Determination of Atrazine in Vegetable Samples Using a Dipstick Immunoassay Format

Gabaldón, José Antonio; Maquieira, Ángel; Puchades, Rosa

doi:10.1080/713714611

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…A clear decrease in maximum signal and sensitivity was observed. Similar results of the influence of organic solvent on the performance of ELISA have been reported in the literature [23,24]. Therefore, 2% of solvent, which corresponds to 1:25 dilution (1:50 in the well) of extract, was the maximum solvent concentration tolerated by this ELISA.…”

Section: Study Of the Extraction Processsupporting

confidence: 81%

Rapid immunoanalytical method for the determination of atrazine residues in olive oil

Garcés-García

Morais

Martinez³

et al. 2004

Analytical and Bioanalytical Chemistry

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A sensitive, simple and reliable method has been developed for the determination of atrazine in extra virgin olive oil. The analytical procedure involves direct extraction of the target analyte from oil matrix with methanol and a freezing clean-up step (-80 degrees C) followed by plate or sensor immunoassay determination. A detection limit of 0.7 ng/mL, with a dynamic range from 1.0 to 10.4 ng/mL, was reached. The method was highly selective for atrazine and propazine, showing little or no cross-reactivity to other similar compounds. The excellent recoveries obtained (mean value 91.3%) confirm the potential of this approach to detect atrazine in olive oil for application as screening and complementary method in pesticide regulatory and food safety programs. The proposed method correlates well with the reference gas chromatography (GC-MS) technique.

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Section: Study Of the Extraction Processsupporting

confidence: 81%

Rapid immunoanalytical method for the determination of atrazine residues in olive oil

Garcés-García

Morais

Martinez³

et al. 2004

Analytical and Bioanalytical Chemistry

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“…Dipstick immunoassays usually follow the standard ELISA procedure but use a membrane as antibody-coating support and rely on color development as seen with the naked eye. Their use as a diagnostic tool for detecting pesticides is becoming more common ( − ).…”

Section: Introductionmentioning

confidence: 99%

Development of a Microtiter Plate ELISA and a Dipstick ELISA for the Determination of the Organophosphorus Insecticide Fenthion

Cho

Kim

Hammock

et al. 2003

J. Agric. Food Chem.

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In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA showed an IC(50) value of 1.2 microg/L with a detection limit of 0.1 microg/L. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides. The use of the dipstick format using Immunodyne as a support membrane allowed the quick visual detection of fenthion in concentrations >10 microg/L. The IC(50) value of the dipstick format using reflectance detection was 15 microg/L with a detection limit of 0.5 microg/L. The recoveries of fenthion from spiked vegetable samples using the two formats without any prior enrichment or cleanup steps were 87-116%.

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“…Immunoassays can meet these requirements. Numerous related methods for the detection of atrazine have been reported, such as enzyme-linked immunosorbent assay (ELISA) [10][11][12][13][14][15], colloidal gold-based immunochromatographic (ICG) strip assay [16][17][18], time-resolved fluorescent immunoassay (TRFIA) [19] and liposome immune lysis assay (LILA) [20]. The specific features of each study are listed and compared in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

Sheng

Yuan

et al. 2012

Microchim Acta

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We have developed a heterologous direct competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic (ICG) strip for the determination of the herbicide atrazine in water samples. The ELISA had a half-maximum inhibition concentration (IC 50 ) of 0.12 ng mL −1 and a limit of detection (LOD, calculated as the IC 15 value) of 0.01 ng mL −1 . The average of recoveries for all spiked water samples was 96.5%. There was a good correlation between the data determined by this ELISA and those obtained by high performance liquid chromatography (HPLC) (r 2 00.996). The visual LOD of the ICG strip assay was 2 ng mL −1 . The assay process only took 10 min, and no sample pretreatment was required. Its high specificity, sensitivity and fast detection made the strip well suited for on-site screening of atrazine in water samples. Both the ELISA and the ICG strip assay are useful for rapid analysis of a large number of water samples at low cost.

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Determination of Atrazine in Vegetable Samples Using a Dipstick Immunoassay Format

Cited by 9 publications

References 10 publications

Rapid immunoanalytical method for the determination of atrazine residues in olive oil

Rapid immunoanalytical method for the determination of atrazine residues in olive oil

Development of a Microtiter Plate ELISA and a Dipstick ELISA for the Determination of the Organophosphorus Insecticide Fenthion

Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

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