Determination of Chlorophyll and Carotenoids Present in the Seeds of the Plant Family Apiaceae and the Impact on Seed Quality

Abstract

In this study, we assessed the content of chlorophyllaandb, β-carotene and lutein in seeds of carrot, celery, dill, parsley and parsnip commercial varieties by spectrophotometric method. It was determined that the content of chlorophyllawas higher than chlorophyllband both differed significantly between plant species and varieties. The highest amounts of chlorophylla, chlorophyllb, β-carotene and lutein were detected in parsley “Olomoucka dlouha”. The lowest amounts of chlorophylla, chlorophyllb, β-carotene and no lutein were detected in celery “Nuget”, however, very low amount of lutein was detected in the parsley cultivars “Atika” and “Orbis”. The content of β-carotene was higher than that of lutein in most varieties. The individual pigment contents were compared to seed quality parameters such as standard laboratory germination and seedling emergence. Negative correlation between energy of germination and pigments and positive correlation between seedling emergence and pigments in the parsnip and parsley varieties was observed. Determination the content of pigments and correlations observed the possibility to use spectrophotometry to evaluate the pigments present in the seeds as an indicator of seed quality.

Autofluorescence-spectral imaging as an innovative method for rapid, non-destructive and reliable assessing of soybean seed quality

“…Such mechanism may be related to an increase in chlorophyll a and b in aged seeds and higher amount of chlorophyll a in the resulting seedlings to improve the conversion of light to chemical energy—although this was not sufficient to improve the photochemical activity of the photosystem II as revealed by F V /F M values. In fact, chlorophyll plays an essential role in the physiological performance of mature seeds 36 , 37 , and plant responses to abiotic stress conditions 38 .…”

Integrating Optical Imaging Tools for Rapid and Non-invasive Characterization of Seed Quality: Tomato (Solanum lycopersicum L.) and Carrot (Daucus carota L.) as Study Cases

“…This result validates the findings of Jalink et al ( 1998 ) who showed that the magnitude of chlorophyll fluorescence in seeds was inversely related to the germination performance and/or to the capacity for generation of normal seedlings in cabbage ( Brassica oleracea ). Seeds with non-degraded chlorophyll are more susceptible to deterioration due to the production of harmful compounds derived from chlorophyll oxidation, such as singlet oxygen ( 1 O 2 ) (Grulichova et al, 2018 ), which is toxic to cells and involved in the programmed cell death (Triantaphylidès and Havaux, 2009 ).…”

Correlation of photosynthetic pigments content with indicators of seed quality in the seeds of carrot, celery, dill, parsley, and parsnip

“…The spectrophotometric measurement of chlorophylls and carotenoids in mature seeds has been successfully used in other studies, that was performed with various solvents such as acetone (Allorent et al, 2015), dimethylformamide (Zinsmeister et al, 2016), methanol (Smolikova et. al., 2018), or petroleum ether and tetrahydrofuran (Bulda et al, 2008;Smolikova et al, 2011;Grulichova et al, 2018).…”