Determination of buprenorphine in plasma by liquid chromatography: application to heroin-dependent subjects

Lagrange, F.; Péhourcq, Fabienne; Baumevieille, Marie; Bégaud, Bernard

doi:10.1016/s0731-7085(97)00139-8

Cited by 20 publications

(11 citation statements)

References 21 publications

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“…Short-and long-term stability studies on these analytes have been limited to buprenorphine (20,28,29). Current stability experiments focused on storage conditions that are relevant to the day-to-day analyses of these compounds.…”

Section: Stabilitymentioning

confidence: 99%

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A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

Moody

Slawson

Strain

et al. 2002

Analytical Biochemistry

123

101

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Section: Stabilitymentioning

confidence: 99%

“…These studies provide limits for storage under these conditions. Subsequent experiments may expand these limits; for example, Lagrange et al (28) have shown that buprenorphine is stable in plasma stored at 4°C for 8 days.…”

Section: Stabilitymentioning

confidence: 99%

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

Moody

Slawson

Strain

et al. 2002

Analytical Biochemistry

123

101

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“…25 Since our samples were relatively pure compared with plasma samples, the method was slightly modified to shorten the retention time for buprenorphine. In short, the chromatographic system consisted of a Waters Model 600 programmable solvent delivery module, a Waters Model 717plus auto sampler, and a Waters Model 996 photodiode array detector (Waters, Milford, MA).…”

Section: Hplc Analysismentioning

confidence: 99%

In vitro dissolution method for evaluation of buprenorphine in situ gel formulation: A technical note

2007

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“…Different methods, including liquid chromatography with UV [11][12][13], fluorescence [4,14], electrochemical [15][16][17] and mass spectrometric [18][19][20] detection, have been applied to the determination of BUP and its metabolites in plasma, serum, whole blood, urine, feces, tissues, hair and sweat patches. In healthy subjects, the maximum plasma concentration (C max ) of buprenorphine and norbuprenorphine was 3.2 ng mL -1 and 0.87 ng mL -1 after a single 8 mg/2 mg dose of buprenorphine/naloxone [21].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma by LC-MS-MS

Chiang

Pao²,

Hsiong³

et al. 2011

Chromatographia

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A novel sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method simultaneously determined buprenorphine (BUP) and its active metabolite, norbuprenorphine (NBUP), and a coformulant, naloxone was developed, validated and applied successfully in humans. Buprenorphine-d 4 and norbuprenorphine-d 3 were used as the internal standard. The analysis was performed on a silica column, and the mobile phase was isocratic and composed of acetonitrile:2 mM ammonium formate in H2O (82:18, v/v). Mass spectrometry employed multiple reaction monitoring modes with transitions of m/z 468.1-55.2 for BUP, 414.2-101.2 for NBUP, 328.3-310.3 for naloxone, 472.1-59.2 for buprenorphine-d 4 and 417.2-101.2 for norbuprenorphine-d 3 . Lower limit of quantification (LLOQ) of the analytical method was 0.05 ng mL -1 for BUP, 0.1 ng mL -1 for NBUP and 0.025 ng mL -1 for naloxone, respectively. The standard calibration curves of BUP, NBUP and naloxone were linear over the concentration range of 0.05-20 ng mL -1 , 0.1-20 ng mL -1 and 0.025-20 ng mL -1 , respectively. The precisions (RSD) and accuracies (RE) of LLOQ and other QC samples were in acceptable range, with RSD \ 20% and RE ± 20% for LLOQ and RSD \ 15% and RE within ±15% for QC samples. The method was accurate, precise and specific, and was applied to the pharmacokinetic study of buprenorphine in healthy volunteers.

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Determination of buprenorphine in plasma by liquid chromatography: application to heroin-dependent subjects

Cited by 20 publications

References 21 publications

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

In vitro dissolution method for evaluation of buprenorphine in situ gel formulation: A technical note

Simultaneous Determination of Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma by LC-MS-MS

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