Determination of Bilirubin by Fiberoptic Biosensor

Li, X.; Fortuney, A.; Guilbault, G. G.; Suleiman, Aiman

doi:10.1080/00032719608000998

Cited by 19 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently an enzymic method based on the amperometric detection of hydrogen peroxide using immobilized bilirubin oxidase is described [7], however, according to the literature hydrogen peroxide is not produced in the course of the enzymic reaction [1,2,8]. A ®ber optic¯uorescence biosensor [9] and a multilayer enzyme electrode [10] have also been reported. Here we describe an enzymic method for the determination of bilirubin by using immobilized bilirubin oxidase in combination with a Clark-type oxygen electrode.…”

Section: Introductionmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

View full text Add to dashboard Cite

An enzymic method for the determination of bilirubin is described, based on the indirect electrochemical monitoring of bilirubin via its reaction with oxygen to biliverdin in the presence of bilirubin oxidase. Amperometric signals due to oxygen depletion were taken by means of an oxygen electrode incorporating an immobilized bilirubin oxidase membrane. The calibration plot shows linearity up to 2.0610 74 M. This concentration range corresponds to elevated bilirubin concentrations in adults and in newborn infants. The RSD of the method is 3.1 % (1.0610 74 M bilirubin, n 6). The method is interference free, fast (`3min) and easy to perform. The membrane retains almost 80 % of its initial activity after 30 successive measurements.

show abstract

Section: Introductionmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

View full text Add to dashboard Cite

show abstract

“…New applications and improvements are still being found for the method (15,16) and new fluorophores proposed (17). Much less frequently, methods based upon the measurement of O 2 consumption during the reaction by the quenching effect on a fluorophor have been proposed (18,19).…”

Section: Classical Enzymatic±fluorometric Methodsmentioning

confidence: 99%

Intrinsic fluorescence of enzymes and fluorescence of chemically modified enzymes for analytical purposes: a review

et al. 2001

View full text Add to dashboard Cite

In recent years our research group has developed new alternatives for fluorescence enzymatic determinations. First, we observed that the intrinsic fluorescence of enzymes changes during enzymatic reactions, proportionally to the substrate concentration, avoiding the combination of the enzymatic reaction with a fluorophore-involving reaction. The main disadvantage of this method is that the excitation and emission wavelengths of the enzymes are in the UV region of the spectrum. An alternative to overcome this problem consisted of covalently bonding the enzyme to a fluorophore. In this paper, an overview is given of all of the applications and future developments on both types of alternatives that we have developed. Apart from the analytical characteristics of the methods, we have also reviewed all of the information about mathematical models we have elaborated to date.

show abstract

“…The enzyme and the oxygen-sensitive dye were immobilized on an acrylamide polymer that was covalently attached to the tip of a silanized optical fiber by photopolymerization. Miniaturization of the sensor has enabled significant reduction of the response time (10 s) compared with other bilirubin sensors [47] and sub-microliter samples can be measured accurately. Adsorption of bilirubin and biliverdin on the sensor tip, however, causes passivation of the device, which affects both reproducibility and sensor lifetime.…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Fiber-optic biosensors – an overview

2002

View full text Add to dashboard Cite

This article reviews progress and developments during the past five years in the field of optical fiber biosensors. Because of the expense and time constraints associated with modern laboratory analysis, there is a growing need for real-time, low-cost technology that can be used industrially, environmentally, and clinically, and to monitor food processing. Miniaturization, integrated systems, and multianalyte determination have become key aspects of sensor development and efforts in this direction will also be discussed, with some pointers to likely directions of future research in the area. The review will provide information about the analytical characteristics and applications of fiber-optic biosensors classified depending on the biorecognition element employed - enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers.

show abstract

Determination of Bilirubin by Fiberoptic Biosensor

Cited by 19 publications

References 10 publications

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

Intrinsic fluorescence of enzymes and fluorescence of chemically modified enzymes for analytical purposes: a review

Fiber-optic biosensors – an overview

Contact Info

Product

Resources

About