Determination of cat sperm concentration using the CASA system

Schnitzer, Josiana de Fatima; Hidalgo, Myrian Megumy Tsunokawa; Trautwein, Luiz Guilherme Corsi; Almeida, Ana Beatriz Marques de; Souza, Anne Kemmer; Martins, Maria Isabel Mello

doi:10.34117/bjdv5n11-013

Cited by 1 publication

(1 citation statement)

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“…In addition, Schnitzer et al. (2019) reported that the CASA system is not a reliable method for sperm analysis when the concentration reading is below 401–800 cells per field. This finding is particularly relevant considering that the mean sperm concentration in our samples was 20 × 10 6 spermatozoa/mL.…”

Section: Discussionmentioning

confidence: 99%

Insights into alternative cryoprotectants to freeze sperm of domestic cats

Martins,

Silva,

Hidalgo

et al. 2024

Reprod Domestic Animals

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Globalization and habitat destruction pose a significant threat to wildlife felids. Even though conservation banks for genetic materials have been created, the sperm cryopreservation with minimal cell damage is still a great challenge. Thus, this study aimed to compare the effects of two commercial extenders with different concentrations of alternative cryoprotectants on thawed sperm quality of domestic cats. Five adult cats were anaesthetized (using a combination of 40 μg/kg medetomidine associated to 5 mg/kg ketamine), and the semen was collected by electroejaculation (electrical stimulation of 2–3 V). Semen samples were evaluated for sperm characteristics (kinetics, morphology, membrane integrity and morphometry). Subsequently, they were sorted into two aliquots and centrifuged. The aliquots were added to a commercial extender containing 3% glycerol and 2% methylformamide (extender I) or 2% glycerol and 3% methylformamide (extender II), frozen, thawed (37°C/30 s) and reevaluated. Comparatively, the sperm kinetics and membrane integrity of fresh semen were higher (p < .002) than frozen samples in extender I and II. Total and progressive motility were lowest in the thawed samples. However, the subjective analysis indicated high sperm motility, since the kinetics evaluation was impaired by the low cell number in the thawed samples. There were no differences in sperm morphology between the groups. In the sperm morphometric analysis, a significant difference (p = .04) was identified in the length of the intermediate piece in extender II samples compared with fresh and extender I. Thus, it can be concluded that although the concentrations tested did not maintain the kinetic parameters and membrane integrity of spermatozoa after thawing, the extender with a lower concentration of glycerol was less toxic for maintaining the midpiece length.

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Section: Discussionmentioning

confidence: 99%