Determination of Buprenorphine in Human Plasma by Gas Chromatography-Positive Ion Chemical Ionization Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry

Moody, David E.; Laycock, John D.; Spanbauer, Alan C.; Crouch, Dennis J.; Foltz, Rodger L.; Josephs, Jonathan L.; Amass, Leslie; Bickel, Warren K.

doi:10.1093/jat/21.6.406

Cited by 67 publications

(40 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following up to 8 mg sublingual buprenorphine, peak plasma concentrations rarely exceed 10 ng/ml (0.021 M) (16 -18) and can reach subnanogram-per-milliliter concentrations within 24 h of even higher doses (19,20). It is therefore important that sensitive methods be employed for pharmacokinetic and safety studies on buprenorphine.…”

Section: Elsevier Science (Usa)mentioning

confidence: 99%

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

Moody

Slawson

Strain

et al. 2002

Analytical Biochemistry

123

101

View full text Add to dashboard Cite

Section: Elsevier Science (Usa)mentioning

confidence: 99%

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

Moody

Slawson

Strain

et al. 2002

Analytical Biochemistry

123

101

View full text Add to dashboard Cite

“…This very low concentration level constitutes a challenging task for analysis. 5 There were various analytical methods for BP in biological specimens, such as gas chromatography (GC), 6 gas chromatography-mass spectrometry (GC-MS), [8][9][10] high-performance liquid chromatography(HPLC) 7 and high-performance liquid chromatography-mass spectrometry (HPLC-MS).…”

Section: Introductionmentioning

confidence: 99%

“…But many methods had no reported assay validation or incomplete assay validation, and could not perform simultaneous determination of BP, NBP and NLX in human plasma. 1,[3][4][5]9 A rapid and simultaneous method for determination of BP, NBP and NLX in human plasma by UPLC-MS/MS was established. The method optimized the parameters of SPE and other UPLC-MS/MS parameters, so that can get the good recoveries and little interference.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid and Simultaneous Detection Method for Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma Using Ultra‐high Performance Liquid Chromatography‐tandem Mass Spectrometer with Solid‐phase Extraction

Guo

2011

Chin. J. Chem.

View full text Add to dashboard Cite

A simultaneous method was successfully established and validated for the separation and determination of buprenorphine (BP), its primary metabolite, nor-buprenorphine (NBP) and a proposed co-formulate, naloxone (NLX) in human plasma. The method used buprenorphine-d 4 (BP-D 4 ), nor-buprenorphine-d 3 (NBP-D 3 ), naltrexone (NTX) as internal standards (ISs). 100 µL of plasma sample fortified with the ISs was cleaned up by solid-phase extraction (SPE), and was then separated on a Waters Acquity TM BEH C 18 column with gradient elution using methanol and water (containing 0.2% formic) at a flow rate of 0.25 mL•min -1 . The mass spectrometer was used for detection and was operated in the positive electrospray ionization with multiple reaction monitoring (MRM) mode. The three compounds were effectively separated in 5 min. The linear ranges of the compounds were 0.1-25, 0.25-25 and 0.05-25 ng•mL -1 for BP, NBP and NLX, respectively, with r≥0.9935. The method had high sensitivity (the limits of detection were 0.02, 0.1 and 0.01 ng•mL -1 for BP, NBP and NLX, respectively) and high recoveries (≥97.6%). The result was shown to be linear and satisfactorily met current acceptance criteria for validation of bioanalytical method: intra and inter assay precisions within the required limits of ≤25% RSD. The LOQs fulfilled the LOQ requirements: precision≤25% RSD, and was fully validated according to the State Food and Drug Administration (SFDA) regulations. The results demonstrated that ultra-high performance liquid chromatographytandem mass spectrometer (UPLC-MS/MS) with SPE was a powerful detection tool and contributed to pharmaceutical analysis in biological matrices.

show abstract

“…Many chromatographic techniques were proposed for the determination of BU and its metabolite norbuprenorphine (NBU) in biological matrices, from HPLC with coulometric detection (12), liquid chromatography-mass spectrometry coupling (LC-MS) (13,14), and gas chromatography -mass spectrometry (GC-MS) (12,14). Mass spectrometry is very often used as it fulfills the requirement of specificity necessary for forensic investigations (unexplained deaths, buprénorphine abuse, etc.…”

Section: Introductionmentioning

confidence: 99%

“…), and because of its high sensitivity, very useful for the determination of this low-concentration drug. Most of the methods employing mass spectrometry yielded LLOQ between 0.1 ng/ml (13) and 0.5 ng/ml (14). One peculiarity of treatments with HD-BU is that their efficacy can be checked using toxicological analyses, most often by screening urine samples for drugs of abuse using immunoassays then confirming the positive samples with mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic drug monitoring of high-dose buprenorphine : why and how ?

Marquet

Kintz²

2004

Ann Toxicol Anal

View full text Add to dashboard Cite

Determination of Buprenorphine in Human Plasma by Gas Chromatography-Positive Ion Chemical Ionization Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry

Cited by 67 publications

References 23 publications

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

A Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometric Method for Determination of Buprenorphine, Its Metabolite, norBuprenorphine, and a Coformulant, Naloxone, That Is Suitable for in Vivo and in Vitro Metabolism Studies

Development of a Rapid and Simultaneous Detection Method for Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma Using Ultra‐high Performance Liquid Chromatography‐tandem Mass Spectrometer with Solid‐phase Extraction

Therapeutic drug monitoring of high-dose buprenorphine : why and how ?

Contact Info

Product

Resources

About