Determination of Carbamazepine in Uncoated and Film-Coated Tablets by HPLC with UV Detection

Abstract

A simple and reliable method for the determination of carbamazepine in uncoated and film-coated tablets has been developed and validated by high-performance liquid chromatography with ibuprofen as the internal standard. The tablets were weighed and ground individually, and were extracted in methanol using method of sonification. The extract was diluted and purified using 0.45 mm MARCEL DEKKER, INC. • 270 MADISON AVENUE • NEW YORK, NY 10016membrane filter. Separation was achieved on an Econosphere C18 5m analytical column with a mobile phase of 28:72 acetonitrile:sodium phosphate buffer. Detection was at UV 230 nm using a photodiode array detector. The mean retention times of carbamazepine and ibuprofen were 4.2 and 12.3 min, respectively. Peak height ratios were fit to a least squares linear regression for calculation of standard regression equation. The linear calibration range was from 5.0-25.0 mg/mL with the lower limit of detection (LLOD) of 18 ng/mL and the lower limit of quantitation (LLOQ) of 60 ng/mL. This simple and convenient method produced good linearity, precision, and accuracy and avoids using methylene chloride as the mobile phase. A modified USP method was also carried to analyze the drug content in the tablets. These results were compared statistically, and no difference was found between the new method and the modified USP method, while the new method is more convenient and is suitable for stability and dissolution studies of this drug.

A pectin/chitosan/zinc oxide nanocomposite for adsorption/photocatalytic remediation of carbamazepine in water samples

“…1,2 CBZ is a tricyclic neutral compound of lipophilic nature and is utilized in the treatment of trigeminal neuralgia, partial epilepsy and as an adjunct therapy in psychosis. 4 Various routes of treatment have been proposed to overcome the inefficiency of WWTP in the removal of CBZ. Such approaches include advanced oxidation, [5][6][7][8][9][10] membrane ltration, [11][12][13] and ozonation.…”

Spectrophotometric Determination of Carbamazepine in Pharmaceutical Formulations Based on its Reaction with a Brominating Agent

“…The literature regarding the processes for the specification of CBZ in biological materials using different techniques is wide: LC (Džodić et al, 2009;Walker, 1988), flow injection (FI) spectrophotometry (Çomoğlu et al, 2006), FI chemiluminescence (CL) (Xiong et al, 2009), Gas chromatography (GC) (Liu et al, 1991;Kadioglu and Demirkaya, 2007), HPLC (Panchagnula et al, 1998;Yuan et al, 2003;Demirkaya and Kadioglu, 2005;Demirkaya and Kadioglu, 2008;Ulu, 2006), polarography (Zhang et al, 1993), FI-spectrofluorimetry (Huang et al, 2002), CL (Lee et al, 2003;Abed and Al-Abachi, 2015), and visible spectrophotometry (Rao and Murty, 1982;Agrawal et al, 1989). citrate.…”

Development and Validation of a Rapid Stability-Indicating HPLC Method for Determination of Carbamazepine in Pure and Dosage Forms

“…Carbamazepine is official in british pharmacopeia 2 as it was determined by liquid chrpomatographic (LC) method. Various techniques have been reported for the assay of CBMZ in pharmaceutical dosage forms and biological fluids, including high-performance liquid chromatography (HPLC) with ultra violet or photo diode detection [3][4][5][6][7][8][9][10][11][12] , high-performance thin layer chromatography (HPTLC) 13 , LC-mass spectrometry methods [14][15][16] , fluorescence polarization assay (FPA) 17,18 , gas chromatography with mass spectrometry [19][20][21] , micellar electro kinetic capillary chromatography (MECC) 22 , chemiluminescence 23,24 , spectrofluorimetry 25 , FT-Raman spectroscopy 26 , flow-injection 27 and spectrophotometry [28][29][30][31][32][33][34][35][36][37] have been reported for the detection of CBMZ and its metabolites.…”