Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study

Buch, Karl; Peters, Tanja; Nawroth, Thomas; Sänger, Markus; Schmidberger, Heinz; Langguth, Peter

doi:10.1186/1748-717x-7-1

Cited by 222 publications

(184 citation statements)

References 10 publications

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“…the MTT assay) in comparison to colony forming. It has previously been shown that tetrazolium--based assays to some degree overestimate the surviving fraction for absorbed doses over approximately 8−10 Gy (27). But since we limited this study to maximum 10 Gy and also used the WST--1 assay for all our evaluations, we believe that the comparison of the effects of gamma and alpha--particle irradiations are comparable.…”

Section: Radiation Sensitivity Measurementsmentioning

confidence: 94%

Cancer Cell Radiobiological Studies Using In-House-Developed α-Particle Irradiator

Nilsson

Bauden²,

Nilsson³

et al. 2015

Cancer Biotherapy and Radiopharmaceuticals

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An alpha--particle irradiator, enabling high--precision irradiation of cells for in--vitro studies, has been constructed. The irradiation source was a 241 Am source, on which well inserts containing cancer cells growing in monolayer were placed. The total radioactivity, uniformity, and alpha--particle spectrum were determined by use of HPGe--detector, Gafchromic™--dosimetry film, and PIPS®--detector measurements, respectively. Monte Carlo simulations were used for dosimetry. Three prostate cancer (LNCaP, DU145, PC3) and three pancreatic cancer (Capan--1, Panc--1, BxPC--3) cell lines were irradiated by alpha particles to the absorbed doses 0, 0.5, 1, and 2 Gy. For reference, cells were irradiated using 137 Cs to the absorbed doses 0, 1, 2, 4, 6, 8, and 10 Gy. Radiation sensitivity was estimated using a tetrazolium salt--based colorimetric assay with absorbance measurements at 450 nm. The relative biological effectiveness for alpha particles relative to gamma irradiation at 37% cell survival for the LNCaP, DU145, PC3, Capan--1, Panc--1, and BxPC--3 cells were 7.9 ± 1.7, 8.0 ± 0.8, 7.0 ± 1.1, 12.5 ± 1.6, 9.4 ± 0.9, and 6.2 ± 0.7, respectively. The results show the feasibility of constructing a desk--top alpha--particle irradiator, as well as indicate that both prostate and pancreatic cancers are good candidates for further studies of alpha--particle radioimmunotherapy.

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Section: Radiation Sensitivity Measurementsmentioning

confidence: 94%

Cancer Cell Radiobiological Studies Using In-House-Developed α-Particle Irradiator

Nilsson

Bauden²,

Nilsson³

et al. 2015

Cancer Biotherapy and Radiopharmaceuticals

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“…The total number of colonies containing >50 cells were counted using a binocular light microscope (Olympus Corporation, Tokyo, Japan). Following the counting of the colonies, the plating efficiency (PE) and survival fraction (SF) were calculated using the following equations (22,23).…”

Section: Methodsmentioning

confidence: 99%

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

et al. 2016

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“…Multiple-MTS assay was used to estimate the survival fraction (17). In multiple-MTS assay, the cell growth data (the number of viable cells) are collected daily to draw the cell growth curve.…”

Section: Survival Fraction-based Multiple-mts Assaymentioning

confidence: 99%

Gold Nanoparticle and Mean Inactivation Dose of Human Intestinal Colon Cancer HT-29 Cells

Arab‐Bafrani

Saberi

Birgani

et al. 2015

Jundishapur J Nat Pharm Prod

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Background: Mean inactivation dose is a useful radiobiological parameter for the comparison of human cell survival curves. Objectives: Given the importance and accuracy of these parameters, in the present study, the radio sensitivity enhancement of colon cancer (HT-29) cells in the presence of gold nanoparticles (GNPs) were studied using the mean inactivation dose (MID). Materials and Methods: Naked-GNPs with 50 nm diameters were incubated with HT-29 cells. The cytotoxicity and uptake of these particles on HT-29 cells were assessed. After determining the optimum GNPs concentration, the cells were incubated with gold nanoparticle for 24 hours. The change in the MID value as well as the radio sensitization enhancement under irradiation with 9 MV X-ray beams in the presence of GNPs were evaluated by multiple (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) MTS assay. Results: Cell survival in the presence of GNPs was more than 90% and the maximum uptake of GNPs was observed at 60 µM of gold nanoparticles. In contrast, in the presence of GNPs combined with radiation, cell survival and MID value significantly decreased, so that the radio sensitization enhancement was 1.4. Conclusions: Due to the significant reduction in the mean inactivation dose of colon cancer cells in the presence of gold nanoparticles, it seems that GNPs are suitable options to achieve a new approach in order to improve radiotherapy efficiency without increasing the prescribed radiation dose.

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Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study

Cited by 222 publications

References 10 publications

Cancer Cell Radiobiological Studies Using In-House-Developed α-Particle Irradiator

Cancer Cell Radiobiological Studies Using In-House-Developed α-Particle Irradiator

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

Gold Nanoparticle and Mean Inactivation Dose of Human Intestinal Colon Cancer HT-29 Cells

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