Determination of catecholamines in urine by ion-exchange liquid chromatography with electrochemical detection

Eriksson, Britt‐Marie; Gustafsson, Stina; Persson, Bengt‐Arne

doi:10.1016/s0378-4347(00)84784-2

Cited by 35 publications

(5 citation statements)

References 16 publications

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“…Sensitivity 0.3 lUI/ml; inter-and intra-assay CV lower than 4.8% and 2.6%, respectively). Catecholamines were measured by using high-performance liquid chromatography for electrochemical detection (Eriksson et al 1983). …”

Section: Methodsmentioning

confidence: 99%

Decreased serum leptin levels during prolonged high altitude exposure

et al. 2004

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Circulating leptin concentrations are shown to be influenced not only by hormones, but also by body weight and energy balance. High altitude (HA) exposure induces a daily negative energy balance and stress hormone activation. The aim of our study was to evaluate leptin concentration during both acute and prolonged HA exposure and its correlations with some metabolic and hormonal parameters. Twelve males were studied during a stay at HA (15-20 days at 5,050 m). Blood samples for serum leptin, plasma insulin and 24-h urinary epinephrine (E) and norepinephrine (NE) were collected at sea level (SL), at the arrival at HA (A) and after 12-16 days (C) of stay. Symptoms of Acute Mountain Sickness (AMS) were evaluated using the Lake Louise score and the results showed there was no relationship with leptin concentrations. During the stay, both body mass index and leptin levels significantly decreased in both groups [leptin from 1.88 (1.12) to 1.21 (1.04) ng/ml, P<0.008, in A; and to 1.06 (0.74) ng/ml, P<0.003, in C]. Acute HA exposure induced a clear-cut significant increase of NE ( P<0.001 in A, P<0.003 in C) while E and insulin levels were unchanged in both phases. Moreover, a significant correlation between leptin and NE absolute values, and leptin and insulin variations was found ( r 0.359, P<0.034 and r=0.560, P<0.007, respectively). Exposure to HA induces a decrease in fasting serum leptin concentrations in men. These changes are not linked to symptoms of AMS but to hormonal and energy balance variations, suggesting that leptin is involved in the endocrine and metabolic adaptations occurring during HA exposure.

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Section: Methodsmentioning

confidence: 99%

Decreased serum leptin levels during prolonged high altitude exposure

et al. 2004

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“…No: 68548, Espoo, Finland). A total of 24 h U NA/A was analysed with an electrochemical detection method according to Eriksson et al [16], and we used the quotient of norepinephrine and epinephrine, as it reflects catecholaminergic metabolism.…”

Section: Analyses Of Biochemical Markersmentioning

confidence: 99%

Suicide attempters: biological stressmarkers and adverse life events

Sunnqvist

Westrin

Träskman‐Bendz

2008

Eur Arch Psychiatry Clin Neurosc

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j Abstract Risk factors for suicidal behaviour include adverse life events as well as biochemical parameters acting, e.g. within the hypothalamic-pituitary-adrenal axis and/or monoaminergic systems. The aim of the present investigation was to study stressful life events and biological stress markers among former psychiatric inpatients, who were followed up 12 years after an index suicide attempt. At the time of the index suicide attempt, and before treatment, cerebrospinal fluid (CSF) samples were taken, and 24 h (h) urine (U) was collected. 3-Methoxy-4-hydroxyphenylglycole (MHPG) in CSF and 24 h urinary samples of cortisol and noradrenaline/adrenaline (NA/A) were analysed. Data concerning stressful life events were collected retrospectively from all participants in the study through semi-structured interviews at follow-up. We found that patients who reported sexual abuse during childhood and adolescence had significantly higher levels of CSF-MHPG and U-NA/A, than those who had not. Low 24 h U-cortisol was associated with feelings of neglect during childhood and adolescence. In conclusion, this study has shown significant and discrepant biological stress-system findings in relation to some adverse life events. j Key words suicide attempt AE catecholaminergic markers AE U-cortisol AE adverse life events

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“…Increased activity in sympathetic nerves is reflected by increases in NE (Udenfriend & Dairman, 1971) and general activation of the sympathetic nervous system initiates changes in EPI (Nesse, Cameron, Curtis, McCann, & Huber-Smith, 1984). The contribution of the NE nerve activity leads to baseline levels comparable to those of adrenally released EPI mirrors the relative levels humans (Eriksson, Gustafsson, & Persson, 1983). The consistent DA over these time points, along with the consistent creatinine levels, suggests that the increases in NE and EPI are not due to an increase in kidney function or production of urine (McDonald, Goldberg, McNay, & Tuttle, 1964).…”

Section: Discussionmentioning

confidence: 76%

Measurement of urinary catecholamines in small samples for mice

Smith

Schwartz

Lucot

2013

Journal of Pharmacological and Toxicological Methods

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Introduction Analysis of catecholamines in small samples of urine is difficult and sensitive to stress. Current techniques require pooling of samples or expensive separation by double mass spectrometry. A method for extraction of unconjugated catecholamines in 20μL urine samples has been developed using alumina extraction prior to separation by high performance liquid chromatography (HPLC) and electrochemical detection (ECD). Methods Three murine experiments tested the application of the procedure. In the first, collection occurred in the morning and evening prior to handling, and in the morning after three days of handling. In the second, passively obtained urine was compared to stressfully obtained urine in the same mice. Finally, basal collections were compared to urinary catecholamine levels 15 and 30 minutes into novel cage stress. Urine was extracted alongside 2,3-dihydroxybenzoic acid (DHBA) internal standard via alumina and brought to pH 8.5 with tris buffer. The mixture underwent two wash steps for depuration and eluted with perchloric acid for analysis on HPLC with ECD. Results This novel extraction method using low amounts of urine yielded 48% recovery in the samples and 60% recovery in the standard extraction on average. With a signal to noise ratio of 3:1, the limit of detection (LOD) of a standard is 1.2pg/mL, which allows for the detection of 3.6pg/mL in urine or 72fg in a 20μL sample. Thus resting catecholamine levels are 216 times higher than the LOD. Unconjugated norepinephrine and epinephrine levels were significantly increased 15 minutes after novel cage stress and epinephrine remained elevated after 30 minutes, but did not show significant differences when comparing collection time, handling exposure, or specific collection technique. Discussion The technique is an effective measure for sympathetic activity in micro samples, with a limit of detection in the attomole range for 20μL samples.

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Determination of catecholamines in urine by ion-exchange liquid chromatography with electrochemical detection

Cited by 35 publications

References 16 publications

Decreased serum leptin levels during prolonged high altitude exposure

Decreased serum leptin levels during prolonged high altitude exposure

Suicide attempters: biological stressmarkers and adverse life events

Measurement of urinary catecholamines in small samples for mice

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