Determination of bicyclol in dog plasma using liquid chromatography–tandem mass spectrometry

Li, Sheng; Hu, Jiye; Wang, Baolian; Chen, Hui; Li, Yan

doi:10.1016/j.jchromb.2010.06.014

Cited by 5 publications

(1 citation statement)

References 8 publications

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“…By now, the metabolomics approach using MIM has been successfully applied to discover biomarkers in plasma, rice, and so on [ 13 , 14 ]. In addition, the MIM has also been used to determine some compounds in biological samples, such as bicyclol and peptides, with acceptable accuracy, sensitivity, and selectivity [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Chemical Basis of Toxicity Reduction for Processed Roots of Stellera chamaejasme L. Using Ultraperformance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

Yang

Wang

et al. 2019

International Journal of Analytical Chemistry

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Some herbal medicines are treated with various processing methods to ensure their safe and effective use. However, chemical basis of toxicity reducing for most herbal medicines remains unclear. Stellera chamaejasme L., particularly the root (ruixianglangdu), is toxic. Thus, ruixianglangdu is commonly processed with vinegar or milk to reduce toxicity. Here, with help of multiple-ion monitoring (MIM)-based metabolomics, we comprehensively capture chemical information of ruixianglangdu. Then, 33 differential components between crude drugs and processed products were identified or tentatively characterized by multiple-ion monitoring–information dependent acquiring–enhanced product ion (MIM-IDA-EPI), whose level changed after being processed by vinegar or milk. It was found that flavonoids especially biflavonones could be the important chemical basis of toxicity reduction for processed ruixianglangdu. In addition, some coumarins and lignanoids could also play a role in reducing toxicity. It is believed that MIM-based metabolomics method was valuable for exploring chemical basis of toxicity reduction for processing. The data is critical to further study the mechanism of toxicity reducing for processed ruixianglangdu.

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Section: Introductionmentioning

confidence: 99%

Exploring Chemical Basis of Toxicity Reduction for Processed Roots of Stellera chamaejasme L. Using Ultraperformance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

Yang

Wang

et al. 2019

International Journal of Analytical Chemistry

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Pharmacokinetic analysis of plasma bicyclol by liquid chromatography–tandem mass spectrometry

Liu

Huang

Wen

et al. 2019

Biomedical Chromatography

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Bicyclol is a synthetic drug widely used to treat chronic hepatitis B. This study aimed to develop a selective, sensitive and high‐throughput liquid chromatography–tandem mass spectrometric method for the detection of bicyclol in human plasma. Bicyclol was detected using a multiple reaction monitoring mode, with ammonium adduct ions (m/z 408.2) as the precursor ion and the [M‐CH3]+ ion (m/z 373.1) subjected to demethylation as the product ion. Chromatographic separation was achieved using a Zobax Eclipse XDB‐C18 column with a gradient elution and a mobile phase of 2 mm ammonium formate and acetonitrile. Bicyclol was extracted from plasma matrix by precipitation. A linear detection response was obtained for bicyclol ranging from 0.500 to 240 ng/mL, and the lower limit of quantification was 0.500 ng/mL. The intra‐ and inter‐day precisions were all ≤7.4%, and the accuracies were within ±6.0%. The extraction recovery was >95.9%, and the matrix effects were between 96.0% and 108%. Bicyclol was found to be unstable in human plasma at room temperature, but the degradation was minimized by conducting sample collection and preparation in an ice bath. The validated method was successfully applied to investigate the pharmacokinetics of bicyclol tablets in six healthy Chinese volunteers.

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Application of physiologically based pharmacokinetic modeling in the prediction of pharmacokinetics of bicyclol controlled-release formulation in human

Wang

Liu

et al. 2015

European Journal of Pharmaceutical Sciences

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Determination of bicyclol in dog plasma using liquid chromatography–tandem mass spectrometry

Cited by 5 publications

References 8 publications

Exploring Chemical Basis of Toxicity Reduction for Processed Roots of Stellera chamaejasme L. Using Ultraperformance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

Exploring Chemical Basis of Toxicity Reduction for Processed Roots of Stellera chamaejasme L. Using Ultraperformance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

Pharmacokinetic analysis of plasma bicyclol by liquid chromatography–tandem mass spectrometry

Application of physiologically based pharmacokinetic modeling in the prediction of pharmacokinetics of bicyclol controlled-release formulation in human

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