Determination of arsenic species by high-performance liquid chromatography-inductively coupled plasma mass spectrometry

Abstract

Arsenic(lll), As", monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA) and arsenobetaine (AsB) were separated by high-performance liquid chromatography (HPLC) and determined on-line by inductively coupled plasma mass spectrometry (ICP-MS). Two forms of HPLC were used: ion pairing and ion exchange, with absolute detection limits for arsenic ranging between 50 and 300 pg. These detection limits were independent of the arsenic species when peak area was used for quantification. Anion pairing was found generally to be more sensitive to changes in the matrix of the sample injected. Anion exchange was more tolerant because of the higher buffering capacity of the mobile phase. Cation pairing was found suitable for the determination of DMAA and AsB in a biological sample containing high concentrations of salts.

Speciation of five arsenic species (arsenite, arsenate, MMAAV, DMAAV and AsBet) in different kind of water by HPLC-ICP-MS

“…This gradient allowed a correct separation of the five molecules within 30 min including column re-equilibration time. In order to improve the ionisation of arsenic in the plasma, 3% (v/v) of methanol have been added in the mobile phase (Beauchemin et al, 1989;Larsen and Stü rup, 1994). In spite of the fact that Brisbin et al (2002) claimed that the use of carbonate as mobile phase brings less deposit than phosphate, no signal decrease was observed with ten repetitions.…”

Measurement of elemental speciation by liquid chromatography -- inductively coupled plasma mass spectrometry (LC-ICP-MS) with the direct injection nebulizer (DIN)

“…Plugging was not a serious problem during the chromatography work; eluents containing metal salts were avoided because they also suppressed analyte signals by the usual matrix effects (20)(21)(22)(23)(24)(25)(26).…”

“…Background, » 200 counts s"' HPLC conditions as in Table 3. Background, » 400 counts s"' of the most attractive detection systems for elemental speciation because of its excellent detection limits, its multielement capability, and its ability to measure isotope ratios (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28).…”

“…So far, various separation schemes, including reversed-phase (RP) (16)(17)(18)(19)(20), reversed-phase ion-pairing (RP-IP) (21)(22)(23)(24)(25)(26)(27)(28)(29), ion chromatography (IC) (16,23,25,(30)(31)(32)(33)(34)(35), size exclusion chromatography (SEC) (16,(36)(37)(38)(39)(40)(41)(42)(43), supercritical fluid chromatography (SPC) (44) and gas chromatography (GC) (45,46) have been coupled with ICP-MS. Nevertheless, there is still substantial room for improvement including better chromatographic separations and lower detection…”

High-Performance Liquid Chromatographic Determination of Iron-Containing Proteins with On-Line Inductively Coupled Plasma Mass Spectrometric Detection

“…Thus it is a logical consequence to use ICP/ MS as an element-specific detector, in place of non-specific refractive index or ultraviolet detectors for HPLC. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] For biological macromolecules, gel permeation chromatographic (GPC) columns are generally used to separate the compounds according to the size.…”