Determination of artemisitene in rat plasma by ultra‐performance liquid chromatography/tandem mass spectrometry and its application in pharmacokinetics

Abstract

The UPLC/MS/MS assay was validated for linearity, accuracy, stability, extraction recovery, matrix effects, and intra-day and inter-day precision. The method, for the first time, achieved some pharmacokinetic parameters and was successfully applied to a pharmacokinetic study Copyright © 2017 John Wiley & Sons, Ltd.

Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLC/Q-TOF-MS

“…e same pattern was with peak 60 weighed 723.2134 (C 33 H 40 O 18 ) and therefore it was suspected to be Jasmigeniposide A [22]. Peaks us, artemisitene was detected at peak of 51, and peak 48 was identified as artemisitene [38].…”

Quantitative determination of proxalutamide in rat plasma and tissues using liquid chromatography/tandem mass spectrometry

“…The established methods were validated according to standard guidelines used for method validation in many published studies for confirming the specificity, linearity, accuracy and precision, recovery, and matrix effect in bioanalytical methods 18–22 …”

“…The established methods were validated according to standard guidelines used for method validation in many published studies for confirming the specificity, linearity, accuracy and precision, recovery, and matrix effect in bioanalytical methods. [18][19][20][21][22] The intra-day precision (n = 5 for each QC sample, each individually prepared and analyzed) was evaluated for rat plasma on the same day, and the inter-day precision was evaluated on three consecutive days.…”

Investigation of metabolic profile of pimavanserin in rats by ultrahigh‐performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry

“…Establishing a high‐throughout, high‐specificity and high‐sensitivity analytical method is necessary . Among the available analytical techniques, ultrahigh‐performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC/FT‐ICR‐MS) is a powerful and reliable analytical approach for detecting and identifying drug metabolites in vivo .…”

“…[13][14][15] Establishing a high-throughout, high-specificity and high-sensitivity analytical method is necessary. 16,17 Among the available analytical techniques, ultrahigh-performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC/FT-ICR-MS) is a powerful and reliable analytical approach for detecting and identifying drug metabolites in vivo. [18][19][20] It can provide not only high mass accuracy/resolution and elemental composition, but also fragmentation information, which can facilitate the structural elucidation of metabolites.…”