Determination of carbohydrates in fruit juices by capillary electrophoresis and high-performance liquid chromatography

Klockow, Antje; Paulus, Aran; Figueiredo, Verena; Amadò, Renato; Widmer, Hans

doi:10.1016/0021-9673(94)80067-7

Cited by 88 publications

(28 citation statements)

References 29 publications

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“…The concentration detection limits are at least two orders of magnitude lower than those obtained in CE by indirect UV detection [29][30] or borate complexation [27]. In comparison to endcolumn electrochemical detection, an advantage in concentration limits is generated by the larger capillary diameters allowed.…”

Section: Separation Conditionsmentioning

confidence: 93%

“…However, due to their lack of strong chromophore or fluorophore, carbohydrates can not be detected directly with optical methods. To avoid sometimes complicated precolumn derivatization procedures [24][25][26], oncolumn complexation with borate and detection at 195 nm [27] or indirect fluorescence [28] or UV detection [29][30] has to be applied. These methods suffer from poor selectivity and high concentration detection limits, which are in the order of 10 -4 mol 1 -~ In liquid chromatography pulsed amperometric detection (PAD) with gold or platinum electrodes [31] has become a routine method for the determination of sugars.…”

Section: Introductionmentioning

confidence: 99%

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Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes

Huang

Kok

1995

Journal of Chromatography A

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Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes Huang, X.; Kok, W.T. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractThe separation by capillary electrophoresis and off-column amperometric detection of underivatized sugars has been studied. A palladium-metal union was used as grounded cathode to decouple the high separation voltage before detection. With a second piece of capillary the field decoupler was connected to a home-made T-shaped detection cell. Cuprous oxide modified microelectrodes were prepared and evaluated. For the detection of sugars they were operated at a constant potential of + 0.60 V. The peak-height reproducibility with these electrodes over a period of 18 h (25 injections) was within 7% absolutely and within 4% when one of the analytes was regarded as internal standard.To preserve the flat electroosmotic flow profile in the separation capillary, pressure compensation was applied. The contribution of various sources to the system zone variance was studied. Under optimized conditions plate numbers of over 100 000 were obtained.For the baseline separation of a mixture of sugars 0.10 mol I-' sodium hydroxide solutions had IO be used as background electrolyte. Detection limits of l-2 pmol I-' were found for various carbohydrates.

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Section: Separation Conditionsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes

Huang

Kok

1995

Journal of Chromatography A

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“…When a non-absorbing analyte, which carries the same charge as the marker ion, passes the detection window, the concentration of the marker ion will be decreased and, thus, a negative peak will be recorded. Inherently negatively charged molecules displace more chromophores in the operating buffer than neutral sugars charged at high pH and, thus, can be detected at the highest sensitivity (Klockow et al, 1994;Lu and Westerlund, 1996;Xu et al, 1995;Damm and Overklift, 1994;Hauri et al, 1996;Richmond and Yeung, 1993). Electrochemical detection is based on the measurement of current resulting from the oxidation or reduction of analytes at the surface of an electrode cell.…”

Section: Detection Of Underivatized Glycoconjugatesmentioning

confidence: 98%

State-of-the-art of capillary electrophoresis with application to the area of glycoconjugates

Karamanos

Lamari

1999

Biomed. Chromatogr.

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Glycoconjugates constitute a major group of biomolecules, which participate in several biological functions and processes. Their carbohydrate components play key roles in determining the properties of glycoconjugates and, therefore, analysis and structural characterization of carbohydrates are essential. Capillary electrophoresis, due to its high resolving power and sensitivity, has been successfully used for the analysis of carbohydrates. In this review the principles of high‐performance capillary electrophoresis; mechanisms employed for glycoconjugate analysis as well as the various detection techniques used are summarized. Copyright © 1999 John Wiley & Sons, Ltd.

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“…The method was validated with the use of a set of synthetic samples containing mixtures of the reducing sugars, and then it was applied to several commercial food products to demonstrate general applicability. Compared with the reported methods for simultaneous determination of glucose, fructose and lactose (14)(15)(16)(18)(19)(20), this method offers the potential advantages of high sensitivity, simplicity and rapidity for glucose, fructose and lactose determination.…”

Section: Introductionmentioning

confidence: 94%

“…An HPLC method with an electrochemical detector has also been used to separate and determine reducing sugars (15). A capillary zone electrophoresis method with indirect UV detection was adapted for the routine determination of carbohydrates in a variety of fruit juices (16). Because reducing sugars have no obvious UV-vis absorption or fluorescence properties, postcolumn derivatization is necessary.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of glucose, fructose and lactose in food samples using a continuous‐flow chemiluminescence method with the aid of artificial neural networks

2007

Luminescence

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In this study, a simple continuous-flow chemiluminescence (CL) system was developed for simultaneous determination of glucose, fructose and lactose in ternary mixtures of reducing sugars without previous separation. This method was based on the different kinetics of the individual sugars in the oxidation reaction with potassium ferricyanide. The known luminol-K(3)Fe(CN)(6) CL system was used to measure the kinetic data of the system. The CL intensity was measured and recorded every second from 1 to 300 s. The data obtained were processed chemometrically using an artificial neural network. The relative standard errors of prediction for three analytes were <5%. The proposed method was successfully applied to the simultaneous determination of the three sugars in some food samples.

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Determination of carbohydrates in fruit juices by capillary electrophoresis and high-performance liquid chromatography

Cited by 88 publications

References 29 publications

Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes

Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes

State-of-the-art of capillary electrophoresis with application to the area of glycoconjugates

Simultaneous determination of glucose, fructose and lactose in food samples using a continuous‐flow chemiluminescence method with the aid of artificial neural networks

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