Determination of benzimidazoles in pharmaceuticals and human serum by high-performance liquid chromatography

Sher, Nawab; Fatima, Nasreen; Perveen, Shahnaz; Siddiqui, Farhan Ahmed

doi:10.1080/10739149.2016.1184162

Cited by 14 publications

(10 citation statements)

References 21 publications

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“…The reported analytical methods include: spectrophotometry, 3-9 spectrouorimetry, 10-13 highperformance liquid chromatography (HPLC) [14][15][16][17] HPTLC, 18 thermogravimetry 19 and electrochemical methods.…”

Section: Introductionmentioning

confidence: 99%

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

Hamad

Ali

et al. 2018

RSC Adv.

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Two simple, sensitive, and rapid spectrofluorimetric methods were developed and validated for the determination of albendazole. The first method (method I) was based on the quenching effect of albendazole on the native fluorescence of erythrosine B. The fluorescence intensity was measured at 554 nm after extraction at 527 nm. In the second method (method II) the drug was reacted with lanthanum(III) ions to form a metal complex, which was measured at 340 nm after excitation at 295 nm.The suitable pH was 3.4 (Teorell-Stenhagen buffer) and pH 5.5 (phosphate buffer solution), for method I and II, respectively. The influence of experimental factors on the fluorescence intensity of the reaction products was investigated and optimized. The linear concentration ranges were 0.2-3.5 and 0.06-0.90 mg mL À1, with detection limits of 0.049 and 0.019 mg mL À1 for method I and II, respectively. ICH guidelines were followed for validation of the developed procedures, and the results were acceptable.The Gibb's free energy change of the reactions was À24.6 and À27.5 kJ mol À1 for method I and II, respectively. These negative values indicated the high feasibility of these reactions at ambient temperature. The proposed procedures were applied successfully for the determination of albendazole in commercial dosage forms and spiked human plasma. The results showed high precision, accuracy and recovery of the reported methods without any significant interference from pharmaceutical excipients or plasma components.

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Section: Introductionmentioning

confidence: 99%

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

Hamad

Ali

et al. 2018

RSC Adv.

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“…Nawab Sher et al (2016) utilized the HPLC method using a C18 analytical column as stationary phase. The mobile phase was 30:70 methanol: pH 2.5 phosphate buffer at a flow rate of 1.0 mL/min with absorbance detection at 235 nm.…”

Section: Discussionmentioning

confidence: 99%

“…However, the retention time of prednisolone was 10 min. The LOD, LOQ and coefficient of correlation (r 2 ) were 2.11, 6.39 ng/mL and 0.9995, respectively, for PSL in human serum (Sher et al, 2016). Jusko et al (1994) quantified prednisolone in human plasma by high-performance liquid chromatography (HPLC) with ultraviolet (UV) at 254 nm, where the detection limit was 10 ng/mL (Jusko et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Different techniques have been developed and employed for the measurement of prednisolone such as Radioimmunoassay (RIA) (Syedanaglbashl, Mizuchl, Yotsumoto, & Mlyachl, 1980), which requires expensive and sophisticated instruments, causes health hazard, requires regulatory approval, radioactive counting of large number of samples is time-consuming and a large amount of scintillation fluid is required for 3 H counting. The gas chromatography-mass spectroscopy (GC-MS) (Amendola, Garribbam, & Botre, 2003;Shibasakia et al, 2008), liquid chromatography-mass spectrometry (LC-MS) (van der Hoeven et al, 1997), liquid chromatography coupled to tandem mass spectrometry (LC/MS-MS) (Frerichs & Tornatore, 2004;Leporati et al, 2013;Vincenti et al, 2012), reversed-phase high-performance liquid chromatography (RP-HPLC) (Cho, Shin, & Yoo, 2003;Kurakula, Mohd, Samhuidrom, & Diwan, 2011), high-performance liquid chromatography (HPLC) (Chen et al, 2014;Doppenschmitt, Scheidel, Harrison, & Surmann, 1995;Jusko, Pyszczynski, Bushway, D'Ambrosio, & Mis, 1994;Sher, Fatima, Perveen, & Siddiqui, 2016), ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) (McWhinney, Briscoe, Ungerer, & Pretorius, 2010) and capillary separation of glucocorticoids coupled to various detectors are the standard methods used for the measurement (Rao, Petersen, Bissell, Okorodudu, & Mohammad, 1999). The limitation of GC-MS method is the requirement of derivatization of analyte before it is render volatile for detection (Amendola et al, 2003;Shibasakia et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Homologous ELISA for detection of prednisolone in human serum

Kumar

Singh

Shrivastav

2017

Food and Agricultural Immunology

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An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84-100.17%. The intraassay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93.

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“…Various analytical methods are developed for the determination of UA. These include electrochemical and chemiluminescence [7,8], high-performance liquid chromatography [9,10], and spectroscopic quantitative analysis [11,12]. As spectroscopic quantitative analysis only requires a small sample with easy preparation, the method for blood analysis attracts more attention [13,14].…”

Section: Introductionmentioning

confidence: 99%

Nonlinear Regression with High-Dimensional Space Mapping for Blood Component Spectral Quantitative Analysis

Cao

Zhang

Zhou

2018

Journal of Spectroscopy

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Accurate and fast determination of blood component concentration is very essential for the efficient diagnosis of patients. This paper proposes a nonlinear regression method with high-dimensional space mapping for blood component spectral quantitative analysis. Kernels are introduced to map the input data into high-dimensional space for nonlinear regression. As the most famous kernel, Gaussian kernel is usually adopted by researchers. More kernels need to be studied for each kernel describes its own high-dimensional feature space mapping which affects regression performance. In this paper, eight kernels are used to discuss the influence of different space mapping to the blood component spectral quantitative analysis. Each kernel and corresponding parameters are assessed to build the optimal regression model. The proposed method is conducted on a real blood spectral data obtained from the uric acid determination. Results verify that the prediction errors of proposed models are more precise than the ones obtained by linear models. Support vector regression (SVR) provides better performance than partial least square (PLS) when combined with kernels. The local kernels are recommended according to the blood spectral data features. SVR with inverse multiquadric kernel has the best predictive performance that can be used for blood component spectral quantitative analysis.

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Determination of benzimidazoles in pharmaceuticals and human serum by high-performance liquid chromatography

Cited by 14 publications

References 21 publications

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

Homologous ELISA for detection of prednisolone in human serum

Nonlinear Regression with High-Dimensional Space Mapping for Blood Component Spectral Quantitative Analysis

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