Determination of carbofuran and its toxic metabolites in animal tissue by gas chromatography of their N-trifluoroacetyl derivatives

Wong, Lyle; Fisher, Frank M.

doi:10.1021/jf60198a018

Cited by 36 publications

(15 citation statements)

References 6 publications

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“…We hypothesized that the enzyme-linked surface treated with carbofuran would not react with ACh, which would then induce a uniform orientation of 5CB because of the lack of topographical change. We incubated the AChE-linked surface with carbofuran dissolved in acetonitrile and DI water in a 1:9 [20,28]. The incubation time of the inhibitor was 25 min to minimize any effects of irreversible reaction, prevent detachment of the inhibitor from the active site of enzyme, and to save detection time [5].…”

Section: Detection Of the Ache Inhibitor Carbamate Insecticide Usinmentioning

confidence: 99%

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

Han

Jang

2015

Colloid Polym Sci

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In this study, we report on a label-free biosensor for the detection of acetylcholine (ACh) and an acetylcholinesterase (AChE) inhibitor, utilizing liquid crystals (LCs) on a polymeric surface with periodic nanostructures. The polymeric nanostructures, which hold sinusoidal anisotropic patterns, were fabricated by a sequential process of poly(dimethylsiloxane) buckling and replication of these patterns on a poly(urethane acrylate) surface where a film of gold was deposited. AChE was then covalently immobilized onto the gold surface. Optical images of nematic 4-cyano-4′-pentylbiphenyl (5CB) revealed that it aligned parallel to the plane of the enzymedecorated surface. However, AChE-mediated hydrolysis of ACh resulted in a plume of choline, acetate, and unreacted ACh, which in turn produced a distinctive optical transition of 5CB (from uniform to random) by masking the anisotropic surface topography. The hydrolysis of ACh was inhibited in the presence of a carbamate insecticide (AChE inhibitor), which prevented the orientational transition of 5CB by decreasing the enzymatic activity of AChE. These results suggest that this LC-based enzymatic sensor is highly sensitive to ACh and the AChE inhibitor, with a detection limit of 10 and 1 nM, respectively. This study demonstrates that polymeric surfaces with continuous wavy features can be used as LCbased biosensors to amplify the existence of ACh and an AChE inhibitor without labeling or an additional amplification strategy.

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Section: Detection Of the Ache Inhibitor Carbamate Insecticide Usinmentioning

confidence: 99%

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

Han

Jang

2015

Colloid Polym Sci

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“…Indiscriminate use of carbofuran for controlling the insecticides and also leads to serious environmental problems. Spectrophotometry 4-17 GLC 18-22 GC [23][24][25][26] and HPLC [27][28][29] methods have been reported for the determination of carbofuran. The above reported methods suffers from several disadvantages such as less stability, pH dependent, poor sensitivity and most of them required specific solvents for the extraction of colour derivatives.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Determination of Carbofuran by Spectrophotometer using 4, 4‐azo‐bis‐3, 3′5, 5′‐tetra bromoaniline in various Environmental Samples

Bhargavi

Kiran

Kanchi

et al. 2006

Journal of Chemistry

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A simple and sensitive spectrophotometric technique was developed for the determination of carbofuran in its formulations, water and grain samples. The method was based on the alkaline hydrolyzed product of carbofuran phenol interacted with diazonium salt of 4,4-azo-bis-3,3′5,5′-tetra bromo aniline. The maximum absorbance of the red coloured derivative was measured at 470 nm. The beer’s law was obeyed in the concentration range of 0.1-16.0 µg/mL. The interference of the non target species were studied on the determination of carbofuran which increases the selectivity of the method. The present method was successfully applied for the determination of carbofuran in its formulations, water and grain samples.

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“…Один из основных метаболитов карбосульфана в организме теплокровныхкарбофуран (2,3-дигидро-2,2-диметилбензофуран-7-ил-N-метилкарбамат). Он самостоятельно применяется как инсектицидный протравитель системного типа против почвообитающих и наземных вредителей [1,2,5]. Карбофуран имеет вид бесцветных кристаллов или белого кристаллического порошка, его температура плавления 153-154 °С [1,2,5].…”

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“…По данным литературы [3,5], с увеличением времени, прошедшего с момента отравления карбосульфаном, его концентрация снижается, а в тканях и биологических жидкостях может накапливаться карбофуран. Вопросы распределения карбосульфана и карбофурана в органах и биожидкостях теплокровных организмов до настоящего времени остаются малоизученными.…”

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“…Судебно-медицинСкая экСпертиза, 5,2015 ных групп по 5 особей в каждой) с массой 180-230 г через зонд вводили в желудок анализируемое вещество в количестве 420 мг на 1 кг массы животного (тройная ЛД 50 ) в виде водной эмульсии однократно. После гибели животных трупы вскрывали, одинаковые органы и биожидкости, взятые от животных в каждой группе, объединяли и исследовали на наличие в них карбосульфана и его метаболита карбофурана.…”

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The distribution of carbosulfan in the organism of the warm-blooded animals

Vk¹,

Sg²,

Ap³

2015

Sud.-med. ekspert.

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The objective of the present work was to study the specific distribution patterns of carbosulfan in the body of warm-blooded animals by means of thin-layer chromatography, low-pressure column chromatography, electron spectrophotometry, and GCh/ MS. The carbosulfan distribution in the warm-blooded animals (rats) was investigated after the intragastric administration of this poisonous substance at a dose equivalent to three median lethal doses (LD50) in the form of an aqueous emulsion. The highest concentrations of carbosulfan were found in the contents of the stomach, small and large intestines, liver, and lungs. The same organs and spleen contained carbofuran, the main metabolite of carbosulfan.

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Determination of carbofuran and its toxic metabolites in animal tissue by gas chromatography of their N-trifluoroacetyl derivatives

Cited by 36 publications

References 6 publications

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface

A Sensitive Determination of Carbofuran by Spectrophotometer using 4, 4‐azo‐bis‐3, 3′5, 5′‐tetra bromoaniline in various Environmental Samples

The distribution of carbosulfan in the organism of the warm-blooded animals

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