Determination of catecholamines and their metabolites in human plasma using liquid chromatography with coulometric multi-electrode cell-design detection

Unceta, Nora; Rodríguez, Esther; Balugera, Zuriñe Gómez de; Sampedro, Carmen; Goicolea, M. Aránzazu; Barrondo, Sergio; Sallés, Joan; Barrio, Ramón J.

doi:10.1016/s0003-2670(01)01215-6

Cited by 36 publications

(24 citation statements)

References 38 publications

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“…When compared to the other methods which use SPE procedures [14,17], this method has the advantage of giving better extraction yields [14] and being more rapid and feasible [17].…”

Section: Discussionmentioning

confidence: 99%

“…This method had distinct advantages over other extraction procedures, such as those using activated alumina [2,19 -20], or liquid-liquid extraction [21,22], due to its high extraction yield and precision. In the last few years, other authors have proposed SPE procedures with reversed-phase sorbents and diphenylborate complexation [14] or with cationic exchange sorbents [17]; in the latter case, however, a 35-times dilution is carried out to elute the sample, thus a subsequent derivatisation is needed in order to obtain a sufficient sensitivity for the analysis of CAs in human plasma.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion‐exchange procedure

Raggi¹,

Sabbioni

Nicoletta

et al. 2003

J of Separation Science

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Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion-exchange procedure Simultaneous determination of the three main catecholamines (Epinephrine, Norepinephrine, and Dopamine) has been implemented by means of liquid chromatography with amperometric detector working in oxidation at +800 mV. The chromatographic analysis was carried out on a reversed phase column (C8 Rainin 15064.6 mm ID, 5 lm) with a mixture of methanol and an aqueous solution of 5 mM of citric acid, 20 mg L -1 octanesulfonic acid, and 20 mg L -1 EDTA (pH 2.9) as the mobile phase. An original solid phase extraction pretreatment of plasma samples was developed using cationic ionic exchange cartridges with carboxypropylic adsorbent. The steps of the SPE procedure were carefully checked and optimal elution was carried out with perchloric acid. High extraction yield values were obtained for all catecholamines with good reproducibility. Linearity was observed in the 0.06 -3.00 ng mL -1 concentration range for all analytes. LOD and LOQ were respectively 0.03 ng mL -1 and 0.06 ng mL -1 . The method was applied successfully to the analysis of some real plasma samples from young volunteers under experimental psychological stress.

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“…When compared to the other methods which use SPE procedures [14,17], this method has the advantage of giving better extraction yields [14] and being more rapid and feasible [17].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion‐exchange procedure

Raggi¹,

Sabbioni

Nicoletta

et al. 2003

J of Separation Science

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“…The linear range was 0.5-5000 pg with r > 0.999 (Takeda et al, 1990). A solid-phase extraction method combined with liquid chromatography with coulometric multi-electrode detection improved the detection limit to 0.01-0.05 ng/L, and nine catecholamine-related compounds were separated within 40 min (Unceta et al, 2001).…”

Section: Original Researchmentioning

confidence: 96%

Semi‐micro liquid chromatography of aromatic amino acid metabolites using isocratic elution and Column switching

Hanai¹,

Kaneko

Homma

2002

Biomedical Chromatography

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The concentrations of catecholamine-related compounds in body fluids reflect sympathetic nerve functions. Measuring the enzyme activity of these metabolic pathways will improve diagnosis since a variety of symptoms are reported. An isocratic elution system with two column switching valves was developed using three types of semi-micro columns for fast chromatographic analysis of catecholamine related compounds. Columns are a pentyl-bonded phase, 50 x 2.1 mm i.d., a phenylhexyl-bonded phase, 100 x 2.1 mm i.d. and an octadecyl-bonded phase, 100 x 2.1 mm i.d. The separation of 20 standard compounds was achieved within 25 min using reversed-phase ion-pair liquid chromatography with an electrochemical detector. This new system was applied for analysis of catecholamine-related compounds in pig brain, since pigs are a widely used animal model for transgenic manipulation of neural genes, and MHPG (or VMA), DOPAC, DOPA, NE, EP, DA, 5HTP and 5HIAA were quantified.

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“…This method is based on the creation of diasteroisomers from DCIT and DDCIT (−)-(R) and (+)-(S) enantiomers and their subsequent chromatographic separation using normal-phase chromatography. In addition, owing to the fact that SPE involves multiple steps and it could be labour-intensive and time-consuming (Unceta et al, 2001), a new and simple liquid-liquid extraction procedure has been improved for the isolation of rac-DCIT and rac-DDCIT from plasma and brain tissue samples. HPLC-grade solvents tetrahydrofuran, chloroform, isooctane, hexane and isopropanol were purchased from Scharlab (Barcelona, Spain).…”

Section: Introductionmentioning

confidence: 99%

Stereoselective determination of demethyl‐ and didemethyl‐citalopram in rat plasma and brain tissue by liquid chromatography with fluorescence detection using precolumn derivatization

Millán

Goicolea

Sánchez

et al. 2007

Biomedical Chromatography

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A rapid and sensitive HPLC enantioselective method with fluorescence detection was developed to determine (-)-(R) and (+)-(S) enantiomers of the metabolites of citalopram, demethyl- and didemethyl-citalopram in plasma and brain tissue. This assay involves pre-column chiral derivatization with (-)-(R)-1-(1-naphthyl)ethyl isocyanate followed by separation on a normal-phase silica column. The developed liquid-liquid extraction procedure permits quantitative determination of analytes with recoveries ranged between 81 and 88% with intra- and inter-day relative standard deviations less than 10.5%. Linearity was obtained over the concentration range 5-1000 ng/mL and 100-10,000 ng/g for spiked drug-free plasma and brain tissue, respectively, with detection limits lower than 2.1 ng/mL and 42.8 ng/g.

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Determination of catecholamines and their metabolites in human plasma using liquid chromatography with coulometric multi-electrode cell-design detection

Cited by 36 publications

References 38 publications

Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion‐exchange procedure

Analysis of plasma catecholamines by liquid chromatography with amperometric detection using a novel SPE ion‐exchange procedure

Semi‐micro liquid chromatography of aromatic amino acid metabolites using isocratic elution and Column switching

Stereoselective determination of demethyl‐ and didemethyl‐citalopram in rat plasma and brain tissue by liquid chromatography with fluorescence detection using precolumn derivatization

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