Determination of blood indices of albino rats treated with aluminum chloride and investigation of antioxidant effects of vitamin E and C

Abstract

The current study aims to investigate hematological and biochemical blood indices of albino rats administrated aluminum chloride (AlCl 3 ) for eight weeks, and to study the therapeutic effects of vitamin E and C. AlCl 3 decreased the total red blood cell count (by 18%), hemoglobin (7%) and hematocrit (20%), and increased white blood cell count (67%), lymphocytes (29%), mean corpuscular volume (14%), mean corpuscular hemaglobin (6%) and platelets (33%). Administration of vitamin E with or without vitamin C failed to restore levels of red blood cell counts, hematocrit, mean corpuscular volume, mean corpuscular hemaglobin or platelets, but vitamin E on its own restored levels of white blood cells, hemaglobin and lymphocytes.AlCl 3 decreased serum glucose levels by 30%, and increased triglyceride (28%) and cholesterol (20%) levels; neither vitamin treatments restored the levels of these components. AlCl 3 increased levels of urea (12%), uric acid (77%) and creatinine (25%) compared to the controls, and vitamin E separately or together with vitamin C restored the levels of these nitrogen compounds.The activities of alanine aminotransferase, alkaline phosphatase, and aspartate aminotransferase were also increased by the AlCl 3 treatment; the first two but not aspartate aminotransferase were restored by vitamin E separately or together with vitamin C.We conclude that vitamin E separately or together with vitamin C suppressed cytogenetic injury and damage to some biochemical pathways of rat organs induced by AlCl 3 .

Possible Role of γ-Irradiated Ginseng in the Modulation of Some Biochemical Disorders Produced by Aluminum in Rats

“…AL toxicity may be mediated by free radical generation and alterations in antioxidant enzymes in vivo and in vitro (Tabaldi et al, 2009). The significant (P< 0.05) decreases in Hb, RBCs and Hct among AL-treated rats corroborate the findings of Abdel Aziz and Zabut (2011). The reduction in Hb content might be due to increased rate of destruction or reduction in the rate of formation of RBCs.…”

“…The activities of AST, ALT, ALP and LDH in serum were increased after toxicant administration. These findings are well correlated with those of Abdel Aziz and Zabut (2011) and Al-Hashem (2009). It is of interest to mention that in animals treated with toxicants, the serum levels of these enzymes elevated after liver damage, and thus alterations in liver function, because of increased membrane permeability or due to liver cell necrosis and cytosol leakage into the serum (Ozer et al, 2008).…”

<p>Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys</p>

“…62 The high levels of creatinine may reflect an abnormal glomerular filtration rate due to metals accumulation in kidneys. 63 Degeneration of renal tubular cells and mitochondrial damage due to NiNPs localization and accumulation, as revealed by this present work, may be related to these biochemical changes. 64 In this study, NiNPs caused damage to the mitochondria which also displayed a lower density.…”

Nephrotoxicity of nickel nanoparticles in rat: Effect of different doses

“…The high levels of creatinine could reflect the abnormal glomerular filtration rate due to the metals accumulation in kidney [28]. Among literatures, the results with respect to creatinine levels in response to NiNPs exposure were conflicting.…”