Determination of bile acids in rat bile by high-performance liquid chromatography

Hayashi, Morimasa; Imai, Yumiko; Minami, Yuzo; Kawata, Satoshi; Matsuzawa, Yūji; Tarui, Seiichiro; Uchida, Keiji

doi:10.1016/0378-4347(85)80085-2

Cited by 17 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The qualitative composition of tridi-and monohydroxylated bile acids was generally similar to that reported by other authors (Hayashi, Imai, Minami, Kawata, Matsuzawa, Tarui & Uchida, 1985). The decrease in biliary cholesterol and phospholipid secretion, and in the lithogenic index, seen in rats with DSI resection fed diet A for 1 or 3 months contrasts with the findings of Bilhartz & Dietschy (1988), who reported that bile acid secretion paralleled bile lipid secretion.…”

Section: Discussionsupporting

confidence: 87%

Effect of intestinal resection, type of dietary fat and time on biliary lipid secretion in rats

Gómez-Ayala

Lisbona²,

López-Áliaga³

et al. 1994

Experimental Physiology

View full text Add to dashboard Cite

SUMMARYThe effects of time and the type of dietary fat on biliary physiology in rats with 50 % resection of the distal small intestine were investigated. The effects of ursodeoxycholic acid as an exogenous source of bile acid added to the diet were also studied. The fat composition of all diets was the same in quantitative terms (4 %), and differed only in the type of lipid supplied: olive oil (diet A) or one-third medium chain triglycerides, one-third sunflower seed oil and onethird olive oil (diet B). In resected rats given diet A for 1 or 3 months, there was a decrease in biliary secretion of cholesterol and phospholipids, and in the lithogenic index, with respect to the control group. Resected rats fed diet B for 1 or 3 months showed increases in biliary secretion of cholesterol and phospholipids, and in the lithogenic index, in comparison with resected rats fed diet A. The addition of ursodeoxycholic acid to diet B led to the decoupling of bile acid and bile lipid secretion.

show abstract

Section: Discussionsupporting

confidence: 87%

Effect of intestinal resection, type of dietary fat and time on biliary lipid secretion in rats

Gómez-Ayala

Lisbona²,

López-Áliaga³

et al. 1994

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…2; 22-to 26-min range). These peaks are most likely bile salts (Hayashi et al, 1985;Sakakura et al, 1993). This hypothesis is supported by the fact that these peaks can be removed by the addition of cholestyramine resin (a bile salt-sequestering agent) to the intestinal samples (data not shown).…”

Section: Gluten Digestion In Ratsmentioning

confidence: 77%

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

Gass¹,

Vora²,

Bethune³

et al. 2006

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Celiac Sprue is a multifactorial disease characterized by an intestinal inflammatory response to ingested gluten. Proteolytically resistant gluten peptides from wheat, rye, and barley persist in the intestinal lumen and elicit an immune response in genetically susceptible individuals. Here, we demonstrate the in vivo ability of a gluten-digesting protease ("glutenase") to accelerate the breakdown of a gluten-rich solid meal. The proenzyme form of endoprotease B, isoform 2 from Hordeum vulgare (EP-B2), was orally administered to adult rats with a solid meal containing 1 g of gluten. Gluten digestion in the stomach and small intestine was monitored as a function of enzyme dose and time by high-performance liquid chromatography and mass spectrometry. In the absence of supplementary EP-B2, gluten was solubilized and proteolyzed to a limited extent in the stomach and was hydrolyzed and assimilated mostly in the small intestine. In contrast, EP-B2 was remarkably effective at digesting gluten in the rat stomach in a dose-and time-dependent fashion. At a 1:25 EP-B2/gluten dose, the gastric concentration of the highly immunogenic 33-mer gliadin peptide was reduced by more than 50-fold within 90 min with no overt signs of toxicity. Evaluation of EP-B2 as an adjunct to diet control is therefore warranted in celiac patients.

show abstract

Ion‐pair high‐performance liquid chromatography of bile salt conjugates: Application to pig bile

Legrand-Defretin

Juste

Henry

et al. 1991

Lipids

View full text Add to dashboard Cite

The high-performance liquid chromatographic separation and quantitation of conjugated bile salts from pig bile is reported. Synthetic standards and bile samples were chromatographed on a C18 reversed phase column using acetonitrile/water/tetrabutyl ammonium phosphate as an isocratic mobile phase at a flow rate of 1 mL/min. Detection of the ion-pairs was at 214 nm. The method permits efficient separation of all conjugated pig biliary bile salts without prior modification or treatment of the samples. Analysis of 12 pig biles showed that 85% of the bile salts are conjugated to glycine. The three main conjugated bile salts were glyco-3 alpha,6 alpha,7 alpha-trihydroxy-5 beta-cholanoic acid (GHC), glyco-3 alpha,7 alpha-dihydroxy-5 beta-cholanoic acid (GCDC), and glyco-3 alpha,6 alpha-dihydroxy-5 beta-cholanoic acid (GHDC). Glyco-3 alpha-hydroxy-6-oxo-5 beta-cholanoic acid (G3 alpha 6oxo), tauro-3 alpha,7 alpha-dihydroxy-5 beta-cholanoic acid (TCDC), tauro-3 alpha,6 alpha,7 alpha-trihydroxy-5 beta-cholanoic acid (THC), and tauro-3 alpha,6 alpha-dihydroxy-5 beta-cholanoic acid (THDC) were found to contribute each for 4 to 5% to the total. An excellent correlation was found between the sum of conjugated bile salts quantitated by high-performance liquid chromatography (HPLC) and values obtained by conventional enzymatic assay. Simplicity, efficiency and relative rapidity of the method render it suitable for routine analyses.

show abstract

Determination of bile acids in rat bile by high-performance liquid chromatography

Cited by 17 publications

References 14 publications

Effect of intestinal resection, type of dietary fat and time on biliary lipid secretion in rats

Effect of intestinal resection, type of dietary fat and time on biliary lipid secretion in rats

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

Ion‐pair high‐performance liquid chromatography of bile salt conjugates: Application to pig bile

Contact Info

Product

Resources

About