Determination of carbamazepine and carbamazepine 10,11-epoxide in human plasma by tandem liquid chromatography–mass spectrometry with electrospray ionisation

Liquid chromatography–electrospray mass spectrometry determination of carbamazepine, oxcarbazepine and eight of their metabolites in human plasma

“…To date, some HPLC methods with ultra-violet [17][18][19][24][25][26][27][28][29][30][31][32][33][34] or mass spectrometry [10,11,35,36] detection for determining CBZ and/or OXCBZ in biological fluids (plasma, urine) have been published. Some of them involved both the quantitation of CBZ or OXCBZ and their main metabolites (CBZ-EP, 10-OH-CBZ, DiOH-CBZ [19,24,[26][27][28][29][30][31]33,34].…”

“…Four liquid chromatography-mass spectrometry (LC-MS) methods have been published; two of them were focused mainly to the determination of the 9-acridan [10,11]. Two methods have recently been described, the first one for the determination of CBZ and CBZ-EP by tandem liquid chromatography-mass spectrometry with electrospray ionisation [35] and the second one for the determination of CBZ and 10-OH-CBZ by atmospheric pressure chemicalionization liquid chromatography/mass spectrometry [36]. All these methods involved tedious sample pretreatment (liquid-liquid or solid-phase extraction) of the biological samples.…”

Determination of the psychoactive drugs carbamazepine and diazepam in hospital effluent and identification of their metabolites

“…The rupture occurs in the carboxamide side chain with the loss of a NH 2 group, which leads to the formation of m/z 236; this is followed by a further rupture of the structure caused by a loss of 2 CO, and forms the m/z 180 fragment; and finally, by ring opening and loss of CH, which forms m/z 167, as confirmed by Miao and Metcalfe (2003) and Bahlmann et al (2014). Other researchers have also found these fragments in their experiments, such as Rooyen et al (2002), Breton et al (2005) and Niessen (2011), who detected this metabolite in human plasma. A second route proposed was the formation of the fragment (m/z 210) through the loss of the NCO group, which subsequently loses the H 2 CO cluster, and forms the m/z 180 fragment.…”

Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

“…There are several methods applying liquid chromatography (LC) with ultraviolet detection (LC-UV), [3][4][5][6] LC coupled to a single mass spectrometer detector (LC-MS) 2 and in tandem MS (LC-MS/ MS), 7,8 mixed micellar LC, 9 micellar electrokinetic capillary chromatography (MEKC), 10 capillary electrophoresis (CE), 11 gas chromatography coupled to MS (GC-MS), 12,13 and GC coupled to thermionic detector (GC-TSD).…”