11], flow injection potentiometry [12], conductometry [13,14] or spectrofluorimetry [15,16].…”

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confidence: 99%

Spectrophotometric determination of dothiepin hydrochloride in pharmaceuticals through ion-pair complexation reaction

Abdulrahman

Basavaiah

2012

CI&CEQ

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Two simple, sensitive and extraction-free spectrophotometric methods are described for the determination of dothiepin hydrochloride (DOTH) both in pure form and in pharmaceutical tablets. The methods are based on ion-pair complex formation between dothiepin base (DOT) and two acidic dyes, namely, bromophenol blue (BPB) or bromocresol green (BCG) with absorption maximum at 425 nm for BPB method or 430 nm for BCG method. Beer’s law is obeyed over the concentration ranges of 1.0-15.0 and 1.0-17.5 μg mL-1 DOT for BPB and BCG methods, respectively. The molar absorptivity values and Sandell’s sensitivity values are reported for both methods. The limits of detection (LOD) and quantification (LOQ) were calculated to be 0.18 and 0.53 μg mL-1 for BPB method, and 0.17 and 0.50 μg mL-1 for BCG method, respectively. The stoichiometry of the complex in either case was found to be 1: 1 and the conditional stability constant (KF) of the complexes has also been calculated. The proposed methods were applied successfully to the determination of DOTH in pure form and in its tablet form with good accuracy and precision. Statistical comparison of the results was performed using Student's t-test and variance ratio F-test at 95% confidence level and there was no significant difference between the official and proposed methods with regard to accuracy and precision. Further, the validity of the proposed methods was confirmed by recovery studies via standard addition technique

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Crystal structure of two tricyclic antidepressant drugs: Lofepramine hydrochloride and dothiepin hydrochloride

Bandoli

Nicolini

Casellato³

1987

Journal of Crystallographic and Spectroscopic Research

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Determination of cis- and trans-isomers of dosulepine and dithiadene by high-performance liquid chromatography

Cited by 9 publications

References 9 publications

Kinetic spectrophotometric methods for the determination of dothiepin hydrochloride in bulk and in drug formulation

Kinetic spectrophotometric methods for the determination of dothiepin hydrochloride in bulk and in drug formulation

Spectrophotometric determination of dothiepin hydrochloride in pharmaceuticals through ion-pair complexation reaction

Crystal structure of two tricyclic antidepressant drugs: Lofepramine hydrochloride and dothiepin hydrochloride

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