Determination of cell electroporation in small-volume samples by using a mini potassium-selective electrode

Saulis, Gintautas; Pranevičiūtė, Rita

doi:10.1016/j.ab.2005.06.034

Cited by 7 publications

(11 citation statements)

References 8 publications

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“…Experimental data show that 'threshold' electroporation of cells and liposomes can involve pores just large enough to let water and small ions through, but still impermeable to slightly larger molecules, such as mannitol (molecular weight M w = 182.17 Da), sucrose (M w = 342.3 Da), or propidium iodide [53,[57][58][59]. The radii of these molecules are in the range of 0.4-0.6 nm.…”

Section: Cell Electroporationmentioning

confidence: 99%

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Electroporation of Cell Membranes: The Fundamental Effects of Pulsed Electric Fields in Food Processing

Saulis

2010

Food Eng. Rev.

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199

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Using of pulsed electric fields (PEF) for killing of microorganisms in liquid foods is a promising new nonthermal food processing and preservation technology. However, to implement and optimize this technology, a good understanding of the actual mechanisms that govern microbial inactivation by this technique is required. Here, fundamentals of cell electroporation, which is considered as underlying phenomenon of food processing technology, are discussed. The whole process of the cell electroporation (food processing) by PEF is divided into the following four main stages: (1) building the transmembrane potential up by the applied external electric field, (2) creation of small metastable hydrophilic pores, when the transmembrane potential has been built up; (3) evolution of the pore population-the change in the number and/or sizes of poresduring an electric treatment; and (4) post-treatment stage consisting of the processes that take place after the electric treatment (leakage of intracellular compounds, pore shrinkage and disappearance, etc.). The current knowledge of the processes taking place during each of the above stages as well as the factors influencing them is discussed. Theoretical considerations are illustrated with the experimental data available.

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Section: Cell Electroporationmentioning

confidence: 99%

“…, and Na ? ions are in the range of 0.16-0.33 nm [61], which means that the smallest radius of created pores can be in the range of 0.3 nm [57,60,62]. Because of this, using the test molecules that are larger than the small ions of K ?…”

Section: Cell Electroporationmentioning

confidence: 99%

Electroporation of Cell Membranes: The Fundamental Effects of Pulsed Electric Fields in Food Processing

Saulis

2010

Food Eng. Rev.

Self Cite

199

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“…So, the fraction of electroporated cells can be determined from the extracellular concentration of K + ions [8,9]. In this study, the dependences of the fraction of electroporated cells on the pulse intensity were obtained for the cells exposed to a single square-wave electric pulse with the duration of 0.02-2 ms.…”

Section: Resultsmentioning

confidence: 99%

“…Blood was collected on sodium citrate and the erythrocytes were isolated by centrifugation. The erythrocytes were then washed three times with isotonic sodium chloride solution and suspended in the electroporation medium at a volume concentration of 2-2.5% [8]. CHO and rat glioma C6 cells were grown in monolayer cultures in 75 cm 2 (200 ml) flasks at 37…”

Section: Methodsmentioning

confidence: 99%

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Comparison of Electroporation Threshold for Different Cell Lines in vitro

Saulis

Saulė

2009

Acta Phys. Pol. A

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The electroporation threshold was compared at various electric pulse durations for three cell lines: two non-tumor cell lines (human erythrocytes and Chinese hamster ovary cells) and one tumor cell line (rat glioma C6 cells). First, the dependences of the fraction of electroporated cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations of 0.02-2 ms. Then, the average cell radii were measured for each cell line and the transmembrane potential induced by the external electric field was calculated. The obtained values of the transmembrane potential were in the range of 480-930 mV and decreased with increasing pulse duration. The obtained dependences of the transmembrane potential required to electroporate 50% of cells on the pulse duration were close to each other for all cell lines studied.

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The Electroporation as a Tool for Studying the Role of Plasma Membrane in the Mechanism of Cytotoxicity of Bisphosphonates and Menadione

et al. 2016

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In this study, the role of the cell plasma membrane as a barrier in the mechanism of the cytotoxicity of nitrogen-containing bisphosphonates and menadione was studied, and the possibility of increasing the efficiency of bisphosphonates and menadione (vitamin K) as chemotherapeutic agents by permeabilizing the cell plasma membrane has been investigated in vitro. The plasma membrane barrier was reduced by electropermeabilization with the pulse of strong electric field. Two membrane-impermeant bisphosphonates with different hydrophilicities were chosen as study objects: ibandronate and pamidronate. For the comparison, an amphiphilic vitamin K, which is able to cross the cell membrane, was studied as well. The impact of nitrogen-containing bisphosphonates and vitamin K on MH-22A cells viability was evaluated for the case of long (9 days) and short (20 min) exposure. When cells were cultured in the medium with vitamin K for 9-10 days, it exhibited toxicity of 50 % over the control at 6.2 µM for mouse hepatoma MH-22A cells. Ibandronate and pamidronate were capable of reducing drastically the cell viability only in the case of long 9-days incubation and at high concentrations (~20 µM for pamidronate and over 100 µM for ibandronate). Single, square-wave electric pulse with the duration of 100 µs and the field strength of 2 kV/cm was used to electroporate mouse hepatoma MH-22A cells in vitro. The results obtained here showed that the combination of the exposure of cells to membrane-impermeable bisphosphonates pamidronate and ibandronate with electropermeabilization of the cell plasma membrane did not increase their cytotoxicity. In the case of membrane-permeable vitamin K, cell electropermeabilization did increase vitamin K killing efficiency. However, this increase was not substantial, within the range of 20-30 % depending on the duration of the exposure. Electropermeabilization improved cytotoxic effect of vitamin K but not of pamidronate and ibandronate.

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Determination of cell electroporation in small-volume samples by using a mini potassium-selective electrode

Cited by 7 publications

References 8 publications

Electroporation of Cell Membranes: The Fundamental Effects of Pulsed Electric Fields in Food Processing

Electroporation of Cell Membranes: The Fundamental Effects of Pulsed Electric Fields in Food Processing

Comparison of Electroporation Threshold for Different Cell Lines in vitro

The Electroporation as a Tool for Studying the Role of Plasma Membrane in the Mechanism of Cytotoxicity of Bisphosphonates and Menadione

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