Determination of ascorbic and dehydroascorbic acid in potatoes (Solanum tuberosum) and straberries using ion-exclusion chromatography

Graham, W. Donald; Annette, Donald

doi:10.1016/0021-9673(92)80329-s

Cited by 51 publications

(20 citation statements)

References 7 publications

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“…Several authors have suggested the use of MPA for optimal extraction and preservation of L-AA [25][26][27]. Similarly, in the current study MPA was found to extract and stabilize L-AA with acceptable accuracy and precision.…”

Section: Reagent Standards and Mobile Phase Stabilitysupporting

confidence: 74%

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Parbhunath¹

2014

J Anal Bioanal Tech

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In accordance with national and international regulatory standards, namely ISO/IEC 17025, the validation of chromatography methods is becoming necessary. This study provides an optimized and fully validated reversephase high performance liquid chromatography (RP-HPLC) assay with ultra-violet (UV) detection for the measurement of L-ascorbic acid (L-AA) in fruit, vegetable and food products.Several commercial fruit juices and teas, fresh fruit and vegetables and food extract products were analyzed using a high performance liquid chromatographic system with UV detection. Chromatographic separation of L-AA was achieved on a reverse phase C 18 150 mm×4.6 mm, 0.5 µm column with UV detection of 245 nm at room temperature. Distilled water/acetonitrile/formic acid (99: 0.9: 0.1, v/v/v) at a flow rate of 1 mLmin -1 was used as the mobile phase, in isocratic mode. Samples were extracted in 4.5% metaphosphoric acid solution and filtered through a 0.45 µm membrane. The method was validated for accuracy, precision, linearity, range, limit of detection, limit of quantification, specificity, stability, robustness and system suitability in accordance with ISO 17025 validation requirements. Validation results demonstrated a linear response within a range of 5 to 125 µg/mL with a correlation coefficient of 0.999 was obtained. Mean recoveries ranged from 99 to 103% and 92 to 96% for L-AA standards and samples, respectively. The method was found to be precise (COV's <5%) and specific with no interferences from coexisting peaks. The LOD and LOQ were 0.61 µg/mL and 1.84 µg/mL respectively.The successful optimization and validation of the proposed method should make it easily applicable for routine laboratory analysis of L-AA measurement in various fruit and vegetable products.

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Section: Reagent Standards and Mobile Phase Stabilitysupporting

confidence: 74%

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Parbhunath¹

2014

J Anal Bioanal Tech

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“…The assay was based on the methods developed by Graham and Annette (1992). A stock solution of ascorbic acid (AA) (200 µg/ml) was prepared by dissolving 20 mg AA (BDH, Poole, UK) in 100 ml 62.5 mM metaphosphoric acid (BDH, Poole, UK).…”

Section: Ascorbic Acid Assaymentioning

confidence: 99%

Defining the stability interfaces of apple juice: Implications on the optimisation and design of High Hydrostatic Pressure treatment

Valdramidis

Graham

Beattie

et al. 2009

Innovative Food Science & Emerging Technologies

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“…Endogenous ascorbic acid was extracted and analyzed by the method of Grahm and Annette (1992) with a slight modification (Kwon et al 2001). Cucumber leaves were homogenized on ice with a mortar and a pestle in 62.5 mM metaphosphoric acid (1:2, w/v).…”

Section: Determination Of H 2 O 2 and Ascorbate Contentsmentioning

confidence: 99%

Chloroplast Cu/Zn-superoxide dismutase is a highly sensitive site in cucumber leaves chilled in the light

Choi

Jeong

et al. 2002

Planta

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Light-chilling stress, the combination of low-light illumination and low temperature, preferentially inactivated photosystem I (PSI) of cucumber (Cucumis sativus L.) leaves, resulting in the photoinhibition of photosynthesis. The extent of PSI photoinhibition, determined in vivo by monitoring absorption changes around 810 nm (induced by far-red light), was closely correlated with the redox state of the PSII electron acceptor Q(A), measured as the chlorophyll fluorescence parameter, 1-qP, where qP is a photochemical quenching coefficient. In contrast, the decrease in the far-red-induced leaf absorptance signal was not well correlated with the limited fragmentation of the PsaA/B gene products in the PSI reaction center after the light-chilling stress. Amongst various enzymes involved in the photooxidative damage such as superoxide dismutase (SOD), ascorbate peroxidase, and NAD(P)H dehydrogenase, only SOD was inhibited by light-chilling treatment. Further, an approximately 3-fold increase in the leaf content of H(2)O(2), a potent inhibitor of Cu/Zn-SOD, was observed after light-chilling stress. From these results, we suggest that Cu/Zn-SOD is the primary target of the light-chilling stress, followed by subsequent inactivation of PSI by reactive oxygen species.

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Determination of ascorbic and dehydroascorbic acid in potatoes (Solanum tuberosum) and straberries using ion-exclusion chromatography

Cited by 51 publications

References 7 publications

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products

Defining the stability interfaces of apple juice: Implications on the optimisation and design of High Hydrostatic Pressure treatment

Chloroplast Cu/Zn-superoxide dismutase is a highly sensitive site in cucumber leaves chilled in the light

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