Determination of baseline Widal titre among apparently healthy population in Dehradun City

Kataria, Vijay

doi:10.9790/3008-0725355

Cited by 5 publications

(7 citation statements)

References 8 publications

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“…Our results were in concordance with those of the studies which were reported by some researchers in other endemic states of India. [24][25][26][27] For the anti-TH antibodies, an agglutinin titre of up-to 1:60 was discovered in the apparently healthy study population, whereas for the anti-TO antibodies, our result was lower (a titre of 1:40), which was in contrast to the reports of some other workers but it was in accordance with the reports of some previous studies. Several factors may have contributed to this discrepancy, because the differences in the antibody response may be due to the poorly standardized antigen preparation and the sharing of the antigen determinants with other Salmonellae.…”

Section: Discussionsupporting

confidence: 91%

Determination of Significant Baseline Widal Titre amongst Health Personnel & General Population of Ajmer City

Mohanpuria¹,

Sharma²,

Kamble³

et al. 2016

IJMRP

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Background: Enteric Fever is the most infectious disease on global scale and endemic in all parts of India. In a developing country like India laboratory diagnosis of enteric fever relies heavily on serological tests such as widal test. Knowledge of prevalent endemic titre in a region needs to be periodically updated to interpret the significance of the titre as to patient is indeed suffering from enteric fever or not. The present study has been undertaken to evaluate the baseline antibody titre for O, H antigens of Salmonella Enterica, serovar Typhi and AH, BH antigen of Salmonella Enterica, serovar Paratyphi among health personnel and general adult population of Ajmer (Rajasthan). Aim and objectives: To evaluate the significant endemic baseline titre of Ajmer city using quantitative Widal test to suggest the cut off value for interpreting a positive widal test. To compare the endemic baseline titre amongst the Health care providers and General Population. Material and methods: A total number of 600 healthy volunteers and 200 medical personnel working in Jawahar Lal Nehru Medical College, Ajmer were screened during period of study from 1 st April 2014 to 31 st December 2015. Non repetitive samples were collected from healthy adults aged 18 year to 70 year. Results and conclusions: Out of 600 screened samples for healthy volunteers 61% were males and 39% were females. The frequency of positive results was 43.67%. The agglutinins to S. Typhi were the most prevalent among the sera of various dilutions (33.16% for 'O' antigen and 43.33% for 'H' antigen) which was tested. The levels of agglutinins for Salmonella paratyphi AH and paratyphi BH were low (only 1.16% for AH and 1.83% for BH antigen respectively).Out of 200 samples of health personnel screened 69% were males and 31% were females. The frequency of positive results was 45.5%. The agglutinins to S. Typhi were the most prevalent among the sera of various dilutions (33.16% for 'O' antigen and 44.5% for 'H' antigen) which were tested. The levels of agglutinins for Salmonella paratyphi AH and paratyphi BH were low (only 4.0% for AH and 1.5% for BH antigen respectively). Based on the above results, it has been recommended that cutoff titre of 1:80 for anti-O antibodies and of 1:120 for the anti-H antibodies may be considered as diagnostic for enteric fever in Ajmer region of Rajasthan, India.

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Section: Discussionsupporting

confidence: 91%

Determination of Significant Baseline Widal Titre amongst Health Personnel & General Population of Ajmer City

Mohanpuria¹,

Sharma²,

Kamble³

et al. 2016

IJMRP

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“…12 Kataria et al reported a titre of >1:160 in 4% of healthy population for O antigen and 3.6% against H-antigen from Dehradun. 7 In contrast to these studies some studies from India, Prashant 13 and Punia 14 have shown TO titre of >1:160 in none of the 490 and 255 normal subjects tested and TH titres of >1: 160 only in 0.6% (3 out of 490) and 1.5% (4 out of 255), respectively.…”

Section: Discussionmentioning

confidence: 88%

“…The baseline titres in turn differ substantially across different regions depending on the endemicity of typhoid in each area, which has been changing over time. 7 Hence, establishing or updating of the baseline Widal titre for a particular area is an important factor in the proper interpretation of the Widal test. [8][9][10] The aim of the present study is to establish the baseline Widal titre among the healthy population of Wayanad district, Kerala.…”

Section: Introductionmentioning

confidence: 99%

Study of baseline Widal titres in a healthy adult population of Wayanad district, Kerala, India

et al. 2014

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The Widal test is still widely used in diagnosing typhoid fever in developing countries. Often, specific chemotherapy is administered based on a single Widal test. Interpretation of a single Widal test, in turn, depends on the baseline agglutinin titres among the local population in that area. Hence, we aimed to find out the baseline titres in our area. A single blood sample was collected from hospital outpatients with non-infectious diseases, the patients being aged above 18 years and residing in Wayanad district continuously for at least 5 years. The test was performed according to the Widal test kit manufacturer's instructions and interpreted using standard guidelines. The baseline titre for Anti TO, TH, AH and BH agglutinins in our area was found to be 1:40, 1:80, 1:40 and 1:40, respectively.

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“…Baseline Widal titer study done in rural Pondicherry recommended ≥160 for O and H agglutinins while for AH to be ≥80 and BH to be ≥40 to be considered a significant titer in diagnosing typhoid [14]. A study done in Dehradun on apparently healthy population found agglutination titer for TO ≥20 in 28%, TO ≥40 in 24.6%, TO ≥80 in 10.3%, TH ≥80 in 7.6%, AH ≥20 in 6.6%, and BH ≥20 in 4.6% [15].…”

Section: Discussionmentioning

confidence: 99%

Use of Widal Test in Diagnosis of Typhoid Fever in North Indian Population by Estimating Baseline Titer in Control Group

Shetty

2017

Asian J Pharm Clin Res

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Objective: This study was undertaken to establish a cutoff significant titer for Widal test using healthy volunteers as control group. Utilizing the baseline titer obtained from the control group, a diagnosis of typhoid fever was made in the test group comprising outpatients and inpatients. Methods: Blood samples were collected from healthy volunteers and patients attending G.S Medical College and Hospital, Pilkhuwa, over a period of 6 months from September 2016 to March 2017. Antibodies to Salmonella typhi (TO, TH) and Paratyphi A (AH) and Paratyphi B (BH) are determined by this tube agglutination test. A total of 124 healthy controls and 303 patients having clinical suspicion of typhoid fever were subjected to Widal test. Results: In healthy control group, titer TO ≥20 was observed in 43 (34.7%), TO ≥40 in 48 (38.7%), TO≥80 in 25 (20.2%), and titer TO ≥160, TO ≥320 was observed in none of the control group. Titer TH ≥40 in 58 (46.8%), AH ≥40 in 7 (5.6%), and BH ≥40 in 13 (10.5%) were observed in the control group. Among the test group, 96 (31.7%) sera were positive out of 303 clinically suspected enteric fever by the Widal test. Among different age group studied, 34 (46.6%) patients belonged to the age group of 11-20 years which formed the highest followed by the age group of 21-30 years (33.3%). Conclusion: Based on the study, a cutoff titer of ≥160 for anti-O and anti-H antibodies and titer of ≥80 for anti-AH and anti-BH antibodies be considered as significant titer in diagnosis of enteric fever in this region. The baseline titer helps in early recognition and treatment of this serious health problem.

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Determination of baseline Widal titre among apparently healthy population in Dehradun City

Cited by 5 publications

References 8 publications

Determination of Significant Baseline Widal Titre amongst Health Personnel & General Population of Ajmer City

Determination of Significant Baseline Widal Titre amongst Health Personnel & General Population of Ajmer City

Study of baseline Widal titres in a healthy adult population of Wayanad district, Kerala, India

Use of Widal Test in Diagnosis of Typhoid Fever in North Indian Population by Estimating Baseline Titer in Control Group

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