Determination of artemisinin and its analogs in Artemisia annua extracts by capillary electrophoresis – Mass spectrometry

Nagy, Cynthia; Pesti, Anna; Andrási, Melinda; Vasas, Gábor; Gáspár, Attila

doi:10.1016/j.jpba.2021.114131

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…The methanol and chloroform extracts obtained from the roots and aerial parts of the five Artemisia species were subsequently subjected to an in-depth LC-HRMS/MS analysis. The assignment of the peaks observed in the base peak chromatograms (BPCs) of the extract samples was performed by comparing the spectrometric data with the relevant literature [54][55][56][57][58][59][60][61] or online databases (KNApSacK; METLIN; NIST Chemistry WebBook). The metabolite profiling allowed the annotation of 73 compounds belonging to different phytochemical classes, such as phenolic acids, flavonoids, sesquiterpenes, organic acids, sugars, coumarins, triterpenes, lignans and fatty acids (Table 2, Table S1).…”

Section: Lc-hrms/ms Analysismentioning

confidence: 99%

Unveiling the Phytochemical Profile and Biological Potential of Five Artemisia Species

et al. 2022

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The Artemisia L. genus comprises over 500 species with important medicinal and economic attributes. Our study aimed at providing a comprehensive metabolite profiling and bioactivity assessment of five Artemisia species collected from northeastern Romania (A. absinthium L., A. annua L., A. austriaca Jacq., A. pontica L. and A. vulgaris L.). Liquid chromatography–tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of methanol and chloroform extracts obtained from the roots and aerial parts of the plants led to the identification of 15 phenolic acids (mostly hydroxycinnamic acid derivatives), 26 flavonoids (poly-hydroxylated/poly-methoxylated flavone derivatives, present only in the aerial parts), 14 sesquiterpene lactones, 3 coumarins, 1 lignan and 7 fatty acids. Clustered image map (CIM) analysis of the phytochemical profiles revealed that A. annua was similar to A. absinthium and that A. pontica was similar to A. austriaca, whereas A. vulgaris represented a cluster of its own. Correlated with their total phenolic contents, the methanol extracts from both parts of the plants showed the highest antioxidant effects, as assessed by the DPPH and ABTS radical scavenging, CUPRAC, FRAP and total antioxidant capacity methods. Artemisia extracts proved to be promising sources of enzyme inhibitory agents, with the methanol aerial part extracts being the most active samples against acetylcholinesterase and glucosidase. All Artemisia samples displayed good antibacterial effects against Mycobacterium tuberculosis H37Ra, with MIC values of 64–256 mg/L. In conclusion, the investigated Artemisia species proved to be rich sources of bioactives endowed with antioxidant, enzyme inhibitory and anti-mycobacterial properties.

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Section: Lc-hrms/ms Analysismentioning

confidence: 99%

Unveiling the Phytochemical Profile and Biological Potential of Five Artemisia Species

et al. 2022

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“…Since these analytes were crucial to the analysis and comparisons between optical trains, it was essential to eliminate the concern of contamination from the ESI background and from the double-sided tape utilized as a mount. Full MS and MS/MS scans were collected, where the [M + H + ] + , [M – H 2 O + H + ] + , and other previously reported ions , (data not shown) were monitored and selected as precursors for fragmentation. When comparing MS/MS spectra from previous work with A. annua , , the fragmentation patterns did not align, ultimately suggesting the fragmentation of possible background ions.…”

Section: Resultsmentioning

confidence: 99%

“…Full MS and MS/MS scans were collected, where the [M + H + ] + , [M – H 2 O + H + ] + , and other previously reported ions , (data not shown) were monitored and selected as precursors for fragmentation. When comparing MS/MS spectra from previous work with A. annua , , the fragmentation patterns did not align, ultimately suggesting the fragmentation of possible background ions. These ions were an order of magnitude lower in the full MS spectra of the double-sided tape compared to the leaf.…”

Section: Resultsmentioning

confidence: 99%

Incorporation of Three Different Optical Trains into the IR-MALDESI Mass Spectrometry Imaging Platform to Characterize Artemisia annua

Ashbacher,

Mills,

Sohn

et al. 2024

J. Am. Soc. Mass Spectrom.

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Artemisinin is the leading medication for the treatment of malaria and is only produced naturally in Artemisia annua. The localization of artemisinin in both the glandular and non-glandular trichomes of the plant makes it an ideal candidate for mass spectrometry imaging (MSI) as a model system for method development. Infrared matrix-assisted laser desorption electrospray ionization MSI (IR-MALDESI-MSI) has the capability to detect hundreds to thousands of analytes simultaneously, providing abundance information in conjunction with species localization throughout a sample. The development of several new optical trains and their application to the IR-MALDESI-MSI platform has improved data quality in previous proof-of-concept experiments but has not yet been applied to analysis of native biological samples, especially the MSI analysis of plants. This study aimed to develop a workflow and optimize MSI parameters, specifically the laser optical train, for the analysis of Artemisia annua with the NextGen IR-MALDESI platform coupled to an Orbitrap Exploris 240 mass spectrometer. Two laser optics were compared to the conventional set up, of which include a Schwarzschild-like reflective objective and a diffractive optical element (DOE). These optics, respectively, enhance the spatial resolution of imaging experiments or create a square spot shape for top-hat imaging. Ultimately, we incorporated and characterized three different optical trains into our analysis of Artemisia annua to study metabolites in the artemisinin pathway. These improvements in our workflow, resulted in high spatial resolution and improved ion abundance from previous work, which will allow us to address many different questions in plant biology beyond this model system.

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Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

Wang

Gong

Liu

et al. 2022

J of Separation Science

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So far, the potential of capillary electrophoresis in the application fields has been increasingly excavated due to the advantages of simple operation, short analysis time, high‐resolution, less sample consumption, and low cost. This review examines the implementations and advancements of capillary electrophoresis in different application fields (environmental, pharmaceutical, clinical, and food analysis) covering the literature from 2019 to 2021. In addition, ultrasmall sample injection volume (nanoliter range) and short optical path lead to relatively low concentration sensitivity of the most frequently used ultraviolet‐absorption spectrophotometric detection, so the pretreatment technology being developed has been gradually utilized to overcome this problem. Despite the review being focused on the development of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis, the new sample pretreatment techniques of microextraction and enrichment fit excellently to capillary electrophoresis in recent three years are also described briefly.

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Determination of artemisinin and its analogs in Artemisia annua extracts by capillary electrophoresis – Mass spectrometry

Cited by 9 publications

References 27 publications

Unveiling the Phytochemical Profile and Biological Potential of Five Artemisia Species

Unveiling the Phytochemical Profile and Biological Potential of Five Artemisia Species

Incorporation of Three Different Optical Trains into the IR-MALDESI Mass Spectrometry Imaging Platform to Characterize Artemisia annua

Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

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