Determination of biocytin

Ebrahim, H.; Dakshinamurti, K.

doi:10.1016/0003-2697(87)90398-8

Cited by 8 publications

(2 citation statements)

References 7 publications

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“…It was shown previously that the Km for biocytin of serum biotinidase was 10 /SM [21,26]. However, the serum concentration of biotin is of the order of 2 nM [24], and biocytin is hardly detectable in normal serum [22,27]. As shown in Fig.…”

Section: Effect Of Biotin Analogues On Biotin-binding Activitymentioning

confidence: 80%

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Role of human serum biotinidase as biotin-binding protein

Chauhan

Dakshinamurti

1988

Biochemical Journal

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Biotinidase shows two binding sites for biotin, with Kd = 59 and 3 nM respectively, and requires tryptophan and cysteine residues of the biotinidase protein for biotin-binding activity. Analysis of human serum by various column-chromatographic techniques indicates that biotinidase is the only protein which exchanges with labelled (+)-biotin. It was shown previously that epileptic patients receiving a high average dose of anticonvulsants (containing a carbamide group) have lower biotin concentrations than those receiving a low dose. We have shown in human serum and with purified biotinidase that these anticonvulsant drugs compete with biotin for binding to the protein moiety.

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Section: Effect Of Biotin Analogues On Biotin-binding Activitymentioning

confidence: 80%

“…Biotinidase activity was determined with N-[( + )biotinyl]-p-aminobenzoate and biocytin as substrates as previously described [21,22]. Sucrose-density-gradient analysis of human serum The procedure used for sucrose-density-gradient centrifugation was that of Martin & Ames [23].…”

Section: Biotinidase Activitymentioning

confidence: 99%

Role of human serum biotinidase as biotin-binding protein

Chauhan

Dakshinamurti

1988

Biochemical Journal

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Intestinal absorption of biotin and biocytin in the rat

1987

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The uptake of biotin and biocytin was investigated in rat intestine using the everted sac technique. It has been shown that at biotin and biocytin concentrations less than 40 and 50 nM respectively, absorption proceeds by a saturable process, whereas at higher concentrations uptake by passive diffusion predominates. Fractionation of solubilized brush border preparations indicates that biotinidase is the only protein which binds biotin in this preparation.

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