Determination of bioavailable phosphorus in water samples using bioassay methods

Dijkstra, Marcel L.; Auer, Martin T.; Kuczynski, Anika; Lambert, Renn Sterling

doi:10.1016/j.mex.2020.100807

Cited by 9 publications

(16 citation statements)

References 8 publications

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“…Furthermore, by quantifying BAP specifically, our assay adds insight regarding the measurement of the P form that is most relevant to eutrophication. Compared to other bio-assays for BAP, our assay can be run more quickly than those based on algae 7,8 , is easier to interpret compared to those based on bacteria, 18 and requires less sophisticated equipment than qPCR-based approaches. 17 Taken together, these attributes suggest that the yBAP assay could become a useful addition to the analytical toolbox used to address the eutrophication of freshwaters.…”

Section: Discussionmentioning

confidence: 99%

“…11 However, through this process, TP assays can quantify P in forms that are not typically bioavailable. As such, BAP is generally expected to fall between these two standard measurements, and through a combination of biological and chemical processes (e.g., enzyme secretion, local acidification), organisms access more P than is detected by SRP 8 . However, a portion of the total P typically remains unavailable to organisms, either because it resides as large particles or because the P is in a chemical form that is biologically inert 7 .…”

Section: Introductionmentioning

confidence: 99%

“…9,10 The quantity most relevant to the biological response to eutrophication is the bioavailable P (BAP), i.e., the portion of P accessible to organisms. 11,12…”

Section: Introductionmentioning

confidence: 99%

“…Other biological methods for detecting BAP include assays based on the growth of algae 9,11,24 or bacterioplankton. 25 While these approaches link BAP directly to the organisms most relevant to eutrophication, algal assays can take multiple weeks, use relatively large volumes of sample water, and require specialized growth environments.…”

Section: Introductionmentioning

confidence: 99%

“…25 While these approaches link BAP directly to the organisms most relevant to eutrophication, algal assays can take multiple weeks, use relatively large volumes of sample water, and require specialized growth environments. 11 Bacterioplankton-based methods can be run more rapidly, but are difficult to reproduce due to the uncharacterized nature of the cultures used thus far. 25 In summary, there is room for improved methodology in measuring BAP.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Development of a yeast-based assay for bioavailable phosphorus

Shepherd

Trentman

Tank

et al. 2021

Preprint

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Preventing eutrophication of inland freshwater ecosystems requires quantifying the phosphorous (P) content of the streams and rivers that feed them. Typical methods for measuring P assess soluble reactive P (SRP) or total P (TP) and require expensive analytical techniques that produce hazardous waste. Here we present a novel method for measuring the more relevant bioavailable P (BAP); this assay utilizes the growth of familiar baker's yeast, avoids production of hazardous waste, and reduces cost relative to measurements of SRP and TP. The yeast BAP (yBAP) assay takes advantage of the observation that yeast density at saturating growth increases linearly with provided P. We show that this relationship can be used to measure P in freshwater in concentration ranges relevant to eutrophication. In addition, we measured yBAP in water containing known amount of fertilizer and in samples from agricultural waterways. We observed that the majority of yBAP values were between those obtained from standard SRP and TP measurements, demonstrating that the assay is compatible with real-world settings. The cost-effective and nonhazardous nature of the yeast-based assay suggests that it could have utility in a range of settings, offering added insight to identify water systems at risk of eutrophication from excess phosphorus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…9,10 The quantity most relevant to the biological response to eutrophication is the bioavailable P (BAP), i.e., the portion of P accessible to organisms. 11,12…”

Section: Introductionmentioning

confidence: 99%