Determination of Ascorbic Acid in Plasma and Urine by High Performance Liquid Chromatography with Ultraviolet Detection

Abstract

A reliable simple reversed-phase liquid chromatographic method for the routine determination of ascorbic acid in plasma and urine with ultraviolet detection is described. This method enables the complete separation of the ascorbic acid peak from others with a recovery of above 95% within 8 minutes. The method can be used for analysing multiple samples within a day. In addition, the storage conditions and stability of ascorbic acid in plasma and urine were investigated. Samples of plasma and urine can be stored on ice in darkness for at least 60 min without reduction of ascorbic acid concentration. Prepared samples can be stored in darkness at 4 degrees C for at least 120 min and in liquid nitrogen for 42 days.

Metabolic Clearance of the Antioxidant Ascorbic Acid in Surgical Patients1

“…The urine volume was measured for the calculation of ascorbic acid and creatinine clearance. The method used to analyze ascorbic acid in plasma and urine was previously published [10]. All specimens were collected in tubes containing 1.6 mg/ml ethylenediaminetetracetic acid (EDTA) as anticoagulant.…”

Early postoperative substitution procedure of the antioxidant ascorbic acid

“…For analysing AA in plasma, the previously published method was used [9]. In brief, all specimens were collected in tubes with 1.6 mg/ml ethylenediaminetetraacetic acid as anticoagulant.…”

The Effect of Intravenous Iron on Oxidative Stress in Hemodialysis Patients at Various Levels of Vitamin C

“…For HPLC analysis, serum with metaphosphoric acid was mixed with acetonitrile (1: 2), centrifuged at 2,400 g for 5 min, and the supernatant was transferred into a dark vial. Vitamin C was measured by HPLC according to Margolis et al [11] and Margolis and Duewer [12] using UV detection described by Rumelin and Fauth [13] . For chromatographic separation, an LC-NH2 column (250 !…”