Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography–electrospray ionization ion trap tandem mass spectrometry

Vernez, Laurence; Hopfgartner, Gérard; Wenk, Markus R.; Krähenbühl, Stephan

doi:10.1016/s0021-9673(02)01838-1

Cited by 80 publications

(65 citation statements)

References 28 publications

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“…Signals were preliminarily filtered by P < 0.05 and up-/downregulation factor (lower than −30% or higher than +30%) and manually processed to eliminate spurious peaks. The accurate mass list was searched against the METLIN web-based algorithm (50,51) (52), and, whenever possible, accurate MS/MS data, following the indications on MS/MS fragmentation patterns available in the literature (53)(54)(55)(56). The lipid species annotated are reported in SI Appendix, Table S1 (positive ion mode) and SI Appendix, Table S2 (negative ion mode).…”

Section: Methodsmentioning

confidence: 99%

Endogenous N -acyl taurines regulate skin wound healing

Sasso

Pontis

Armirotti

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The intracellular serine amidase, fatty acid amide hydrolase (FAAH), degrades a heterogeneous family of lipid-derived bioactive molecules that include amides of long-chain fatty acids with taurine [N-acyl-taurines (NATs)]. The physiological functions of the NATs are unknown. Here we show that genetic or pharmacological disruption of FAAH activity accelerates skin wound healing in mice and stimulates motogenesis of human keratinocytes and differentiation of human fibroblasts in primary cultures. Using untargeted and targeted lipidomics strategies, we identify two longchain saturated NATs-N-tetracosanoyl-taurine [NAT(24:0)] and N-eicosanoyl-taurine [NAT(20:0)]-as primary substrates for FAAH in mouse skin, and show that the levels of these substances sharply decrease at the margins of a freshly inflicted wound to increase again as healing begins. Additionally, we demonstrate that local administration of synthetic NATs accelerates wound closure in mice and stimulates repair-associated responses in primary cultures of human keratinocytes and fibroblasts, through a mechanism that involves tyrosine phosphorylation of the epidermal growth factor receptor and an increase in intracellular calcium levels, under the permissive control of transient receptor potential vanilloid-1 receptors. The results point to FAAH-regulated NAT signaling as an unprecedented lipid-based mechanism of woundhealing control in mammalian skin, which might be targeted for chronic wound therapy.FAAH | N-acyl taurines | FAEs | fibroblasts | keratinocytes

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Section: Methodsmentioning

confidence: 99%

Endogenous N -acyl taurines regulate skin wound healing

Sasso

Pontis

Armirotti

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Vernez et al [60] employed cation-exchange SPE of acidified urine as their sample cleanup. Subsequent ion-pairing RP-HPLC (Phenomenex LUNA 15062.0 mm, 5 mm) with 10 mM heptafluorobutric acid and 10 mM ammonium acetate aqueous mobile phase gradient-eluted from 20% to 90% methanol with MS n detection (positive-ion mode, 4.0 kV, N 2 sheath gas, 3007C capillary) afforded quantitation of free carnitine and acylcarnitines.…”

Section: Carnitinesmentioning

confidence: 99%

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

Smyth¹,

Brooks

2004

Electrophoresis

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A critical review of applications for the period 2000-2003, taken from the Web of Knowledge database, of the techniques high performance liquid chromatography-electrospray ionisation-mass spectrometry (HPLC-ESI-MS) and capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) to the detection and determination of small molecules of significance in clinical and forensic science is presented. The molecules of mass less than 500 Da are chosen according to selected structural classes in which they give ESI signals primarily as [M+H](+) ions although other ions such as [M-H](-), [M+Na](+) and [M+NH(4)](+) are also reported. The structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, carbohydrates, benzimidazoles, other heterocycles, sulphonylureas, anthracyclines, sulphonamides, penicillins, cephalosporins, tetracyclines, nitrocatechols, steroids, flavonoids, oxazaphosphorines, cannabinols, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole and time-of-flight mass spectrometers. The review then gives a critical evaluation of these recent HPLC-ESI-MS and CE-ESI-MS analytical methods for the detection and determination of small molecules of clinical and forensic significance. Analytical information on, for example, sample concentration techniques, HPLC and CE separation conditions, recoveries from biological media and limits of detection (LODs) are provided.

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“…Liquid chromatographyelectrospray mass spectrometry (LC-ESI-MS) has emerged as a sensitive analytical technique for the determination of various compounds (Grace et al, 2003;Bogialli et al, 2003;Vernez et al, 2003;Zhang et al, 2005;Fuh et al, 2004). The aim of this investigation is to develop a LC-ESI-MS method incorporated with microdialysis technique to determine sinomenine and study the pharmacokinetics of free-form sinomenine in rat skin.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

Zheng

Shi

Jin-hong

2006

Biomedical Chromatography

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Sinomenine (7,8-didehydro-4-hydroxy-3,7-dimethoxy-17-methylmorphinan-6-one) is a pure alkaloid extracted from the Chinese medical plant. In this report a liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) method with in vivo microdialysis for the pharmacokinetic study of free-form sinomenine in rat skin has been developed. A microdialysis probe was surgically implanted into the subcutaneous tissue of the rats and an isotonic phosphate buffer (PBS) was used as the perfusion medium. Samples were collected and then analyzed off-line by LC-ESI-MS. The chromatographic separation was achieved within 4.2 min by using a narrow-bore Xterra C(18) column (2.1 x 150 mm, 5 microm) with acetonitrile-(10 mmol/L ammonium acetate buffer, 0.1% acetic acid) (15:85, v/v). Ion signal m/z 330.1 for sinomenine was measured in the positive mode. Linearity was established for the range of concentrations of 2.0-10000.0 ng/mL with a coefficient of determination (r) of 0.9989. The intra- and inter-day reproducibility of the present method was better than 6%. The lower limit of quantification (LLOQ) was 1.0 ng/mL. The proposed method described provides more authentic information on pharmacokinetics and metabolism at the site of action by using the coupling of microdialysis to LC-ESI-MS technique than the traditional sampling methods.

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Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography–electrospray ionization ion trap tandem mass spectrometry

Cited by 80 publications

References 28 publications

Endogenous N -acyl taurines regulate skin wound healing

Endogenous N -acyl taurines regulate skin wound healing

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

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