Determination of bromazepam and its urinary metabolites, with a previous hydrolysis reaction, by voltammetric and spectrophotometric techniques

Valdeón, Josefa Lopez; Sevilla, Ma Teresa; Hernández, L.

doi:10.1039/an9871201365

Cited by 14 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These reported methods also reveal many analytical techniques for BMZ determination either alone or together with other benzodiazepines, in both pharmaceutical or biological matrices. These techniques include HPLC [13][14][15][16][17][18][19][20][21][22][23][24], LC-MS [25,26], GC-MS [27], TLC [28], capillary electrophoresis [29], spectrophotometry [30][31][32], ion-selective electrode [33][34][35], potentiometric [36] and voltammetric [37,38] methods.…”

Section: Introductionmentioning

confidence: 99%

“…Over the past decades, several publications showed an interest in stability studies of BMZ, especially degradation of the drug due to hydrolysis, which cause the formation of the 2-(2-amino-5-bromobenzoyl) pyridine (ABP), Fig 1 [18,39,40]. Beside the BP [7] defines 2-(2-amino-5-bromobenzoyl) pyridine (ABP) as a possible impurity in pure bromazepam powder, the main metabolic pathway of bromazepam includes C3 hydroxylation and heterocyclic ring hydrolysis producing two metabolites 3-hydroxy bromazepam and ABP metabolites that excreted in the urine [38,41,42].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of a stability indicating RP-HPLC-DAD method for the determination of bromazepam

Darwish

Ali²,

Naguib

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

A reliable, selective and sensitive stability-indicating RP-HPLC assay was established for the quantitation of bromazepam (BMZ) and one of the degradant and stated potential impurities; 2-(2-amino-5-bromobenzoyl) pyridine (ABP). The assay was accomplished on a C18 column (250 mm × 4.6 mm i.d., 5 μm particle size), and utilizing methanol-water (70: 30, v/v) as the mobile phase, at a flow rate of 1.0 ml min-1. HPLC detection of elute was obtained by a photodiode array detector (DAD) which was set at 230 nm. ICH guidelines were adhered for validation of proposed method regarding specificity, sensitivity, precision, linearity, accuracy, system suitability and robustness. Calibration curves of BMZ and ABP were created in the range of 1–16 μg mL-1 with mean recovery percentage of 100.02 ± 1.245 and 99.74 ± 1.124, and detection limit of 0.20 μg mL-1 and 0.24 μg mL-1 respectively. BMZ stability was inspected under various ICH forced degradation conditions and it was found to be easily degraded in acidic and alkaline conditions. The results revealed the suitability of the described methodology for the quantitation of the impurity (ABP) in a BMZ pure sample. The determination of BMZ in pharmaceutical dosage forms was conducted with the described method and showed mean percentage recovery of 99.39 ± 1.401 and 98.72 ± 1.795 (n = 6), respectively. When comparing the described procedure to a reference HPLC method statistically, no significant differences between the two methods in regard to both accuracy and precision were found.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a stability indicating RP-HPLC-DAD method for the determination of bromazepam

Darwish

Ali²,

Naguib

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Bromazepam, like most of the benzodiazepine derivatives, is hydrolyzed in acidic aqueous solution leading to a series of degradation products [2]. The rate of acidic hydrolysis of bromazepam is believed to be dependent on the state of protonation of the pyridyl and azomethine nitrogen atoms [4,5]. It is hydrolyzed in acidic condition via intermediate product to give the final product of 2(2-amino-5-bromobenzoyl) pyridine (II) and glycine [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Different methods such as non-aqueous titration [8] flow injection [9], sequential injection [10], voltammetric [4,11], spectrophotometric [12,13], chromatographic [14 -16], capillary electrophoresis [17], and ion selective sensor methods [18] have been reported for the determination of bromazepam in pharmaceutical preparations, blood and plasma. Though BP recommends non-aqueous titration and spectrophotometric methods for the analysis of bromazepam in raw materials [8], there is no official method for its analysis in tablets.…”

Section: Introductionmentioning

confidence: 99%

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Takele

Attaya²,

Hymete³

et al. 2017

PHARMSCI

View full text Add to dashboard Cite

Introduction:Bromazepam is hydrolyzed in acidic aqueous solution leading to a series of degradation products. The rate of acidic hydrolysis is believed to be dependent on the state of protonation of the pyridyl and azomethine nitrogen atoms. Stability test is important in pharmaceutical industry to provide evidence on how the quality of an active substance or pharmaceutical product varies with time under the influence of a variety of environmental factors. Objective:The aim of the study was to develop a simple stability indicating method for the determination of bromazepam. Method:Bromazepam solution was prepared and forced degradation of bromazepam was performed under acid hydrolysis using sulphuric acid. High performance liquid chromatography determination of pure and degraded bromazepam and bromazepam-copper (II) complex was performed using reversed phase octyl C-8 column under isocratic conditions and the chromatographic conditions were set as follows; the flow rate of the mobile phase was 1.5 mL/min; injection volume was 10 μL, column temperature was 30 o C and the detector wavelength being 309 nm. Results:Bromazepam, its degradation product and bromazepam chelated with copper (II) were determined using the developed mobile phase with flow rate of 1.5 mL/min. Good separation with sharp peak, minimum tailing and retention time repeatability was obtained. The rate order, rate constant and half-life of degradation were also determined, and it was observed that the degradation reaction follows the first order kinetics. Conclusion:Chromatographic separation of bromazepam chelated with copper (II) was achieved and the method can be further used in drug manufacturing quality control.

show abstract

Oxidative behavior of aminohalogenbenzophenones – indirect determination of benzodiazepinooxazoles

et al. 1995

View full text Add to dashboard Cite

An electroanalytical study of three aminohalogenbenzophenones: 2-amino-5-chlorobenzophenone (MCB), 2-amino-2',5-dichlorobenzophenone (DCB), and 2-amino-5-bromo-2'-fluorobenzophenone (BrFB); metabolites of the psychotropic drugs benzodiazepinooxazoles and benzodiazepines, has been carried out at a glassy carbon electrode using different voltammetric techniques. The compounds studied are electrooxidizable in a hydroalcoholic medium (MeOH:H20, 10 : 90), 0.5 M KC1, and a pH range of 1-13. The oxidation processes are irreversible and predominantly diffusion controlled. On the basis of the oxidation process, voltammetric methods have been developed that allow the determination of aminohalogenbenzophenones from 0.2 to 15ppm for MCB and BrFB, and 0.2 to 14ppm for DCB; with a reproducibility in terms of relative standard deviation (RSD) lower than 5.2% for 10 determinations of lOppm solutions of MCB, DCB, and BrFB. Detection limits of 200ppb (pg/L) for MCB and DCB, and IOOppb for BrFB were obtained. The voltammetric method developed for DCB has been applied to the indirect determination of Cloxazolam in tablets.

show abstract

Determination of bromazepam and its urinary metabolites, with a previous hydrolysis reaction, by voltammetric and spectrophotometric techniques

Cited by 14 publications

References 0 publications

Development and validation of a stability indicating RP-HPLC-DAD method for the determination of bromazepam

Development and validation of a stability indicating RP-HPLC-DAD method for the determination of bromazepam

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Oxidative behavior of aminohalogenbenzophenones – indirect determination of benzodiazepinooxazoles

Contact Info

Product

Resources

About