Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry

Abstract

An efficient method for the simultaneous extraction and quantification of both jasmonic acid (JA) and methyl jasmonate (MeJA) from a single plant sample was developed. Plant tissues were firstly extracted by using 100% cold methanol, then the Sep-pak C 18 solid-phase extraction (SPE) cartridges were adopted for the purification of sample extract, and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry (LC-MS-MS) system. It was found that the accuracy was improved by using the extra standard for the estimation and correction of JA and MeJA losses in the extraction and purification process. The detection limits for JA and MeJA were 0.03 and 0.075 ng mL −1 , respectively, and the average recovery rate of JA and MeJA was 92.48% and 94.30%. The method was found to be reproducible and selective, yielding well isolated and easily detectable peaks for both JA and MeJA and simplifying the time-consuming extraction and purification. High-performance liquid chromatography (HPLC) system was employed as the control detection method for the LC-MS-MS system. The two systems were compared in their specificity and accuracy. jasmonic acid, methyl jasmonate, extraction, purification, LC-MS-MS Citation: Liu X, Yang Y L, Lin W H, et al. Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry.

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

“…The lamina joints of the cv ZH11 last leaf at 60, 65, 70, and 80 DAS (corresponding to stages 2, 4, 5, and 6) and the 0.5-cm adjacent part of the lamina joint of the last leaf blade at 60 and 65 DAS (corresponding to stages 2 and 4, as leaf samples) were collected for quantification assays of various hormones (Liu et al, 2010).…”

PgLOX6 encoding a lipoxygenase contributes to jasmonic acid biosynthesis and ginsenoside production in Panax ginseng

“…The extract was eluted from the cartridge with 0.3 ml of 100% methanol and collected for analysis. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurements were performed as described by Liu et al (2010). JA and MJ were purchased from Sigma-Aldrich.…”

Simple, Rapid, and Simultaneous Assay of Multiple Carboxyl Containing Phytohormones in Wounded Tomatoes by UPLC-MS/MS Using Single SPE Purification and Isotope Dilution

“…Targeted analysis of phytohormones can be carried out by LC-MS with multiple reaction monitoring (MRM) in a tandem mass spectrometer. Current LC-MS methods have mainly focused on detection of specific classes of phytohormones, such as auxin, 22,23 abscisic acid (ABA), [24][25][26] jasmonates, 27,28 salicylic acid (SA) and cytokinins. [29][30][31] Simultaneous determination of trace multiple phytohormones by LC-triple quadrupole mass spectrometry (MS/MS), which is a method that had been attracting increasing attention and which could provide detailed information for the understanding of molecular mechanisms of crosstalk, is still characterized by the time-consuming pretreatment and separation processes as well as co-eluted interferences of matrix.…”