Determination of (+)-Catechin and Antioxidant Activity in Faloak (Sterculia quadrifida R. Br) Stem Bark Infusion

Abstract

Oxidative stress is a condition that can damage human cells and tissues and has been linked to a number of illnesses, including cancer, cardiovascular disease, autoimmune disorders, and neurological diseases. Oxidative stress conditions can be brought on by pollution, radiation exposure, and an unhealthy lifestyle. Antioxidants are substances that can be used to both prevent and treat oxidative stress. This study aimed to identify and quantify (+)-catechin levels and antioxidant activity of the stem bark of Sterculia quadrifida R. Br extracted by the infusion method, a method similar to traditional medicine processing generally in the community. Determination of (+)-catechin and antioxidant activity of S. quadrifida were evaluated by HPLC and DPPH assay, respectively. Quantification of (+) catechin content by HPLC system with wavelength 280 nm and antioxidant activity by spectrophotometry method with wavelength 517 nm. The results show that the mean value of (+)-catechin level was 7.786% and the IC50 value of the antioxidant activity was 51.5 ug/mL having a moderate antioxidant activity category. S. quadrifida stem bark infusion can be utilized as a medication candidate for the prevention or treatment of a variety of disorders caused by oxidative stress.

Anti-inflammatory effect of Faloak (Sterculia quadrifida R. Br) stem bark on TNF-α, IL-1β, and IL-6 in DENV-3-infected Wistar rats

“…The stem bark of S. quadrifida is also recognized for its presence of catechin derivative chemicals, including epicatechin ( Dean et al , 2019 ) and (+)-catechin ( Riwu et al , 2023 ). Catechin is a compound that can regulate NF-κB, a transcription factor that regulates the production of different inflammatory genes like pro-inflammatory cytokines (such as TNF-α and IL-6).…”

“…The utilization of water as a solvent and heating it to a high temperature of up to 90°C during the extraction process can lead to the degradation of molecules that impact the amounts of certain chemicals and antioxidant activity that may affect the anti-inflammatory effects produced. However, the extraction using the infusion process is considered to be safer, more convenient, and more applicable to the community ( Hidalgo and Almajano, 2017 ; Réblová et al , 2017; Riwu et al , 2023 ; Xu et al , 2017 ).…”

“…The stem bark of S. quadrifida has been found to contain flavonoids, alkaloids, terpenoids, phenolic compounds, and saponins ( Siswadi and Saragih, 2019 ). In addition, specific compounds such as (+)-catechin ( Riwu et al , 2023 ), epicatechin ( Dean et al , 2019 ), scopoletin ( Munawaroh et al , 2020 ), and β-sitosterol ( Lulan, 2020 ) have been identified. Prior research has established that the stem bark exhibits potent antioxidant activity ( Amin et al , 2015 ; Dillak et al , 2019 ), and possesses immunomodulatory properties through the regulation of macrophage phagocytic activity, as well as the modulation of nuclear factor-kappaB and TNF-α (specifically in combination with Phyllanthus urinaria ) ( Winanta et al , 2019 ; Munawaroh et al , 2020 ; Rollando et al , 2020 ).…”

A Recent Update on the Potential Use of Catechins in Cosmeceuticals

“…Antioxidants scavenge oxygen free radicals (singlet and triplet), ROS, peroxide decomposers, and enzyme inhibitors to protect vital molecules from harm [22]. The use of antioxidants derived from natural ingredients is increasing.…”