Determination of arsenic compounds using inductively coupled plasma mass spectrometry with ion chromatography

Inoue, Yasuhiro; Kawabata, Katsuhiko; Takahashi, Hiromitsu; Endo, Ginji

doi:10.1016/0021-9673(94)85269-3

Cited by 58 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The operating conditions for ICP-MS were established in accordance with those reported by Inoue et al 21 The ion chromatograph was a Model IC7000 from Yokogawa Analytical Systems (Tokyo, Japan). The analytical column was a Showdex NN-614 column (150 mm Â 4.6 mm i.d.)…”

Section: Instrumentationmentioning

confidence: 99%

“…Measurements using IC-ICP-MS were performed by the method established by Inoue et al 21 with modifications. Stock standard solutions of sodium arsenite, sodium arsenate, MMA, DMA, TMAO, tetramethylarsonium iodide, and AsBe were prepared by dissolving each compound in pure water at an arsenic concentration of 100 mg l…”

Section: Ic±icp-ms Analysismentioning

confidence: 99%

“…In efforts to elucidate the biotransformation of arsenic, special attention should be paid to the analytical methods used to characterize arsenic compounds in urine. Ion chromatography with inductively coupled plasma mass spectrometry (IC-ICP-MS) 21,22 permits examination of the pattern of elimination and concentrations of several urinary arsenic metabolites simultaneously and with a high degree of sensitivity. The results obtained by IC-ICP-MS methodology eliminate analytical artifacts.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolites of arsenobetaine in rats: does decomposition of arsenobetaine occur in mammals?

Yoshida

Kuroda

Inoue

et al. 2001

Applied Organom Chemis

Self Cite

View full text Add to dashboard Cite

Biotransformation following oral administration of arsenobetaine (AsBe), the major arsenic compound in marine animals, was studied in rats. Male F344/DuCrj rats were administered a single dose of AsBe (20 mg As kg Escherichia coli, did not occur in rats. These results suggest that TMAO may be formed from AsBe, and that TMAO may subsequently be converted to the unidentified compound and demethylated arsenic compounds.

show abstract

Section: Instrumentationmentioning

confidence: 99%

Section: Ic±icp-ms Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolites of arsenobetaine in rats: does decomposition of arsenobetaine occur in mammals?

Yoshida

Kuroda

Inoue

et al. 2001

Applied Organom Chemis

Self Cite

View full text Add to dashboard Cite

show abstract

“…Arsenic speciation analysis in biological samples is generally performed using HPLC inductively coupled plasma mass spectrometry (HPLC-ICP-MS). [14][15][16] Recent publications have reported the usefulness of HPLC with tandem mass spectrometry (HPLC-MS/MS) for the identification and determination of organoarsenic compounds in biological samples. [17][18][19] These analytical apparatuses have made it possible to detect known arsenic compounds in samples without standard compounds and to estimate As concentration using appropriate standards.…”

Section: Introductionmentioning

confidence: 99%

Speciation Analysis of Arsenics in Commercial Hijiki by High Performance Liquid Chromatography-tandem-mass Spectrometry and High Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry

Shimoda

Suzuki

Endo

et al. 2010

JOURNAL OF HEALTH SCIENCE

Self Cite

View full text Add to dashboard Cite

Edible brown alga (hijiki in Japanese, Hijikia fusiforme) contains not only a high content of inorganic arsenic (iAs) but also various arsenosugars (AsSugs) which are metabolized to dimethylarsinic acid (DMA) in mammals. Since DMA is considered to be carcinogenic in rodents, it is necessary to accurately measure the contents of AsSugs as well as iAs for the risk assessment of seaweed consumption. Seven commercially available dried-hijiki products and two raw hijiki products were analyzed. Total-As was measured by inductively coupled plasma mass spectrometry (ICP-MS) with the Dynamic Reaction Cell (DRC) mode after acid-digestion. After water-extraction, AsSugs were detected by HPLC-MS/MS with multiple reaction monitoring in the positive ion mode and speciation analysis of arsenics was performed by HPLC-ICP-MS. The ranges of total-As obtained by acid digestion (A-TAs) in nine hijiki samples were 37.1-118.6 µg As/g dry weight (dw), and those of water extracted total-As (W-TAs) were 18.4-81.0 µg As/g dw. The ratios of water extracted iAs (W-iAs) to A-TAs ranged from 24.5 to 60.1%. The major compound detected was arsenate in all samples (8.9-70.5 µg As/g dw). Dimethylarsenosugar sulfate, AsSug 408, showed the highest peak among AsSugs detected. The content ratio of water extracted AsSugs (W-AsS) to A-TAs was estimated to be from 3.7 to 27.6%. The contents of A-TAs, W-iAs and W-AsS varied depending on the hijiki product. HPLC-MS/MS detected AsSugs more sensitively than HPLC-ICP-MS. Since iAs could not be detected by HPLC-MS/MS, combined analysis consisting of HPLC-MS/MS and HPLC-ICP-MS is necessary for accurate determination of arsenic species in seaweed products and also for the toxicological evaluation of AsSugs.

show abstract

“…Although the LC-ICP/MS is sensitive and precise, the LC-ICP/MS does not give any information on speciation. [4][5][6] On the other hand, the LC/MS is a good tool for qualification and quantification. We have previously reported the appropriate mobile phase and the MS conditions for the arsenic compounds using LC/MS.…”

mentioning

confidence: 99%

Liquid Chromatography-Mass Spectrometry of Arsenic Compounds Using the Electrospray Ionization with Postcolumn Addition of Methanol

et al. 1999

Self Cite

View full text Add to dashboard Cite

It has been well known that inorganic arsenic compounds can be carcinogenic to humans.1 Most mammals convert inorganic arsenic compounds to low toxicity monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA) or trimethylarsine oxide (TMAO) by methylation. 2,3 As the toxicological properties of arsenic compounds depend on their chemical forms, it has been demonstrated that the analysis of every chemical form is very important. Although the LC-ICP/MS is sensitive and precise, the LC-ICP/MS does not give any information on speciation. [4][5][6] On the other hand, the LC/MS is a good tool for qualification and quantification. We have previously reported the appropriate mobile phase and the MS conditions for the arsenic compounds using LC/MS. 7 Since it is known that the addition of an organic solvent is useful to increase the sensitivity on electrospray ionization (ESI), we examined the postcolumn addition of methanol for improvement of the linearity, detection limit and repeatability of the method. ExperimentalOrganoarsenic species MMAA, DMAA, TMAO, tetramethylarsonium (TeMA) iodide, and arsenobetaine (AB), were purchased from Tri Chemical Laboratories Inc. (Yamanashi, Japan). Other reagents (each analytical grade) were purchased from Wako Pure Chemical Industries. Deionized water was obtained from a Milli-Q system (Nihon Millipore, Tokyo, Japan). The LC/MS equipment was Model HP1100 series HPLC with MS (Hewlett-Packard, DE, USA). Electrospray ionization (ESI) mode was selected as the ionization system. A Shodex RSpak NN-614 (Showa Denko, Tokyo, Japan) packed with a hydrophilic resin-based cation exchange resin (cation exchange capacity of 0.1 meq/g) was chosen as the separation column. The separation conditions were set by referring to those of the previous paper. 7Influence of postcolumn addition of methanol on sensitivity of arsenic compounds Figure 1 shows the relationships between the flow rate of methanol added by postcolumn addition and the peak areas of arsenic compounds. The graph demonstrates that the addition of methanol to the mobile phase was effective for increasing the sensitivity, and that around 0.2 ml/min was a suitable rate for the high sensitive detection. The reason for increasing of sensitivity is that the methanol allows the surface tension of droplet from nebulizer. To decrease is consequently easier to make smaller droplets and to vaporize the liquid of droplet than without the addition of methanol. Inc., Musashino, Japan Keywords HPLC/MS, arsenic compounds, electrospray ionization Fig. 1 The relationships between the flow rates of methanol and the peak areas of arsenic compounds. Conditions: carrier, 8 mM HNO3 / 5 mM NH4NO3, 0.4 ml/min; drying gas, N2 (13 l/min, 350 ˚C); nebulizer, N2 (60 psi); accelerating voltage, 80 V; polarity, positive scan mode; sample, 10 ng/µl each, 10 µl inject; postcolumn, methanol.

show abstract

Determination of arsenic compounds using inductively coupled plasma mass spectrometry with ion chromatography

Cited by 58 publications

References 15 publications

Metabolites of arsenobetaine in rats: does decomposition of arsenobetaine occur in mammals?

Metabolites of arsenobetaine in rats: does decomposition of arsenobetaine occur in mammals?

Speciation Analysis of Arsenics in Commercial Hijiki by High Performance Liquid Chromatography-tandem-mass Spectrometry and High Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry

Liquid Chromatography-Mass Spectrometry of Arsenic Compounds Using the Electrospray Ionization with Postcolumn Addition of Methanol

Contact Info

Product

Resources

About