Determination of Chloramphenicol in Bovine Milk by Liquid Chromatography/Tandem Mass Spectrometry

Abstract

A rapid confirmatory liquid chromatographic/tandem mass spectrometic method was developed for determination of chloramphenicol in bovine milk. Chloramphenicol was extracted directly from milk by solid-phase extraction on a C18 cartridge. The extract was further cleaned up on neutral aluminium oxide. Three transition products were monitored in negative ion mode after atmospheric pressure chemical ionization. The detection capability related to the transition product of lowest abundance was 0.03 μg/kg. The mean recovery was 90% at levels of 0.1–0.2 μg/kg. The relative repeatability standard deviations were 4.3, 3.8, and 2.8% at levels of 0.1, 0.2, and 1.0 μg/kg, respectively.

Determination of chloramphenicol in aquatic products by graphene-based SPE coupled with HPLC-MS/MS

“…Recently, several analytical methods have been developed for the analysis and determination of CAP in food. HPLC is commonly used procedure with different detection techniques such as UV diode array detection , electrochemical detection , and MS/MS . However, analyte pretreatment and cleanup procedures were always time consuming and organic solvent costing.…”

Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin

“…Chloramphenicol (CHL, 2,2-dichloro-N-((1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl) propan-2-yl)acetamide, a chemical structure shown in Figure 1) is a broad bacteriostatic antimicrobial; it exhibits antibacterial activity against both gram-positive and gram-negative bacteria, as well as other groups of microorganisms [1,2]. CHL has also been proven to be effective against epidemic meningococcal meningitis [3]; it exerts its antimicrobial effect by inhibiting bacterial protein synthesis through binding to the 50S ribosomal subunit to interfere with the requisite peptidyl transferase [4].…”

A competitive enzyme‐linked immunosorbent assay for determination of chloramphenicol

“…Current methods available for measuring concentrations of the drug in animal body fluids are expensive and not readily available in the developing countries. These methods include, gas-liquid chromatography (GLC) (Sorensen et al, 2003;Takino et al, 2003), streptavidin-biotin enzymelinked immunosorbent assay (ELISA) (van de Water & Haagsma, 1990), an ELISA (van de Water et al, 1987) and a solid-phase immunoenzyme (Kolosova et al, 1999). Despite the fact that these methods have been developed the appropriate conjugates and the antibodies are not readily available in the market.…”

“…Current methods available for measuring concentrations of the drug in animal body fluids are expensive and not readily available in the developing countries. These methods include, gas‐liquid chromatography (GLC) (Sorensen et al. , 2003; Takino et al.…”