Determination of Calcium Mobility in Alkaline‐Cooked Grounded Corn in Presence of a Magnetic Field

Vargas, Susana; Rangel, Domingo; Quintanilla, Francisco; Rodrı́guez, Rogelio

doi:10.1111/1750-3841.12421

Cited by 3 publications

(5 citation statements)

References 28 publications

(28 reference statements)

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“…We evaluated mitochondrial membrane potential in cultured fibroblasts from control and AD patients using TMRM (Figures 3A,B ; Brand and Nicholls, 2011 ; Vargas et al, 2014 ). No significant changes in mitochondrial potential levels were detected in patients with AD compared to age-matched controls in basal conditions (Figures 3A,B ).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, studies in AD cell models have been shown that mitochondrial potential is only affected under stressor conditions such as calcium or amyloid beta peptide (Quintanilla et al, 2012 ; Vargas et al, 2014 ). In that context, we found that in all analyzed patients showed equal levels of basal calcium (Figures 4A,B ), and after treatment with thapsigargin produced a significant increase in relative calcium levels in AD patients respect to control cells (Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…The mitochondrial potential was determined using the mitochondrial dye TMRM in non-quenching mode, as described previously with modifications (Quintanilla et al, 2013 ; Vargas et al, 2014 ). Mitochondrial potential levels were expressed as the average of fluorescence signal (F) per area in every image minus the intensity of background fluorescence (F0).…”

Section: Methodsmentioning

confidence: 99%

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Mitochondrial Bioenergetics Is Altered in Fibroblasts from Patients with Sporadic Alzheimer's Disease

et al. 2017

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The identification of an early biomarker to diagnose Alzheimer's disease (AD) remains a challenge. Neuropathological studies in animal and AD patients have shown that mitochondrial dysfunction is a hallmark of the development of the disease. Current studies suggest the use of peripheral tissues, like skin fibroblasts as a possibility to detect the early pathological alterations present in the AD brain. In this context, we studied mitochondrial function properties (bioenergetics and morphology) in cultured fibroblasts obtained from AD, aged-match and young healthy patients. We observed that AD fibroblasts presented a significant reduction in mitochondrial length with important changes in the expression of proteins that control mitochondrial fusion. Moreover, AD fibroblasts showed a distinct alteration in proteolytic processing of OPA1, a master regulator of mitochondrial fusion, compared to control fibroblasts. Complementary to these changes AD fibroblasts showed a dysfunctional mitochondrial bioenergetics profile that differentiates these cells from aged-matched and young patient fibroblasts. Our findings suggest that the human skin fibroblasts obtained from AD patients could replicate mitochondrial impairment observed in the AD brain. These promising observations suggest that the analysis of mitochondrial bioenergetics could represent a promising strategy to develop new diagnostic methods in peripheral tissues of AD patients.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Mitochondrial Bioenergetics Is Altered in Fibroblasts from Patients with Sporadic Alzheimer's Disease

et al. 2017

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“…The mitochondrial potential was determined using the mitochondrial dye tetramethyl-rhodamine methyl ester (TMRM) in non-quenching mode, as described previously with modifications [32] , [36] . Mitochondrial potential levels were expressed as the average of fluorescence signal (F) per area in every image, and then subtracted by the intensity of background fluorescence (F0).…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial permeability transition pore contributes to mitochondrial dysfunction in fibroblasts of patients with sporadic Alzheimer's disease

et al. 2018

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In the last few decades, many reports have suggested that mitochondrial function impairment is a hallmark of Alzheimer's disease (AD). Although AD is a neurodegenerative disorder, mitochondrial damage is also present in patients’ peripheral tissues, suggesting a target to develop new biomarkers. Our previous findings indicate that AD fibroblasts show specific defects in mitochondrial dynamics and bioenergetics, which affects the generation of adenosine triphosphate (ATP). Therefore, we explored the possible mechanisms involved in this mitochondrial failure. We found that compared with normal fibroblasts, AD fibroblasts had mitochondrial calcium dysregulation. Further, AD fibroblasts showed a persistent activation of the non-specific mitochondrial calcium channel, the mitochondrial permeability transition pore (mPTP). Moreover, the pharmacological blockage of mPTP with Cyclosporine A (CsA) prevented the increase of mitochondrial superoxide levels, and significantly improved mitochondrial and cytosolic calcium dysregulation in AD fibroblasts. Finally, despite the failure of CsA to improve ATP levels, the inhibition of mitochondrial calcium uptake by the mitochondrial calcium uniporter increased ATP production in AD fibroblasts, indicating that these two mechanisms may contribute to mitochondrial failure in AD fibroblasts. These findings suggest that peripheral cells present similar signs of mitochondrial dysfunction observed in the brain of AD patients. Therefore, our work creates possibilities of new targets to study for early diagnosis of the AD.

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“…A Keithley model 2400 source-meter was used to apply 2.0 V during the 30 s prior to the sample removal; the data acquisition system was a Textronic, model TX3 apparatus. 36,37 The same procedure was repeated for all of the samples. The relaxation of the voltage signal was monitored through the determination of the ionic current; Fig.…”

Section: A Sample Preparationmentioning

confidence: 99%

Determination of lead ion removal from a flowing electrolyte in the presence of a magnetic field using Raman spectroscopy

et al. 2015

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The results indicate that the ion concentration can be increased more than 80% in a region where they can be removed. The increment in the ion concentration produced by the deflection due to the magnetic field, together with the use of Raman spectroscopy, allows for a rapid analysis of the removed ions without any previous preparation. The proposed method is a potentially useful method for metal ion separation of interest in the medical physics field.

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Determination of Calcium Mobility in Alkaline‐Cooked Grounded Corn in Presence of a Magnetic Field

Cited by 3 publications

References 28 publications

Mitochondrial Bioenergetics Is Altered in Fibroblasts from Patients with Sporadic Alzheimer's Disease

Mitochondrial Bioenergetics Is Altered in Fibroblasts from Patients with Sporadic Alzheimer's Disease

Mitochondrial permeability transition pore contributes to mitochondrial dysfunction in fibroblasts of patients with sporadic Alzheimer's disease

Determination of lead ion removal from a flowing electrolyte in the presence of a magnetic field using Raman spectroscopy

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