Determination of CD4+ and CD8+ Lymphocytes with the Cytosphere Assay: A Comparative Study with Flow Cytometry and the Immunoalkaline Phosphatase Method

Gernow, Anne; Lisse, Ida Maria; Böttiger, Blenda; Christensen, L B; Brattegaard, K.

doi:10.1006/clin.1995.1107

Cited by 18 publications

(16 citation statements)

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“…Other studies compared the Cyto-Spheres assay with dual platform flow cytometry (DPFC) and detected intermediate to high correlations (r ¼ 0.74 to r ¼ 0.93) (8,(17)(18)(19)(20), whereas only one study found a substantially lower correlation (r ¼ 0.45) (6).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Karcher¹,

Böhning

Downing

et al. 2006

Cytometry Part B Clinical

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Section: Discussionmentioning

confidence: 99%

“…In addition, the quality of the results depends on individual skills and experience of the laboratory technician. Results may also be less accurate due to the low number of total cells counted (250) per analysis (6,8,(17)(18)(19)(20).…”

Section: Advantages and Disadvantages Of The Manual Cyto-spheres Methmentioning

confidence: 99%

Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Karcher¹,

Böhning

Downing

et al. 2006

Cytometry Part B Clinical

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“…Although this bead-based flow cytometric method eliminates the need for multiple technologies, it is still limited by the high cost of the currently available FCMs and fluorescent beads. Although alternative nonflow cytometric technologies are simple and less expensive, they have not been implemented widely due to their complexity, low-volume CD4 counting application (1 to 10 samples/day), and poor quality control (4,5,12,22). The recent introduction of the inexpensive single-parameter CyFlow green FCM (Partec GmbH, Münster, Germany), which uses a single phycoerythrin (PE) conjugated-monoclonal antibody to CD4, has increased access to CD4 ϩ count determinations for patients in areas that are not equipped with an FCM, particularly in district hospitals in the rural areas of Thailand.…”

mentioning

confidence: 99%

Evaluation of a New Single-Parameter Volumetric Flow Cytometer (CyFlow ^green ) for Enumeration of Absolute CD4 ⁺ T Lymphocytes in Human Immunodeficiency Virus Type 1-Infected Thai Patients

Pattanapanyasat

Lerdwana

Noulsri

et al. 2005

Clin Vaccine Immunol

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“…We have previously tested another alternative method for T cell determination in both the Ivory Coast and Denmark, namely the Cytosphere Assay (CA) from Coulter [7]. Among HIVseropositive individuals, sensitivity and specificity was 93% and 73% for a CA CD4 T lymphocyte count of < 200 cells/ml to detect an FC CD4 T lymphocyte count of < 200 cells/ml, and a sensitivity and specificity of 54% and 88% for a CA CD4 percentage < 14% to detect an FC CD4 percentage < 14% (data available on request).…”

Section: Comparison Of T Cell Subsets Methods In Africa Andmentioning

confidence: 99%

Evaluation of T Cell Subsets by an Immunocytochemical Method Compared to Flow Cytometry in Four Countries

Lisse

Böttiger²,

Christensen

et al. 1997

Scand J Immunol

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The authors tested an alternative method for CD4 and CD8 T lymphocytes enumeration, the immunoalkaline phosphatase method (IA), in three African countries and in Denmark. The IA determinations from 136 HIV antibody positive and 105 HIV antibody negative individuals were compared to the corresponding results obtained by flow cytometry (FC) performed in the respective countries. The authors found good correspondence between the two methods for measurements of CD4 and CD8 T lymphocytes independent of serological status and geographical site. However, the CD4 and CD8 T lymphocyte values obtained by the two methods are not interchangeable as IA compared to FC consistently gives higher percentage of CD4 T lymphocytes, and lower percentages of CD8 T lymphocytes. Mean differences between the two methods did not differ between the three African countries indicating that the IA method provides systematic results. Replicate measurements suggested good correspondence between results obtained by IA. By using an IA level of < 300 CD4 T lymphocytes/ml, the sensitivity was 81% and specificity 96% for detecting an FC level of < 200 CD4 T lymphocytes/ml. Using an IA level of < 20% CD4 T lymphocytes, the sensitivity was 89% and specificity 95% for detecting an FC level of <14% CD4 T lymphocytes, The FC and IA methods had the same internal correspondence between low absolute CD4 T cell counts and low CD4 percentages; the sensitivity and specificity for detecting a low absolute CD4 T cell count with a low CD4 percentage was 92% and 68% for FC and 91% and 73% for IA, respectively. The IA method is 10-fold cheaper than FC, is independent of advanced laboratory facilities, and does not need immediate processing of samples as blood smears can be stored for long periods. The IA method is therefore suitable for use in areas with limited resources and laboratory facilities where there is a need for immunological surveillance in hospital or community studies.

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Determination of CD4+ and CD8+ Lymphocytes with the Cytosphere Assay: A Comparative Study with Flow Cytometry and the Immunoalkaline Phosphatase Method

Cited by 18 publications

References 0 publications

Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Evaluation of a New Single-Parameter Volumetric Flow Cytometer (CyFlow ^green ) for Enumeration of Absolute CD4 ⁺ T Lymphocytes in Human Immunodeficiency Virus Type 1-Infected Thai Patients

Evaluation of T Cell Subsets by an Immunocytochemical Method Compared to Flow Cytometry in Four Countries

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Determination of CD4+ and CD8+ Lymphocytes with the Cytosphere Assay: A Comparative Study with Flow Cytometry and the Immunoalkaline Phosphatase Method

Cited by 18 publications

References 0 publications

Comparison of two alternative methods for CD4+ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Comparison of two alternative methods for CD4+ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Evaluation of a New Single-Parameter Volumetric Flow Cytometer (CyFlow green ) for Enumeration of Absolute CD4 + T Lymphocytes in Human Immunodeficiency Virus Type 1-Infected Thai Patients

Evaluation of T Cell Subsets by an Immunocytochemical Method Compared to Flow Cytometry in Four Countries

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Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Comparison of two alternative methods for CD4⁺ T‐cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda

Evaluation of a New Single-Parameter Volumetric Flow Cytometer (CyFlow ^green ) for Enumeration of Absolute CD4 ⁺ T Lymphocytes in Human Immunodeficiency Virus Type 1-Infected Thai Patients