Determination of Cell Volume Changes by an Inulin–Urea Assay in 96-Well Plates: A Comparison with Coulter Counter Analysis

Oehler, Rudolf; Hefel, Beate; Roth, Erich

doi:10.1006/abio.1996.0412

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Measurement Of Man-6-p and Atp In Embryos And1

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Cited by 9 publications

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“…Cellular concentrations of M6P were determined in PERK −/− MEFs after quantification from known cell numbers by FACE, and measurement of cellular volume by the [ 3 H]inulin-[ 14 C]urea method (Oehler et al , 1996). …”

Section: Methodsmentioning

confidence: 99%

Mannose-6-phosphate regulates destruction of lipid-linked oligosaccharides

Gao

Shang

Huynh

et al. 2011

MBoC

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Section: Methodsmentioning

confidence: 99%

Mannose-6-phosphate regulates destruction of lipid-linked oligosaccharides

Gao

Shang

Huynh

et al. 2011

MBoC

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“…Man-6-P amounts normalized to protein content in fibroblasts were adjusted to cell volumes to calculate intracellular Man-6-P concentrations. Cell volumes in Mpi ϩ/ϩ and Mpi Ϫ/Ϫ fibroblasts were determined using a method based on exclu- (25). The ATP content in embryos and fibroblasts was determined using a modified method according to Stocchi et al (26).…”

Section: Measurement Of Man-6-p and Atp In Embryos Andmentioning

confidence: 99%