Determination of blood group antigens using electrophoresis of erythrocytes incubated with specific antibodies

Berest, Volodimir Petrovich; Borikov, Olexiy Yuriyovich; Kravchun, Pavlo Grigorievich; Leontieva, F. S.; Dielievska, Valentyna Yuriivna

doi:10.1002/sscp.202200017

Cited by 1 publication

(3 citation statements)

References 14 publications

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“…The obtained results are in accordance with previous results [3], that showed decrease of electrokinetic potential of erythrocytes under the influence of specific polyclonal antibodies and may testify to the association of increased ESR with decreased zeta potential of erythrocytes. The results obtained by the researchers show that ESR increases as the number of red blood cells changes, and room temperature affects the sedimentation rate -with an increase of the room temperature the ESR increases [10].…”

Section: Resultssupporting

confidence: 92%

“…The usefulness of this test has diminished as new methods for assessing inflammation activity have been developed. The test remains useful in the specific diagnosis of temporal arteritis, polymyalgia rheumatica, rheumatoid arthritis, Hodgkin's disease recurrence, vascular collagenosis or metastatic malignancy [2,3,9].…”

Section: Resultsmentioning

confidence: 99%

“…The erythrocyte sedimentation rate (ESR) remains a frequently used method and has a long history of monitoring inflammatory diseases [1][2][3]. Currently, the recommendations of the International Council for Standardization in Hematology and the National Committee for Clinical Laboratory Standards for measuring ESR are based on the Westergren method using samples with the anticoagulant EDTA.…”

Section: Introductionmentioning

confidence: 99%

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Group antibodies as factors specifically influencing erythrocytes sedimentation rate

Korzh,

Leontyeva,

Dunayeva

et al. 2023

OTP

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The use of the erythrocyte sedimentation rate (ESR) method has been recognized in monitoring the inflammation process, with a proven role of proinflammatory factors in the ESR increase. The aim. To reveal the role of group antibodies in the specific increase of ESR. Methods. For the study erythrocytes with EDTA anticoagulant were used. ESR was measured after the contact of erythrocytes with saline (negative control), standard anti-A and anti-B antibodies of the IgM class, polyclonal anti-A, anti- B, anti-A, B sera (experiment), as well as serum of group AB in a ratio 1 : 3: 0.2 ml of erythrocytes and 0.6 ml of saline or serum. The results were evaluated after one and 12 hours of incubation, at room temperature and at 4 °C. Results. Contact of anti-A IgM antibodies at a dilution of 1 : 30 with A erythrocytes led to an increase of ESR (from (3.25 ± 0.50) mm/h to (83.7 ± 1.60) mm/h) (p < 0.001) with a presence of a red precipitate. The contact of erythrocytes with polyclonal citrate plasma (or serum) in a ratio of 1 : 2 led to a similar increase in ESR in cases of the specific binding. Anti-A, B serum increased ESR of A erythrocytes up to (53.00 ± 2.64) mm/h (p < 0.001) with the presence of a red precipitate, while anti-B serum did not show such effect: (ESR — (5.25 ± 0.50) mm/h) (p > 0.05). It should be noted that the serum absorbed by the corresponding erythrocytes showed reduced ESR values. After the absorption of anti-A antibodies by A erythrocytes the serum lost the ability to specifically increase the ESR of A erythrocytes. Conclusions. Group antibodies are able to specifically promote ESR. The found ability can be reduced by the method of specific absorption of the serum. The physicians may consider the role of group specific autoimmune antibodies in developing high values of ESR. The therapy aimed to regulate the autoimmune humoral activation and specific absorption might be useful in normalization the ESR parameter.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%