Determination of artemether and dihydroartemisinin in blood plasma by high-performance liquid chromatography for application in clinical pharmacological studies

A liquid chromatographic-tandem mass spectrometric method for determination of artesunate and its metabolite dihydroartemisinin in human plasma

“…The autosampler was kept at a temperature of 4 • C to minimize stability problems [27]. It was necessary to leave the samples in the autosampler for 15 h (data not shown) to enable full ␣/␤ DHA epimer equilibration which is in agreement with previous reports [14,28]. The column oven was kept at 40 • C to reduce back pressure and to increase peak efficiency and reduce separation times.…”

On-column epimerization of dihydroartemisinin: An effective analytical approach to overcome the shortcomings of the International Pharmacopoeia monograph☆

“…Numerous HPLC methods were developed for the analysis and plasma levels monitoring of 2, formerly based on two main detection strategies: reductive electrochemical (EC) [11][12][13][14][15][16][17] and UV detection [18][19][20] involving pre-or post-column derivatization. The latter approach lacks specificity in that metabolites of the drug are also converted, in many instances, to identical UV-absorbing products.…”

A clinical and pharmacokinetic trial of six doses of artemether-lumefantrine for multidrug-resistant Plasmodium falciparum malaria in Thailand.

“…V. Navaratnam (University Sains Malaysia, Penang, Malaysia) as previously described. 27,28 The range of intra-assay coefficients of variation for the quality control (validation) samples were 9.1-10.3% for artemether, 8.4-9.1% for DHA, and 6.4-9.8% for lumefantrine. The limits of measurement for the three compounds were 2.5, 1.25, and 5 ng/ml, respectively, and concentrations below these limits were taken as zero.…”