Determination of captopril in plasma by high-performance liquid chromatography for pharmacokinetic studies

Arroyo, Carmen M.; López-Calull, C; Garcı́a-Capdevila, L; Gich, Ignasi; Barbanoj, M.J.; Bonal, Joaquin

doi:10.1016/s0378-4347(96)00306-4

Cited by 24 publications

(15 citation statements)

References 10 publications

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“…[1][2][3] Different analytical methods have been applied to the determination of the remaining drugs analyzed by us, and also by HPLC techniques. Many of the HPLC analytical methods exist for the assay of ENA, [4][5][6][7] SOT, [8][9][10][11][12][13][14][15] CAP, [16][17][18][19][20][21][22][23][24] MET, [25][26][27][28][29][30][31] 62,63 and SPI [64][65][66][67][68] in different biological fluids and tissues. HPLC methods were applied to the simultaneous quantification of selected β-blockers (also: SOT, MET and PRO) in human fluids (plasma, serum, urine).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

2009

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We have developed and validated a new and reliable gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method with a diode array detector (DAD) for the simultaneous separation and determination of 23 frequently prescribed selected drugs belonging to different therapeutic groups in human urine samples. For the drugs listed below, this method of analysis for human urine was also successfully applied to determine urine concentrations of these drugs in samples from treated patients: enalapril (ENA), paracetamol (PAR), sotalol (SOT), dipyrone (DIP), vancomycin (VAN), captopril (CAP), fluconazole (FLU), cefazolin (CEF), metoprolol (MET), aspirin (ASP), ticlopidine (TIC), prednisolone (PRE), propranolol (PRO), digoxin (DIG), sildenafil (SIL), furosemide (FUR), dexamethasone (DEX), carvedilol (CAR), ketoprofen (KET), nifedipine (NIF), terbinafine (TER), acenocoumarol (ACE) and spironolactone (SPI). Separation of the analytes was achieved by RP-HPLC-DAD with a mobile phase composed of acetonitrile, methanol and 0.05% trifluoroacetic acid in water using a gradient elution program. Good linear relationships over the investigated concentration ranges were observed with values of r 2 higher than 0.998 for all of the drugs. The intra-day and inter-day precisions of this method were evaluated with RSD values less than 4.26 and 5.42%, respectively. The relative recoveries of the 23 investigated compounds ranged from 93.60 to 106.00% with RSD values less than 4.46%. An expanded uncertainty budget was constructed for all investigated drugs in human urine samples.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

2009

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“…As Tabelas 4 e 5 relacionam os fármacos que têm sido quantificados empregando métodos de separação como CLAE [161][162][163][164][165][166][167][168][169][170][171][172][173][174][175][176][177] (Tabela 4) e eletroforese capilar 50,[178][179][180][181] (Tabela 5) na análise de amostras complexas, como plasma e urina, ou na quantificação de misturas de fármacos e em estudos relacionados com bioequiva-lência, farmacocinêtica e biodisponibilidade. Desta forma, estas técnicas apresentam grande potencialidade de uso em situações específicas onde a matriz da amostra seja muito complexa.…”

Section: Emprego De Técnicas De Separação Com Detecção Fluorimétricaunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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Recebido em 20/10/06; aceito em 30/11/07; publicado na web em 4/9/08 APPLICATION AND ADVANCES IN THE LUMINESCENCE SPECTROSCOPY IN PHARMACEUTICAL ANALYSES. This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo-and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.Keywords: luminescence spectroscopy; drugs; cosmetics analysis; introdução Devido ao progresso contínuo em técnicas e instrumentos, a indústria farmacêutica busca o emprego de metodologias que ofereçam maior sensibilidade, seletividade, robustez, precisão, exatidão e rapidez. Neste sentido, um grande número de equipamentos de cromatografia líquida de alta eficiência (CLAE) com diversos tipos de detecção domina este ramo industrial. Contudo, o advento da sofisticação de instrumentação automatizada de espectrômetros de luminescência poderá resultar em um substancial aumento de aplicações desta técnica instrumental na indústria farmacêutica, principalmente, no que se refere à análise diretamente na matriz do medicamento.A espectroscopia de luminescência é uma ferramenta analítica extremamente sensível e tem sido amplamente aplicada na resolução de problemas que requerem baixos limites de detecção, podendo ser facilmente encontrados na literatura muitos trabalhos mostrando análises ao nível de traços.1-6 A sensibilidade inerente a esta técnica é consideravelmente maior em comparação a outras metodologias, entre elas a espectrofotometria UV/visível, apresentando limites de detecção de até três ordens de grandeza menores, os quais normalmente se encontram na faixa de ng mL -1 , sendo conseqüência do baixo sinal de fundo que as medidas fluorescentes apresentam. Diferentemente do que ocorre no fenômeno da luminescência, nas medidas de absorbância o sinal está associado à atenuação da intensidade de um feixe de radiação por uma espécie absorvente. Nos casos onde a quantidade da espécie é pequena, esta atenuação é menor, tornando assim a detecção da espécie difícil. Outra característica importante da luminescência diz respeito à seletividade, porque espécies com rendimentos quânticos de fluorescência apreciáveis são menos comuns do que substâncias cuja fluorescência não pode ser detectada. Desta forma, moléculas fluorescentes apresentam comprimento de onda característico de excitação e/ou emissão. Adicionalmente, o comprimento de onda de excitação d...

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“…1). NPM is one of the most popular fluorogenic reagents for the LC analysis of thiols used in both postcolumn [23][24][25][26] and precolumn reactions; [27][28][29][30][31] however, excimer fluorescence detection has not been carried out until now. Dimercaprol (BAL) and dithiothreitol (DTT) were used as model polythiols to optimize the separation and derivatization conditions.…”

Section: Introductionmentioning

confidence: 99%

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

et al. 2009

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An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440 -520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.

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Determination of captopril in plasma by high-performance liquid chromatography for pharmacokinetic studies

Cited by 24 publications

References 10 publications

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

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