Determination of calcium and magnesium in milk by complexometric titration using protein precipitation and complexation with Palladiazo or other indicators

Jiménez, M. Dolores Alvarez; Gil, Manjae; Corvillo, María Antonia Palacios; Díez, L. M. Polo

doi:10.1039/an9881300633

Cited by 11 publications

(6 citation statements)

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“…This value is also higher than the WHO threshold value of 30 mg/100 mL. The value obtained in this study is higher than 17.6-25.0 mg/100 mL value of previous work reported in literature [12]. Some factors that may be responsible for these differences include nutritional status, age of lactating mothers and health status of mother during pregnancy period.…”

Section: Resultscontrasting

confidence: 75%

“…Analytical method used in this study is also suggested to be a major factor in that the human milk samples were deproteinized before preconcentration with complexing agent which were not used in the one reported in literature [16]. The magnesium content of the human milk was also determined to be in the range of 18.95-25.75 mg/100 mL and is higher than the WHO value of 7.0 mg/100 mL [12]. The above factors enumerated for calcium content could also be applied for the difference in magnesium content concentration.…”

Section: Resultsmentioning

confidence: 98%

“…This was made up to 100 mL mark of the standard volumetric flask by washing residue obtained with 2% w/v salicylic acid. This process removed protein from the mixture and has been fully described elsewhere [12]. The pH of the clear filtrate was adjusted to 5 with 2 M NaOH using Kent EIL 7055 pH meter.…”

Section: Methodsmentioning

confidence: 99%

“…Prior to pre-concentration process, deproteination of milk is necessary. Trichloroacetic and salicylic acids have been reported to be effective as deproteination agents [12]. The use of ammonium pyrrolidinedithiocarbamate-methylisobutyl ketone extraction system has been reported for the determination of Ag, Cd, Co, Cr, Cu, Fe, Ni, Mn.…”

Section: Introductionmentioning

confidence: 99%

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<b>Comparison of some preconcentration methods for certain metal ions in human milk and yogurts</b>

Ibitoye

2003

Bull. Chem. Soc. Eth.

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Five samples each of yogurts and human milk were separately collected, deproteinized, pre-concentrated differently with 1% 8-hydroxyquinoline, 1% ammonium pyrrolidine-dithiocarbamate (APDC) and extracted with methyl-isobutylketone (MIBK). Activated carbon powder in HNO 3 was also used. The extracts obtained were analyzed using atomic absorption spectrometry for some metal ions. In yogurts, the powdered activated carbon in HNO 3 exhibited the highest complexing ability with values of 0.

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Section: Resultscontrasting

confidence: 75%

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<b>Comparison of some preconcentration methods for certain metal ions in human milk and yogurts</b>

Ibitoye

2003

Bull. Chem. Soc. Eth.

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“…We used the vital fluorescent bone marker calcein in order to characterize bone growth, which was widely used in the fluorescent analysis to determine many metal ions and as an indicator for titration analysis (Diehl and Ellingboe, 1956;Wallach et al, 1959;Jiménez et al, 1988). Twelve rats in each group had 20 mg/kg calcein (Sigma, US) injected into the dorsal subcutaneous area every two weeks.…”

Section: Fluorescent Labelingmentioning

confidence: 99%

Effects of different types of palatal lateral excisions on growth and development of maxilla and dental arch

Zheng

Shi

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:Objective: This study aimed to explore the effects of different types of palatal lateral excisions on the growth and development of the maxilla and dental arch, and to investigate the underlying mechanisms. Methods: A total of 112 3-week-old Sprague-Dawley (SD) male rats were randomly divided into a control and 3 experimental groups: the mucoperiosteal denudation group, the mucosal flap excision group, and the periosteum excision group. In the experimental groups, bilateral mucoperiosteal, mucosal flap and periosteum were excised respectively in the lateral one half of the palate. Four rats in each group were randomly chosen for sacrifice every two weeks. The maxilla was dissected following the excision. The widths of the maxilla and dental arch were measured and the histological phenomena were investigated at different phases. At the same time, 12 animals in each group were sequentially injected with calcein every two weeks. Three animals in each group, whose fluorescent labeling was used, were sacrificed for investigating bone formation at Week 8 following injection. Results: (1) Each experimental group presented the constriction of the maxilla and dental arch. The upper first molars in the experimental groups inclined medially. The mucoperiosteal denudation group showed the largest degree of effect followed by the periosteum excision group. The indices of the mucosal flap excision group, which retained the structures of the periosteum layer, had the most approximate values to the control group; (2) Different histological changes among the experimental groups were detected. The fibers penetrated into the palatal bone as Sharpey's fibers in the mucoperiosteal denudation group. The pattern of bone deposition was the bundle type. Sharpey's fibers were not found in the mucosal flap and periosteum excision groups and the depositions of palatal bone were the lamellar type as those in the control group; (3) The rates of bone deposition in the experimental groups decreased compared with the control group. The rates in different phases were the most approximate values to those of the control group in the mucosal flap excision group, which has the same structure of periosteum as the control group. Conclusion: There were different effects on the growth and development of the maxilla and dental arch in different types of palatal lateral excisions. Periosteum is important for bone formation and deposition pattern. The prevention of Sharpey's fibers forming and attaching to the palatine can effectively avert the following malformation.

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Novel chemiluminescence system with calcein as chemiluminescent reagent

Nie

2007

Luminescence

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Chemiluminescence (CL) was observed when calcein reacted with potassium ferricyanide. Several metal ions and organic compounds could enhance this CL signal. The possible mechanisms for the CL reactions and the enhancement effect were investigated via studies of the CL kinetic characteristics, the CL spectra of some related reactions, the UV absorption spectra and the fluorescence spectra of some substances. The merits of figures for some substances with CL activity were obtained. A new CL system with calcein as the CL reagent was preliminarily established.

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Determination of calcium and magnesium in milk by complexometric titration using protein precipitation and complexation with Palladiazo or other indicators

Cited by 11 publications

References 0 publications

<b>Comparison of some preconcentration methods for certain metal ions in human milk and yogurts</b>

<b>Comparison of some preconcentration methods for certain metal ions in human milk and yogurts</b>

Effects of different types of palatal lateral excisions on growth and development of maxilla and dental arch

Novel chemiluminescence system with calcein as chemiluminescent reagent

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